GENERAL COMMENTARY article

Front. Microbiol., 05 May 2015

Sec. Microbial Physiology and Metabolism

Volume 6 - 2015 | https://doi.org/10.3389/fmicb.2015.00362

Getting a hold on archaeal type IV pili: an expanding repertoire of cellular appendages implicates complex regulation and diverse functions

  • SC

    Scott Chimileski *

  • RT

    R. Thane Papke

  • Department of Molecular and Cell Biology, University of Connecticut Storrs, CT, USA

Type IV pili (T4P) are a group of cell surface appendages of particular interest due to broad conservation and functional versatility across the domains Bacteria and Archaea (Albers and Meyer, 2011; Giltner et al., 2012). All T4P are composed of small protein subunits known as pilins that polymerize into helical fibers through the action of assembly ATPases (Giltner et al., 2012). This core ancestral machinery has been adapted in various lineages for many cellular processes–from adhesion and biofilm formation, to motility, horizontal gene transfer (HGT) and even electricity conduction (Giltner et al., 2012; Berry and Pelicic, 2015). When T4P structures are involved in adhesion, they are known as pili, if they no longer mediate attachment, but are associated with another function, such as scavenging macromolecules (e.g., DNA uptake by Com proteins in Bacillus subtilis), or secretion of proteins through a piston-like structure (i.e., type II secretion), they are called pseudopili (Averhoff and Friedrich, 2003; Peabody et al., 2003; Chen et al., 2005). T4P appendages may also contribute to both adhesion and another function. This dual function is sometimes true for archaella: a major group of archaeal T4P appendages characterized by the ability to rotate and enable swimming motility. Archaella are functionally analogous yet structurally and genetically unrelated to bacterial flagella (Jarrell and Albers, 2012; Shahapure et al., 2014; Albers and Jarrell, 2015). T4P have been studied to a greater extent in bacteria, in part because they are often virulence factors (Giltner et al., 2012). However, recent investigations have revealed a repertoire of archaeal T4P–highlighting implications for regulatory complexity and functional diversity.

Losensky et al. (2014) demonstrated that adhesive filaments in the haloarchaeon Halobacterium salinarum R1 observed during biofilm formation (Fröls et al., 2012) are dependent on the pilus assembly ATPase gene pilB1 (Losensky et al., 2014), expanding the list of experimentally studied archaeal T4P (Table 1). Deletion of pilB1 led to a lack of pili as observed through electron microscopy and a defect in adhesion. Only 4% of a glass surface was colonized by non-piliated/non-archaellated cells (ΔflaIpilB1), relative to 36 and 44% for the parental and non-archaellated (ΔflaI) strains, respectively. The molecular composition of PilB1-dependent pili has not yet been determined, however Losensky and coauthors noted that there are over 30 candidate pilins in the Hbt. salinarum R1 genome, as indicated by the class III signal peptide prediction program FlaFind (Szabó et al., 2007b). FlaFind was used previously to show that most archaeal genomes contain many pilin/archaellin homologs (Szabó et al., 2007b; Esquivel et al., 2013). For example, Haloarcula marismortui and Haloferax volcanii have nearly 50 putative pilin/archaellin precursors (Esquivel et al., 2013).

Table 1

Structure nameFunction/associated phenotypeCharacterized in (genera)Filament diameter (nm)References
ARCHAELLA
Swimming motility and involved in adhesion in some species; functionally analogous but evolutionarily and structurally distinct from the bacterial flagellabHalobacterium10–15Alam and Oesterhelt, 1984; Gerl and Sumper, 1988; Patenge et al., 2001; Streif et al., 2008
HaloferaxTripepi et al., 2010, 2012, 2013
HaloquadratumAlam et al., 1984
SulfolobusSzabó et al., 2007a; Lassak et al., 2012b; Shahapure et al., 2014
MethanococcusBardy et al., 2002; Jarrell et al., 2011
MethanocaldococcusBellack et al., 2011
PyrococcusNather et al., 2006; Nather-Schindler et al., 2014
ADHESIVE PILI
Archaeal adhesive pilus (Aap)Surface adhesionSulfolobus11Henche et al., 2012
Type IV pilus (Epd)Surface adhesionMethanococcus8.5Vandyke et al., 2008; Wang et al., 2008; Nair et al., 2013
Type IV pilus (PilA)Surface adhesionHaloferax8–12Esquivel et al., 2013; Esquivel and Pohlschröder, 2014, 2015
PilB1-dependent adhesive pilus-likecSurface adhesionHalobacterium7–8Losensky et al., 2014
UV-inducible pilus (Ups)Autoaggregation and species specific DNA exchange following UV-irradiationSulfolobus10Fröls et al., 2008; Ajon et al., 2011
PSEUDOPILUS-LIKE
Bindosome assembly system (Bas)Sugar binding; also involved in cellular morphology and S-layer architectureSulfolobusNDZolghadr et al., 2007, 2011

Experimentally studied type IV pili in archaeal species: archaella, adhesive pili, and pseudopilia.

ND, not determined.

a

As reviewed by Pohlschröder et al. (2011), Lassak et al. (2012a), Jarrell et al. (2013) and Esquivel and Pohlschröder (2015).

b

See Jarrell and Albers (2012), Shahapure et al. (2014) and Albers and Jarrell (2015) for a review of how the archaellum came to be recognized as a unique archaeal motility structure, including a more comprehensive categorization of characterized archaella.

c

The appendages characterized by Losensky et al. (2014) are listed as pilus-like because they have yet to be purified and biochemically verified. They may in fact be composed of homologs of PilA pilin proteins in H. volcanii, which are found in many euryarchaeal species (Esquivel et al., 2013).

Some of these pilins could be associated with additional functions. Hfx. volcanii has an ability for social motility in static liquid (Chimileski et al., 2014) and T4P could be involved in this activity (Esquivel and Pohlschröder, 2015), whereby they may attach to extracellular matrix along the substratum, similar to the S-motility system that pulls Myxococcus xanthus cells forward (Hodgkin and Kaiser, 1979; Zusman et al., 2007). There could be more archaeal T4P-related surface structures that scavenge macromolecules as well, like the bindosome of Sulfolobus solfataricus (Zolghadr et al., 2007, 2011).

Investigations of archaeal T4P leave open the possibility for undiscovered mechanisms for contacting abiotic surfaces or other cells. For instance, even in the non-piliated/non-archaellated Hbt. salinarum strain, adhesion was not completely abolished (Losensky et al., 2014). Similar residual adhesion has been observed in Hfx. volcanii (Tripepi et al., 2010, 2013). In both cases, pilins that remain present in the membrane but cannot be assembled into pili without the assembly ATPase(s) likely explain low levels of adhesion (Esquivel and Pohlschröder, 2014). There are two other cell-to-cell contact phenomena in Hfx. volcanii that do not require archaella or pili: Ca2+ dependent autoaggregation (Tripepi et al., 2010), and an HGT mechanism known as mating (Rosenshine et al., 1989; Tripepi et al., 2010; Naor et al., 2012). Additional types of extracellular polymers or fibers found in bacterial species could be present in archaea, such as amyloid protein (Chimileski et al., 2014). Unusual, genetically ambiguous non-T4P structures have already been observed in other archaeal species, including the hamus of the SM1 euryarchaeon (Moissl et al., 2005; Perras et al., 2014) and the cannulae of Pyrodictium cells (Nickell et al., 2003).

A plausible explanation for having a wide array of appendages is a capacity for differential regulation (Jarrell, 2012; Lassak et al., 2012a; Jarrell et al., 2013). Indeed, a number of studies point to dynamic regulatory systems controlling archaeal T4P. In Hbt. salinarum, pilB1 expression was upregulated relative to flaI in adherent cells (Losensky et al., 2014), suggesting archaella and pili have antagonistically regulated functions in motility (when a planktonic state is favorable) and for adhesion (during biofilm formation), as in Hfx. volcanii (Tripepi et al., 2010; Esquivel and Pohlschröder, 2014, 2015). Haloarcula marismortui has two archaellins that are expressed under different temperatures and salinities (Syutkin et al., 2014), termed ecoparalogs. Likewise, the six Hfx. volcanii pilA paralogs, any one of which can restore adhesion when expressed in a null mutant [ΔpilA(1–6)] (Esquivel et al., 2013), may be ecoparalogs as well. Intriguingly, deleting flgA2, one of two archaellin genes in Hfx. volcanii, produced a hypermotile phenotype, rather than a motility defect (Tripepi et al., 2013). Archaellins are also regulated through N-glycosylation (Guan et al., 2012; Tripepi et al., 2012) and regulatory proteins controlling adhesive pili and archaella have been identified in Sulfolobus acidocaldarius (Reimann et al., 2012; Orell et al., 2013; Vassart et al., 2013).

As more T4P are described in archaeal groups, a common theme is appearing. A multitude of individual pilins/archaellins from one or more loci may appear to be redundant–contributing to appendages that are difficult to differentiate through electron microscopy and often depend on the same assembly ATPase. However, to the contrary, the maintenance of more than one pilus and archaellum subunit gene is likely due to a complex regulatory network and the corresponding advantages of functional versatility. Subsets of pilins may be expressed in different combinations as a response to a variety of specific environmental conditions and/or cellular functions.

Statements

Acknowledgments

RTP was supported by the National Science Foundation (award numbers DEB-0910290 and DEB-0830024) and the NASA Astrobiology: Exobiology and Evolutionary Biology Program (grant number NNX12AD70G).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Summary

Keywords

haloarchaea, type IV pili, archaellum, surface adhesion, archaeal biofilm formation, microbial cell-to-cell interactions

Citation

Chimileski S and Papke RT (2015) Getting a hold on archaeal type IV pili: an expanding repertoire of cellular appendages implicates complex regulation and diverse functions. Front. Microbiol. 6:362. doi: 10.3389/fmicb.2015.00362

Received

08 January 2015

Accepted

10 April 2015

Published

05 May 2015

Volume

6 - 2015

Edited by

Biswarup Mukhopadhyay, Virginia Tech, USA

Reviewed by

Sonja-Verena Albers, University of Freiburg, Germany; Jerry Eichler, Ben-Gurion University of the Negev, Israel; Mechthild Pohlschröder, University of Pennsylvania, USA

Copyright

*Correspondence: Scott Chimileski,

This article was submitted to Microbial Physiology and Metabolism, a section of the journal Frontiers in Microbiology

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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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