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METHODS article

Front. Immunol.

Sec. Inflammation

Volume 16 - 2025 | doi: 10.3389/fimmu.2025.1662986

This article is part of the Research TopicAdvances in Innate Immune Cells in chronic non-transmissible diseases: From Sensing to Effector Mechanisms in Inflammatory DiseasesView all 6 articles

Assessment of protein synthesis rate enables metabolic profiling of resident-immune cells of the islets of Langerhans

Provisionally accepted
  • 1The University of British Columbia Department of Surgery, Vancouver, Canada
  • 2BC Children's Hospital Research Institute, Vancouver, Canada
  • 3The University of British Columbia, Vancouver, Canada
  • 4University of Saskatchewan, Saskatoon, Canada

The final, formatted version of the article will be published soon.

Pancreatic islet-resident immune cells, such as lymphocytes and macrophages, support islet homeostasis, beta cell development, and tissue repair. In pathological states, including diabetes, islet immune cells can trigger inflammation, causing beta cell dysfunction and death. There has been growing interest in understanding the dynamics between beta cells and resident immune cells. Studying metabolic adaptations in beta cells and immune cells is challenging due to the mixed cell populations in islets and limited cell number, which are not suitable for conventional approaches, such as metabolomics and extracellular flux analysis. We implemented a puromycin-based flow cytometry assay for parallel analysis of the phenotype and metabolic state of islet-resident immune cells. Islets were isolated from healthy mice and exposed to a cytokine cocktail (IL-1β, TNF-α, IFN-γ) to mimic a pro-inflammatory diabetogenic microenvironment. We found that Islet-resident macrophages show higher expression of CD86 and lower expression of CD301 upon cytokine treatment, which was accompanied by reduced protein synthesis rates upon inhibition of glycolysis and mitochondrial complex V. In insulin-producing beta cells, inhibition of mitochondrial complex V (ATP synthase) by oligomycin reduces translation rates. Streptozotocin (STZ)-induced beta cell death promoted accumulation of macrophages in the islet and higher frequency of CD86+ macrophages, as was observed in vitro. Islet macrophages from STZ-treated mice showed higher basal protein synthesis rates and enhanced sensitivity to oligomycin. We validated this method in bone marrow-derived macrophages and the MIN6 beta cell line, using extracellular flux analysis as a control for the puromycin-based assay. We propose our implementation of a puromycin-based assay as a useful tool to study metabolic demands in rare islet cell populations. Applying phenotypic and protein synthesis assays coupled with specific metabolic pathway inhibitors to intact pancreatic islets can provide a better understanding of the immunometabolic cues that lead to beta cell dysfunction and failure in diabetes.

Keywords: SCENITH, single-cell energetic metabolism by profiling translation inhibition, resident macrophages, resident T cells, Pancreatic islet, Beta cell death, Immunometabolism

Received: 09 Jul 2025; Accepted: 29 Aug 2025.

Copyright: © 2025 de Brito Monteiro, Archambault, Soukhatcheva, Dai, Velghe, Chen, Verchere and Klein Geltink. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Bruce Verchere, The University of British Columbia Department of Surgery, Vancouver, Canada
Ramon I. Klein Geltink, BC Children's Hospital Research Institute, Vancouver, Canada

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