Skip to main content


Front. Microbiol., 04 August 2017
Sec. Plant Pathogen Interactions
This article is part of the Research Topic Rhizosphere Bacterial Community and Bacteria-Plant Interactions View all 14 articles

Comparative Transcriptomics of Bacillus mycoides Strains in Response to Potato-Root Exudates Reveals Different Genetic Adaptation of Endophytic and Soil Isolates

  • Molecular Genetics Department, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands

Plant root secreted compounds alter the gene expression of associated microorganisms by acting as signal molecules that either stimulate or repel the interaction with beneficial or harmful species, respectively. However, it is still unclear whether two distinct groups of beneficial bacteria, non-plant-associated (soil) strains and plant-associated (endophytic) strains, respond uniformly or variably to the exposure with root exudates. Therefore, Bacillus mycoides, a potential biocontrol agent and plant growth-promoting bacterium, was isolated from the endosphere of potatoes and from soil of the same geographical region. Confocal fluorescence microscopy of plants inoculated with GFP-tagged B. mycoides strains showed that the endosphere isolate EC18 had a stronger plant colonization ability and competed more successfully for the colonization sites than the soil isolate SB8. To dissect these phenotypic differences, the genomes of the two strains were sequenced and the transcriptome response to potato root exudates was compared. The global transcriptome profiles evidenced that the endophytic isolate responded more pronounced than the soil-derived isolate and a higher number of significant differentially expressed genes were detected. Both isolates responded with the alteration of expression of an overlapping set of genes, which had previously been reported to be involved in plant–microbe interactions; including organic substance metabolism, oxidative reduction, and transmembrane transport. Notably, several genes were specifically upregulated in the endosphere isolate EC18, while being oppositely downregulated in the soil isolate SB8. These genes mainly encoded membrane proteins, transcriptional regulators or were involved in amino acid metabolism and biosynthesis. By contrast, several genes upregulated in the soil isolate SB8 and downregulated in the endosphere isolate EC18 were related to sugar transport, which might coincide with the different nutrient availability in the two environments. Altogether, the presented transcriptome profiles provide highly improved insights into the life strategies of plant-associated endophytes and soil isolates of B. mycoides.


The rhizosphere harbors an enormous pool of soil microorganisms and is considered as the ‘hot spot’ for microbial colonization and activity (Prashar et al., 2013). Some of the rhizobacteria even have the capacity of multiplying inside roots and begin an endophytic lifestyle (Hardoim et al., 2008). Rhizobacteria and plants naturally interact in various ways. Beneficial plant–microbial interactions in the rhizosphere can result in the promotion of plant health and development (Mark et al., 2005). A number of plant growth-promoting rhizobacteria (PGPR) have been commercially used as adjuncts to agricultural practice and show great promise (Glick, 2012). The mechanisms of PGPR and plant root interaction have drawn considerable attention (Kuiper et al., 2004; Santos et al., 2014; Pangesti et al., 2015). However, only a few studies on plants and their interaction with endophytic bacteria have been reported so far.

Endophytic bacteria are defined as bacteria capable of colonizing the internal tissue of the plant without harming the host or causing any disease symptoms (Mónica and Esperanza, 2006). It has been shown that many endophytic bacteria can promote plant growth with a similar mechanism as rhizobacteria, e.g., phosphate solubilisation (Wakelin et al., 2004), indole acetic acid production (Lee et al., 2004; Jasim et al., 2014), and siderophore production (Rosconi et al., 2013). A number of endophytic bacteria are able to prevent the deleterious effects of certain pathogens by producing antimicrobial compounds or inducing systematic resistance in the host plant (Gómez-Lama Cabanás et al., 2014; Kandel et al., 2017). The beneficial effects of endophytes are often more pronounced than those of many rhizosphere-colonizing bacteria (Hardoim et al., 2008). A large diversity of bacterial species have the ability to adopt an endophytic lifestyle. These comprise more than 200 bacterial genera from 16 phyla, with Actinobacteria, Proteobacteria, and Firmicutes being the most predominant genera (Malfanova et al., 2013; Jin et al., 2014). The rhizosphere-competent bacteria, including Azospirillum, Bacillus, and Pseudomonas genera, are most frequently reported as plant internal tissue residents. In fact, the endophytic bacterial diversity can be considered as a subset of the rhizosphere and/or the root-associated bacterial population (Santoyo et al., 2016). This implies that only a limited number of members of the rhizosphere-competent bacteria can succeed in becoming endophytic. This is due to several factors: (1) plants can selectively recruit certain beneficial rhizobacteria (Lakshmanan et al., 2013); (2) only those rhizobacteria that are well-adapted for living inside plants and simultaneously show an aggressive colonization ability can become endophytes. It has been reported that several Rhizobium etli strains were preferentially encountered as maize endophytes and the highly competitive strain Ch24-10 was the most tolerant to 6-methoxy-2-benzoxazolinone, a maize antimicrobial compound that is inhibitory to some bacteria and fungi (Rosenblueth and Martínez-Romero, 2004). Pseudomonas strains that were isolated from the endosphere are phenotypically distinct from Pseudomonas isolates obtained from the root surface as identified by their lipopolysaccharide pattern, cell envelope protein pattern, and other biochemical characteristics (van Peer et al., 1990). A comparative genomics study by Timm et al. (2015) showed that endosphere isolates of P. fluorescens have significantly more metabolic pathways for utilization of plant signaling compounds than rhizosphere isolates.

During initiation of root surface colonization by rhizosphere microorganisms, the first event in the interaction with plants is the encountering of root exudates (Shidore et al., 2012). Plant roots can exude up to 20% of fixed carbon into the soil. Amino acids, organic acids, sugars, aromatics, and various other secondary metabolites comprise the majority of the low-molecular-weight root exudates, whereas high-molecular-weight exudates primarily include polysaccharides and proteins (Brencic and Winans, 2005). Microbes can use these compounds as carbon- and energy sources for growth and development. Apart from being an exogenous nutrition, some molecules from exudates may act as signals to alter specific gene expression patterns in the microbe, which might influence its interaction with the host (Mark et al., 2005). For example, the plant-released molecules, including flavonoids, stachydrine, and trigonelline are recognized as signals for induction of nodulation genes in various legume-associated bacteria (Phillips et al., 1992; Janczarek et al., 2015). The citric acid in cucumber exudates was shown to recruit B. amyloliquefaciens SQR9 and to induce its biofilm formation (Zhang et al., 2014). Root colonization by endophytic bacteria is hypothesized to follow a similar process as described for rhizosphere bacteria. It has been shown that plant compounds from leaf macerates can activate a LuxR family transcription factor and its regulated genes of the cottonwood tree endophyte Pseudomonas sp. GM79 (Schaefer et al., 2016). Thus, molecules released from plants might play an important role in the plant–endophyte interaction.

Bacillus mycoides is a spore-forming, gram-positive bacterium belonging to the B. cereus sensu lato group. Among this group, B. mycoides is probably the least recognized and unresearched species, since it is in contrast to B. cereus and B. anthracis not pathogenic and does not have insecticidal activity as for instance B. thuringiensis. On agar plates, B. mycoides forms unique rhizoid colonies with chains of cells forming filaments projecting radially and curving to the left or the right (Di Franco et al., 2002). B. mycoides is ubiquitous in the soil and the rhizosphere. Some isolates have beneficial plant growth promoting (PGP) and biocontrol activity in various plants, including sunflower (Ambrosini et al., 2016), cucumber (Neher et al., 2009), and sugar beet (Bargabus et al., 2002). Our research showed that certain B. mycoides strains were able to endophytically colonize potato roots with potential PGP activity (unpublished data). However, the mechanisms of the beneficial interaction between the host and this endophyte are not known. Transcriptomics is a powerful approach to investigate plant–microbe interactions, but in situ transcriptomics of plant–microbe interaction models has limitations due to the imbalances of biomass ratios. As an alternative, root exudates or similar simplified systems are therefore used to study the bacterial response in the symbiotic interaction models. Root exudates were shown to have profound effects on gene expression patterns of rhizosphere bacilli including B. amyloliquefaciens FZB42 (Fan et al., 2012) and B. amyloliquefaciens SQR9 (Zhang et al., 2015). A transcriptome analysis conducted with the endophytic bacterium Azoarcus sp. strain BH72 and Oryza sativa root exudates, identified several genes required for rhizosphere competence (Shidore et al., 2012). Taghavi et al. (2015) used sucrose as sole carbon source to mimic the plant-associated lifestyle of Enterobacter sp. 638. Their transcriptome study revealed that the small RNA csrABCD regulon plays a role in the physiological adaptation and possibly mediates the shift between the free-living and a plant-associated endophytic lifestyle.

In this study, we compared the transcriptome profiles of an endophytic strain and a soil strain of B. mycoides in response to potato root exudates. The aim was to investigate differences in the genetic responses of the endophytic and the soil isolate and to decipher the endophytic determinants of B. mycoides. The colonization ability of the endophyte strain EC18 and the soil strain SB8 was tested by plant inoculation assays and in situ fluorescence microscopy monitoring. The whole genome of the two strains was sequenced and used as a reference for the transcriptome analysis. B. mycoides strain EC18 and SB8 were grown in the presence or absence of root exudates of potato plants, and differences in their gene expression profiles were analyzed by RNA-seq. Some genes with specific functionality showed a distinct trend between endophyte and soil strain and may be considered as key factors determining the endophytic lifestyle of B. mycoides.

Materials and Methods

Strains and Culture Condition

Bacillus mycoides EC18 was isolated from internal root tissue of the potato cultivar Seresta during the early flowering stage, grown on a practice field in a sandy soil at Wijster, the Netherlands. The soil strain SB8 was isolated from bulk soil of the same area. For isolation, samples were spread on Nutrient Broth agar plates (NB, 0.5% peptone, 0.3% yeast extract, 0.5% NaCl, 1.5% agar) supplemented with 0.1 mg/ml cycloheximide to inhibit fungal growth and incubated at 28°C for 24 h. The strains were identified by particular rhizoid colony morphology and 16S rRNA gene sequence comparison. After isolation, B. mycoides strains were grown in Luria-Bertani (LB, 1% tryptone, 0.5% yeast extract, 0.5% NaCl) or Brain Heart Infusion (BHI, BactoTM, BD Biosciences, France) medium at 30°C with 200 rpm agitation. E. coli strain MC1061 was used for cloning and was grown in LB at 37°C with 220 rpm agitation. When needed, 50 μg/ml of kanamycin or 100 μg/ml of ampicillin (E. coli) or 4 μg/ml of chloramphenicol (B. mycoides) were added to the growth media.

Construction of GFP- and RFP-tagged B. mycoides

The vector PYB was constructed by replacing the pAMß1 origin of replication (ori) of plasmid PATΔS28 (Namy et al., 1999) with the gram-positive temperature sensitive ori PWV01 from PAW068 plasmid (Wilson et al., 2007). Thus, the new vector PYB contains a PUC ori, a spectinomycin resistance gene, and a gram-positive temperature sensitive ori. A 1 kb fragment of the α-amylase gene was amplified from the genome of B. mycoides and digested with the restriction enzymes SacI and KpnI and ligated into the corresponding site of PYB resulting in the plasmid PYB_amy. The green fluorescent protein (GFP) and red fluorescent protein (RFP) genes were cloned into PYB_amy to obtain the vectors PYB_amyGFP and PYB_amyRFP. The plasmid PYB_amyGFP was transformed into B. mycoides EC18 and PYB_amyRFP was transformed into strain SB8 by electroporation as described previously (Yi and Kuipers, 2017) and colonies were selected on 100 μg/ml spectinomycin LB plates at 30°C. Transformants expressing GFP or RFP were selected by an Olympus MVX10 MacroView fluorescence microscope. Several bright colonies were inoculated in BHI liquid medium and grown at 37°C to achieve a single crossover recombination. The cultures were diluted and plated on a BHI agar plate with 100 μg/ml spectinomycin and incubated at 37°C. Successful GFP and RFP integration was verified by colony PCR. The expression of the fluorescent protein was analyzed by fluorescence microscopy (Nikon Eclipse Ti, Japan) equipped with a CoolsnapHQ2 CCD camera. Images with GFP- or RFP- fluorescent cells were taken using 450–490 or 560–600 nm excitation for fluorescence channels and an Intensilight light as phase contrast channel. Final pictures were generated by ImageJ software1 by merging the two channels.

Root Colonization of the Endophyte and Soil Strains Observed by Confocal Laser Scanning Microscopy (CLSM)

The overnight culture of GFP-tagged EC18 and RFP-tagged SB8 were diluted 50 times and the strains were grown in BHI liquid medium at 37°C to the exponential growth phase. Cells were collected by centrifuging at 3000 × g and cell pellets were washed by 10 mM MgSO4 twice and resuspended in 10 mM MgSO4 to an OD600 of 0.65. The viable cells were counted by counting colonies on LB agar plate containing 100 μg/ml spectinomycin. The densities of EC18 and SB8 suspension were 1.2 × 108 to 1.4 × 108 cfu ml-1. The plant root inoculation protocol was modified from Prieto et al. (2011). Briefly, Chinese cabbage seeds were surface sterilized by soaking in 2–3% sodium hypochlorite and 70% ethanol for 2 min, respectively. Seeds were washed with sterilized water three times to remove the remaining disinfectant and germinated on 25% Hoagland medium solidified with 0.6% agar. After 7 days growing in a climate chamber at 25°C and 16/8 h light/dark photo-period, plant root was dipped for 45 min in bacterial suspensions of GFP-tagged B. mycoides EC18, RFP-tagged B. mycoides SB8, and a 1:1 mixture of the two strains. Plants were then transferred to solid Hoagland medium plates and incubated in a climate chamber at 25°C and 16/8 h light/dark.

For the visualization of the plant colonization by B. mycoides, fresh roots of cabbage plants were collected at 2 and 3 days after bacterial inoculation. The whole root was mounted on a glass slide and imaged using a confocal laser scanning microscope (Zeiss LSM 800, Carl Zeiss, Germany). Lasers at the wavelength of 488 and 561 nm were used to excite GFP and RFP, respectively. In order to visualize the endophytic colonization, a three-dimensional model that was rendered from about 60 optical sections at 1 μm intervals, was obtained with the Zen lite software (Carl Zeiss, Germany).

Genomic DNA Extraction, Sequencing, and Annotation

Bacillus mycoides strains were grown overnight in BHI broth at 30°C, 200 rpm. One ml of overnight culture was centrifuged and the pellet was resuspended in 250 μl SET buffer (75 mM NaCl, 25 mM EDTA, and 20 mM Tris-HCl at pH 7.5). The cells were lysed with lysozyme at 37°C for 30 min. Then 10 μl of 10 mg/mL RNase was added and incubated 10 min at room temperature to remove RNA. After that, 25 μl SDS (10%) and 10 μl proteinase K (20 mg/ml) were added and the cells were incubated at 55°C for 30 min. The lysate was treated with phenol–chloroform and the water phase was used to recover DNA by isopropanol precipitation. Precipitated DNA was washed two times with cold 70% ethanol and finally dissolved in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5). The quality and quantity of the genomic DNA was checked with a Nanodrop (ND-1000, Thermo Scientific, United States) and a Promega fluorometer (Quantus, Promega, United States).

Purified genomic DNA was sequenced using the MiSeq sequencing system of Illumina (Zerbino and Birney, 2008), yielding 250 bp paired-end reads with a mean library size of 400 bp. De novo assembly was performed using Velvet (Zerbino and Birney, 2008). Prediction of protein-encoding regions and automatic functional annotation was performed using the Rapid Annotations using Subsystem Technology (RAST) server (Aziz et al., 2008). Genes for tRNAs and rRNAs were predicted with tRNAscan-SE (Lowe and Eddy, 1997) and RNAmmer server (Lagesen et al., 2007) respectively, with the prokaryotic default setting. Insertion sequences (ISs) were identified by BLASTN searches against the ISfinder database (Siguier et al., 2006). Prophage regions were identified using the PHAST web server (Zhou et al., 2011). Orthologous protein coding sequences in B. mycoides ATCC 6462, B. mycoides EC18, and B. mycoides SB8 were analyzed by the OMA (Orthologous Matrix) orthology database (Altenhoff et al., 2014). In addition, the functionality analysis of all the genes was performed by searching against protein domain databases, including GO (Harris et al., 2004) and COG (clusters of orthologous groups) (Tatusov et al., 2003).

Root Exudate Preparation and Bacterial Treatment

For the collection of potato root exudates, tubers were rinsed with water to remove surface particles. Clean tubers were bathed in 2–3% sodium hypochlorite and 70% ethanol for 2–3 min, followed by five washing steps with sterile distilled water. The tubers were then sowed in autoclaved pots containing wet vermiculite and kept in a climate chamber at 26°C, with a photoperiod of 16 h of light and 8 h of dark for 2 weeks. When the shoots sprouted, the seedlings were transferred into beakers filled with 20 ml of autoclaved water. The potato tubers were fitted in small plastic baskets, which support the tubers being just above the water surface and the roots hanging in the water. The water containing the exudates was collected and the beakers were refilled with sterile water. Sample collection was performed for 7 days after transferring the seedlings. A 100 μl aliquot of each collection was taken and spread on an LB plate to check for contamination and the contaminated samples were discarded. The other samples were stored at -20°C until use. An overnight culture of B. mycoides was diluted to an initial OD600 of 0.05 in 90 ml LB medium supplemented with 10 ml of root exudates collected from potato plants. The same amount of Mili-Q water served as control. Ten milliliter of root exudates were selected, since no discernible effect on bacterial growth was observed at this level. The cultures were grown at 30°C, 200 rpm for 1 h and cells were harvested for RNA isolation.

RNA Extraction

The total RNA was isolated with the Roche RNA isolation kit with some modifications. The cell pellets were flash-frozen in liquid nitrogen and resuspend in 400 μl TE buffer in 2 ml screw-cap tube. Then 50 μl of 10% SDS, 250 μl chloroform, 250 μl phenol and 0.5 g glass beads (0.5 μm) were added. The samples were homogenized using a Mini-BeadBeater (607, BioSpec, United States) for three 45 s cycles and were chilled on ice between cycles. Following the disruption, samples were centrifuged at 4°C and the supernatant was mixed with chloroform. After centrifugation at 4°C for 5 min, 500 μl of the upper phase were transferred to a fresh tube and 1 ml of lysis/binding buffer from the Roche RNA isolation kit were added. The mixture was transferred to filter and collection tubes and centrifuged 15 s at 8600 rpm. The filter tube was dried by an additional centrifugation step for 15 s at 8600 rpm. DNaseI solution was added and DNA was removed by incubation at 15–25°C for 20–30 min. After two washing steps with the wash buffer (Roche RNA isolation kit), the RNA was eluted with 50 μl elution buffer (Roche RNA isolation kit) and stored at -80°C before sequencing. The RNA quality was analyzed with the Bioanalyzer (Agilent Technologies, Wilmington, DE, United States).

RNA Sequencing and Data Analyses

RNA sequencing was conducted at PrimBio Research Institute (Exton, PA, United States). The raw RNA-Seq reads were trimmed from the adapter sequences and mapped against the reference genome sequence of each strain. The RPKM (Reads Per Kilobase Million) table of the core genome of the three strains (4743 genes) was generated. Differential gene expression analyses were carried out with the web server pipeline T-REx (de Jong et al., 2015), in which the RPKM values of the B. mycoides strain grown in the presence of root exudates was compare with that grown in the absence of root exudates. The significance threshold was p-value < 0.05 and fold-change > 2 (“TopHits” in T-Rex). Functional categorization including GO, InterPro, KEGG and PFAM domains, was performed on the Genome2D web server2. The RNA-seq data from this study have been submitted to the NCBI Gene Expression Omnibus (GEO3) under the accession number GSE98148.

Results and Discussion

Root Colonization of Endophytic and Soil-Associated B. mycoides Strains

In order to generate traceable B. mycoides strains for plant-interaction imaging experiments, the plasmid PYB_amyGFP was transformed into the endophytic B. mycoides strain EC18 by electroporation. A GFP gene controlled by a constitutive promoter was integrated in the α-amylase locus by curing the thermo-sensitive plasmid backbone at 37°C. A RFP gene was introduced into the strain SB8 with the plasmid PYB_amyRFP by appplying the same procedure. In order to compare the rhizosphere competence of the B. mycoides EC18 cells expressing GFP (Figure 1A) and of the SB8 cells expressing RFP (Figure 1B), a 1:1 mixture of the strains was inoculated into the hydroponic plant interaction model, and strain competition experiments were performed (Figure 1C). Inoculated cabbage plants were grown in a climate chamber and root samples were taken 1 and 3 days after inoculation (DAI). Subsequently, samples were examined for the presence of bacterial cells using confocal laser scanning microscopy (CLSM). When the strains were inoculated separately, both the endophytic strain B. mycoides EC18 (Figure 1D) and the soil strain B. mycoides SB8 (Figure 1E) were detected in the rhizosphere 1 DAI. Remarkably, when a 1:1 mixture of the EC18 and SB8 cells was inoculated, the roots were predominantly colonized by EC18 (Figure 1F). After 3 days of inoculation, colonization of the roots was progressing and the attachment of cells was significantly increased in the EC18 single inoculation group (Figure 1G). A similar trend was also observed for the SB8 population, albeit less significantly (Figure 1H). In the mixed inoculation group, the two strains shared a few colonization sites, however, the EC18 micro-populations predominated over the SB8 micro-populations (Figure 1I). A three-dimensional model showed that B. mycoides EC18 was able to colonize cortical tissues and xylem vessels 3 DAI (Figure 1J). In the mixture inoculation group, both strains were detected on the root surface, but only EC18 was detected inside the root tissues (Figure 1L). In the B. mycoides SB8 single inoculation experiment, endophytic colonization was not detected (Figure 1K).


FIGURE 1. Root colonized by Bacillus mycoides observed by CLSM. GFP-tagged B. mycoides EC18 (A), RFP-tagged B. mycoides SB8 (B), and a 1:1 mixture of the two strains (C) were inoculated to the seedlings. Root samples were inspected 1 and 3 days after inoculation (DAI). (D,G) Root inoculated with B. mycoides EC18 at 1 and 3 DAI. (E,H) Root inoculated with B. mycoides SB8 at 1 and 3 DAI. (F,I) Root inoculated with a 1:1 mixture of B. mycoides EC18 and B. mycoides SB8 at 1 and 3 DAI. (JL) Three dimensional model of roots inoculated with EC18, SB8, and a 1:1 mixture of the two strains 3 DAI.

As soil-borne microorganisms, several rhizosphere Bacillus species can enter plants and colonize internal tissues and many of them have shown PGP effects (Ji et al., 2008; Yi et al., 2013; Harun-Or-Rashid et al., 2017). The ability to colonize the rhizoplane is required for a successful endophytic colonization. As shown in Figures 1D,E,G,H, although both the EC18 and the SB8 isolates can establish a rhizoplane colonization, EC18 showed a more successful, faster and aggressive attachment. Following root attachment, the endophytic B. mycoides strain used a penetration process to enter the root interior and initiate the endophytic cell development. It has been discussed that some rhizosphere bacteria can occasionally and temporarily become endophytic, since passive penetration can take place at areas with damaged tissue, such as those occurring at root emergence sites or root tips (Compant et al., 2010). However, our data indicate that specific cell adaptations might be involved in active penetration of the root system by B. mycoides, because EC18 and SB8 colonized the same sites of the root, but only EC18 was able to enter the root tissue (Figure 1L).

Genomic Analyses of B. mycoides Stains

The Whole Genome Shotgun sequence of strains EC18 and SB8 has been deposited at GenBank under the accession MRWW00000000 and MRWS00000000, respectively. The general features of the genomes of EC18, SB8 and the reference strain ATCC 6462 are listed in Table 1. EC18 has a genome size of 5.75 Mb (Mega Base pairs) and SB8 has a genome size of 5.98 Mb, both of which are bigger than the type strain ATCC 6462 (5.64 Mb). The GC content of all three strains is around 35%. EC18 encodes 6,014 proteins, 77 RNA genes (7 rRNA and 70 tRNA genes) and 71 prophage-associated genes, while the SB8 genome comprises 6,250 protein-coding genes, 47 RNA genes (3 rRNA and 44 tRNA genes), and 177 prophage-associated genes. As shown in Figure 2, the core genome of the three strains consists of 4,743 orthologous groups and a pan-genome size of 7,234 genes, among which 357 genes are unique to EC18 and 646 genes are unique to SB8. 810 orthologous genes are shared by EC18 and SB8, 104 genes are shared between EC18 and ATCC 6462, while only 51 genes are shared between SB8 and ATCC 6462. The fact that SB8 and EC18 share 810 orthologous genes distinct from ATCC 6462, suggests that the latter strains are more closely related to each other than to the type strain isolate.


TABLE 1. A comparison of the genomic features of Bacillus mycoides strains ATCC 6462, EC18, and SB8.


FIGURE 2. Venn diagram showing the core genome and the genes specific of the B. mycoides strain ATCC 6462, EC18, and SB8.

Global Transcriptional Profiles in Response to Root Exudates

About 10 million reads were generated by RNA-Seq for each sample, of which 60–70% passed the quality filtering (Phred quality scores of > 20) using the FASTX-Toolkit version Subsequently, the filtered reads were mapped on the genome of type strain B. mycoides ATCC 6462 to deduce the RPKM value of each gene or RNA. The RPKM values of the core genes (4,743 genes) were used to compare the transcriptomes of the strains under study using the T-REx pipeline (de Jong et al., 2015). Significant differentially expressed genes (DEGs) were shown in the TopHits genes close to cutoff values (p-value 0.05 and fold-change of 2) (Figures 3A,B).


FIGURE 3. Analyses of differentially expressed genes (DEGs) of B. mycoides in response to potato root exudates. (A,B) Volcano diagrams of DEGs of EC18 and SB8. Spots in the right and left quadrant indicate significantly up and down regulated genes, respectively. Spots in the gray area were neither over- or under-expressed genes. (C,D) The number of up and downregulated DEGs of B. mycoides EC18 and SB8. (E) Venn diagram showing the numbers of overlapped and non-overlapped DEGs.

In the endophytic strain EC18, 715 genes were significantly changed by the exposure to root exudates. Of those, 408 genes were upregulated and 307 genes were downregulated (Figure 3C). 193 out of the 715 altered genes were represented in the COG database and hence used to perform ortologanalyses. As shown in Figure 4, a large part of the genes are poorly characterized and belong to the group [R] (General function prediction only) or [S] (Function unknown). Apart from this, the upregulated genes were mainly implicated in amino acid metabolism and signal transduction, while many of the significantly downregulated genes were involved in cell cycle control, amino acid transport and amino acid metabolism, cell wall and membrane biogenesis, transcription, and carbohydrate transport and metabolism. The soil strain SB8 responded to the root exudates with an upregulated of 261 genes,whereas 219 genes were significantly downregulated. 109 of the in total 480 altered genes were found in the COG database. The largest group of altered genes were again clustered in [R] and [S], thereby leaving their function unknown or only providing an insight into a generally assumed function. The genes represented by COG classes with known function were mostly categorized in amino acid transport and metabolism, cell cycle control, and energy production and conversion. Taken together, the results showed clear differences in gene expression changes between EC18 and SB8 in response to potato root exudates.


FIGURE 4. Functional categories of genes regulated of strain EC18 and SB8 according to COGs (Clusters of Orthologous Groups). Categorization of all altered genes was done on the Genome2D server (

DEGs Showed Overlaps between Endophyte and Soil Isolate

We next analyzed the DEGs that showed an overlap and similar changes in expression level modifications between the EC18 and SB8 isolates (Figure 3). In total, 115 genes were modulated in a similar pattern: 61 genes were upregulated and 54 genes were downregulated on both strains. While the expression of two membrane proteins was upregulated, the transcript levels were significantly lowered for a third membrane protein. Genes related to sugar transport (two genes), transcription regulators (three genes) and central intermediary metabolism (three methyltransferase genes) were also altered in the same pattern (Figure 5). One gene encoding a multidrug efflux protein (BG05_RS09165) was upregulated in both strains. It has been reported that multidrug efflux pumps are important for processes of detoxification of intracellular metabolites in plant host (Martinez et al., 2009). Similar studies with other rhizosphere-associated Bacillus species have shown that the sporulation and germination process can be affected by root exudates (Fan et al., 2012; Zhang et al., 2015). In line with these findings, nine sporulation and two germination genes were altered in the two strains in this study. This indicates that modulation of sporulation and germination pathways in response to root exudates is a response of Bacillus species independent from their ecological niche as a rhizosphere, endosphere, or soil strain. Furthermore, five genes with oxidoreductase activity were both downregulated by root exudates in the two strains.


FIGURE 5. Overlapping DEGs of B. mycoides strain EC18 and SB8 in response to root exudates. Red represents significant upregulation and blue indicates significant downregulation of gene expression.

DEGs Showed Opposite Trends between Endophyte and Soil Isolate

A total of 105 DEGs showed a different pattern between EC18 and SB8. 53 genes were upregulated in EC18 but downregulated in SB8. On the contrary, 52 genes were downregulated in EC18 but upregulated in SB8 (??). The different expression profiles suggest that endophytic and soil-associated B. mycoides apply different genetic adaptation strategies to recognize or respond to plant-released signals. Presumably, The endophytic strain is able to change its gene expression pattern and thus adapt the metabolism toward a physiological state that enables an optimal nutrient acquisition, competition with species from the same niche and colonization of the plant.

As shown in Figure 6, the genes being upregulated in EC18 and downregulated in SB8 included transcriptional regulators (five genes) among which IclR is related to multidrug resistance and degradation of aromatic compounds in soil bacteria (Song et al., 2004; Molina-Henares et al., 2006) and the sigma-28 factor is reported to transcribe the flagellin gene and control the transcription of a regulon specifying flagellar, chemotaxis, and motility functions in B. subtilis (Helmann and Chamberlin, 1987; Mirel and Chamberlin, 1989). Additionally, genes involved in the metabolism of amino acids showed an oppositely regulated expression pattern between the two strains. These included two genes involved in tryptophan metabolism (BG05_24905 and BG05_24920), three genes involved in branched-chain amino acid metabolism, e.g., BG05_22255 (ilvB), BG05_22250 (ilvC), and BG05_22265 (ilvE), and two genes involved in cysteine/methionine metabolism (BG05_11740 and BG05_23955). Xie et al. (2015) reported that several genes related to amino acids metabolism were stimulated when B. subtilis OKB105 was incubated with rice seedlings for 2 h. This finding is perhaps not surprising for rhizobacteria and endophytic bacteria, since amino acids are one of the major constituents of plant root exudates (Lugtenberg et al., 2001; Mark et al., 2005). However, it is interesting to see that those genes are downregulated in the soil strain when it encounters the root exudates.


FIGURE 6. Differentially expressed genes show opposite trends in B. mycoides strain EC18 and SB8 in response to root exudates. Red represents significant upregulation and blue indicates significant downregulation of gene expression.

Another group of genes was upregulated in the soil-derived strain SB8 and downregulated in endophytic strain EC18. These included two transporters involved in multidrug resistance proteins (BG05_26905 and BG05_26390) and several transporters including an major facilitator superfamily (MFS) transporter (BG05_17370), a citrate transporter (BG05_28360), a cellobiose PTS transporter (BG05_24345), and a putrescine importer (BG05_24345). Notably, a previous study has shown that the increased rate of putrescine uptake can decrease the competitive root colonization ability of Pseudomonas fluorescens WCS365 (Kuiper et al., 2001). The upregulation of this gene might thus decrease the rhizosphere colonization competence of SB8.

DEGs Exclusively Changed in the Endophyte or the Soil Isolate

In addition to the overlapping transcriptional responses of the two strains, there is a large number of genes that were specifically modulated in one of the two strains studied. As shown in Figure 4, 495 DEGs were identified as endophytic strain-specific responses (294 up- and 201 downregulated) and 260 DEGs were identified as soil strain-specific responses (148 up- and 112 downregulated). Some of these genes have unknown functions and are currently annotated as hypothetical proteins (141 for EC18 and 91 for SB8). Altogether, this large number of specialized transcriptional changes underpins the different and specialized responses of endophytic and soil B. mycoides strain to the plant signals. We selectively discuss some genes that may participate in B. mycoides–plant interactions. In general, the endophytic strain showed a more complex response than the soil isolate. The DEGs are categorized based on GO, COG, and Pfam databases.

The endophytic strain responded to the root exudate with a significant upregulation of genes related to amino acid transmembrane transport, while genes involved in sugar transport including BG05_RS27120 (cellobiose PTS transporter IIA) and BG05_RS26300 (arabinose ABC transporter permease) were downregulated (Figure 7). However, as shown in Figure 8, several genes related to the PTS (phosphohistone-sugar phosphotransferase system) were significantly upregulated in the soil isolate SB8, i.e., those genes including sugar ABC transporter (BG05_RS11820), PTS lactose transporter subunit (BG05_RS06025), PTS cellobiose transporter subunit (BG05_RS27110 and BG05_RS27115), and PTS sugar transporter (BG05_RS27275). It was reported that some rhizosphere bacteria selectively and preferentially consume amino acids instead of sugars. The catabolite repression of glucose assimilation in the presence of amino acids was observed among the rhizosphere genera Pseudomonas and Micromonospora (Hoskisson et al., 2003; Moreno et al., 2009). Moreover, it has been shown that the enhanced ability to catabolize amino acids is responsible for the fitness gain during nutrient-limiting conditions (Zinser and Kolter, 1999). This suggests that bacteria, which are capable of modulating the amino acid uptake and preferring their utilization may have an selective advantage under such conditions. In this study, we observed that even within the same species, an endophytic strain and a soil strain show different metabolic preferences for amino acids or sugars due to the adaptation of the endosphere or soil niche.


FIGURE 7. Differentially expressed genes specifically present in endophytic strain B. mycoides EC18 in response to root exudates. Red represents significant upregulation and blue indicates significant downregulation of gene expression.


FIGURE 8. Differentially expressed genes specifically present in the soil strain B. mycoides SB8 in response to root exudates. Red represents significant upregulation and blue indicates significant downregulation of gene expression.

A group of genes related to metabolic processes were specifically altered in the endophytic strain EC18 (Figure 7). These included four upregulated hydrolase genes (BG05_RS07285, BG05_RS13815, BG05_RS18105, and BG05_RS20350) and one downregulated hydrolase gene (BG05_RS16815). Additionally, four genes of the alpha/beta hydrolase fold protein family were upregulated and three downregulated in EC18. In comparison, only three alpha/beta hydrolase genes are altered in SB8 (two upregulated and one downregulated) (Figure 8). Proteins of the alpha/beta hydrolase fold family are involved in the degradation of plant cell wall polymers in Burkholderia spp. (Ali et al., 2014). Similarly, the expression of pullulanase (BG05_RS18715) and of the chitin-binding protein (BG05_RS19070) was upregulated in EC18. These two genes belong to the O-glycosyl hydrolase family and it might be possible that they are involved in the metabolism of plant-derived compounds. Three proteolysis genes including pyrrolidone-carboxylate peptidase (BG05_RS16435), D-alanyl-D-alanine carboxypeptidase (BG05_RS18945), and a serine protease (BG05_RS21540) were upregulated in EC18 (Figure 7), while all three proteolysis genes were repressed in SB8. Moreover, five glyoxalase genes were specifically upregulated in EC18 (Figure 8). Five genes encoding glucose/ribitol dehydrogenase (BG05_RS12980, BG05_RS14070, BG05_RS17890, BG05_RS24540, and BG05_RS07590) were upregulated in EC18. The overall metabolic gene expression profile suggests that the endophytic strain consumes a limited numbers of sugars (glucose/ribitol), while also using more types of other carbon substrates.

Among the genes related to stress response, three genes involved in multidrug resistance are downregulated in two strains, with two genes (BG05_RS22665 and BG05_RS26910) being more significantly altered in EC18 (Figures 7, 8). A stress-induced protein (BG05_RS01935), a stress response protein (BG05_RS28400), and a general stress protein (BG05_RS26065) were specifically upregulated in endophytic strain. Genes encoding a methanol dehydrogenase (BG05_RS02305) and a streptomycin biosynthesis protein (BG05_RS25055) were also upregulated.


Endophytic bacteria have emerged as a valuable source of biocontrol agents and metabolites, which has great potential in sustainable agricultural application. However, many aspects of the interaction between endophytic bacteria and plants are unclear, e.g., the endophytic colonization process and its differences compared to non-endophytic microorganisms. This study was designed to attribute the properties specific for an endophytic B. mycoides strain or its soil counterpart. Indeed, the CLSM observation showed that the endophytic strain is a more aggressive colonizer than the soil strain at an early stage of interaction with the plant root. The endophyte competes for the colonization site with the soil strain on the root surface and eventually penetrates the internal tissue of the root. It is presumed that the response of bacteria to environmental cues enables the gene expression pattern necessary for the endophytic life cycle. Our results suggest that root exudates of potato plants have a profound impact on gene expression levels in B. mycoides and that the endophytic strain showed more active or directed responses than the soil strain. The two strains showed a different metabolic preference when they encountered root exudates. The upregulation of genes related to amino acids metabolism, several proteolytic enzymes and O-glycosyl hydrolases points toward a specific adaptation to the ecological niche and a good rhizosphere fitness of the endophytic strain. In comparison, the nutrition source for the soil isolate seems to be constrained to sugars, which might hamper its colonization ability and proliferation rate in the rhizosphere. In conclusion, this study provides new insights into the different transcriptomic adaptations between an endophytic bacterium and its soil counterpart in response to potato root exudates and provides new knowledge on endophytic bacteria-plant interactions.

Author Contributions

Conceived and designed experiments: YY, OK. Performed the experiments: YY. Performed GFP and RFP selection experiments: EF and YY. Performed bioinformatic analysis: YY and AJ. Wrote the paper: YY, OK, and EF.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.


We thank Prof. Jan Dirk van Elsas, Prof. Theo Elzinga, and Jan Spoelder at the University of Groningen for providing B. mycoides strains. YY was supported by a scholarship from China Scholarship Council.


  1. ^
  2. ^
  3. ^


Ali, S., Duan, J., Charles, T. C., and Glick, B. R. (2014). A bioinformatics approach to the determination of genes involved in endophytic behavior in Burkholderia spp. J. Theor. Biol. 343, 193–198. doi: 10.1016/j.jtbi.2013.10.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Altenhoff, A. M., Škunca, N., Glover, N., Train, C.-M., Sueki, A., Piližota, I., et al. (2014). The OMA orthology database in 2015: function predictions, better plant support, synteny view and other improvements. Nucleic Acids Res. 43, D240–D249. doi: 10.1093/nar/gku1158

PubMed Abstract | CrossRef Full Text | Google Scholar

Ambrosini, A., Stefanski, T., Lisboa, B. B., Beneduzi, A., Vargas, L. K., and Passaglia, L. M. P. (2016). Diazotrophic bacilli isolated from the sunflower rhizosphere and the potential of Bacillus mycoides B38V as biofertiliser. Ann. Appl. Biol. 168, 93–110. doi: 10.1111/aab.12245

CrossRef Full Text | Google Scholar

Aziz, R. K., Bartels, D., Best, A. A., DeJongh, M., Disz, T., Edwards, R. A., et al. (2008). The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75. doi: 10.1186/1471-2164-9-75

PubMed Abstract | CrossRef Full Text | Google Scholar

Bargabus, R., Zidack, N., Sherwood, J., and Jacobsen, B. (2002). Characterisation of systemic resistance in sugar beet elicited by a non-pathogenic, phyllosphere-colonizing Bacillus mycoides, biological control agent. Physiol. Mol. Plant Pathol. 61, 289–298. doi: 10.1006/pmpp.2003.0443

CrossRef Full Text | Google Scholar

Brencic, A., and Winans, S. C. (2005). Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria. Microbiol. Mol. Biol. Rev. 69, 155–194. doi: 10.1128/MMBR.69.1.155-194.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Compant, S., Clément, C., and Sessitsch, A. (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol. Biochem. 42, 669–678. doi: 10.1016/j.soilbio.2009.11.024

CrossRef Full Text | Google Scholar

de Jong, A., van der Meulen, S., Kuipers, O. P., and Kok, J. (2015). T-REx: transcriptome analysis webserver for RNA-seq expression data. BMC Genomics 16:663. doi: 10.1186/s12864-015-1834-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Di Franco, C., Beccari, E., Santini, T., Pisaneschi, G., and Tecce, G. (2002). Colony shape as a genetic trait in the pattern-forming Bacillus mycoides. BMC Microbiol. 2:33. doi: 10.1186/1471-2180-2-33

PubMed Abstract | CrossRef Full Text | Google Scholar

Fan, B., Carvalhais, L. C., Becker, A., Fedoseyenko, D., von Wirén, N., and Borriss, R. (2012). Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates. BMC Microbiol. 12:116. doi: 10.1186/1471-2180-12-116

PubMed Abstract | CrossRef Full Text | Google Scholar

Glick, B. R. (2012). Plant growth-promoting bacteria: mechanisms and applications. Scientifica 2012:963401. doi: 10.6064/2012/963401

PubMed Abstract | CrossRef Full Text | Google Scholar

Gómez-Lama Cabanás, C., Schilirò, E., Valverde-Corredor, A., and Mercado-Blanco, J. (2014). The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots. Front. Microbiol. 5:427. doi: 10.3389/fmicb.2014.00427

PubMed Abstract | CrossRef Full Text | Google Scholar

Hardoim, P. R., van Overbeek, L. S., and Elsas, J. D. (2008). Properties of bacterial endophytes and their proposed role in plant growth. Trends. Microbiol. 16, 463–471. doi: 10.1016/j.tim.2008.07.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Harris, M. A., Clark, J., Ireland, A., Lomax, J., Ashburner, M., Foulger, R., et al. (2004). The gene ontology (GO) database and informatics resource. Nucleic Acids Res. 32(Suppl. 1), D258–D261.

Google Scholar

Harun-Or-Rashid, M., Khan, A., Hossain, M. T., and Chung, Y. R. (2017). Induction of systemic resistance against aphids by endophytic Bacillus velezensis YC7010 via expressing PHYTOALEXIN DEFICIENT4 in Arabidopsis. Front. Plant Sci. 8:211. doi: 10.3389/fpls.2017.00211

PubMed Abstract | CrossRef Full Text | Google Scholar

Helmann, J. D., and Chamberlin, M. J. (1987). DNA sequence analysis suggests that expression of flagellar and chemotaxis genes in Escherichia coli and Salmonella typhimurium is controlled by an alternative sigma factor. Proc. Natl. Acad. Sci. U.S.A. 84, 6422–6424. doi: 10.1073/pnas.84.18.6422

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoskisson, P., Sharples, G., and Hobbs, G. (2003). The importance of amino acids as carbon sources for Micromonospora echinospora (ATCC 15837). Lett. Appl. Microbiol. 36, 268–271. doi: 10.1046/j.1472-765X.2003.01306.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Janczarek, M., Rachwał, K., Marzec, A., Grządziel, J., and Palusińska-Szysz, M. (2015). Signal molecules and cell-surface components involved in early stages of the legume–rhizobium interactions. Appl. Soil Ecol. 85, 94–113. doi: 10.1111/j.1462-5822.2010.01561.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Jasim, B., Jimtha John, C., Shimil, V., Jyothis, M., and Radhakrishnan, E. (2014). Studies on the factors modulating indole-3-acetic acid production in endophytic bacterial isolates from Piper nigrum and molecular analysis of ipdc gene. J. Appl. Microbiol. 117, 786–799. doi: 10.1111/jam.12569

PubMed Abstract | CrossRef Full Text | Google Scholar

Ji, X., Lu, G., Gai, Y., Zheng, C., and Mu, Z. (2008). Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain. FEMS Microbiol. Ecol. 65, 565–573. doi: 10.1111/j.1574-6941.2008.00543.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Jin, H., Yang, X., Yan, Z., Liu, Q., Li, X., Chen, J., et al. (2014). Characterization of rhizosphere and endophytic bacterial communities from leaves, stems and roots of medicinal Stellera chamaejasme L. Syst. Appl. Microbiol. 37, 376–385. doi: 10.1016/j.syapm.2014.05.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Kandel, S. L., Firrincieli, A., Joubert, P. M., Okubara, P., Leston, N. D., McGeorge, K. M., et al. (2017). An in vitro study of bio-control and plant growth promotion potential of Salicaceae endophytes. Front. Microbiol. 8:386. doi: 10.3389/fmicb.2017.00386

PubMed Abstract | CrossRef Full Text | Google Scholar

Kuiper, I., Bloemberg, G. V., Noreen, S., Thomas-Oates, J. E., and Lugtenberg, B. J. (2001). Increased uptake of putrescine in the rhizosphere inhibits competitive root colonization by Pseudomonas fluorescens strain WCS365. Mol. Plant Microbe Interact. 14, 1096–1104. doi: 10.1094/MPMI.2001.14.9.1096

PubMed Abstract | CrossRef Full Text | Google Scholar

Kuiper, I., Lagendijk, E. L., Bloemberg, G. V., and Lugtenberg, B. J. (2004). Rhizoremediation: a beneficial plant-microbe interaction. Mol. Plant Microbe Interact. 17, 6–15. doi: 10.1094/MPMI.2004.17.1.6

PubMed Abstract | CrossRef Full Text | Google Scholar

Lagesen, K., Hallin, P., Rødland, E. A., Stærfeldt, H.-H., Rognes, T., and Ussery, D. W. (2007). RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 35, 3100–3108. doi: 10.1093/nar/gkm160

PubMed Abstract | CrossRef Full Text | Google Scholar

Lakshmanan, V., Castaneda, R., Rudrappa, T., and Bais, H. P. (2013). Root transcriptome analysis of Arabidopsis thaliana exposed to beneficial Bacillus subtilis FB17 rhizobacteria revealed genes for bacterial recruitment and plant defense independent of malate efflux. Planta 238, 657–668. doi: 10.1007/s00425-013-1920-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, S., Flores-Encarnacion, M., Contreras-Zentella, M., Garcia-Flores, L., Escamilla, J., and Kennedy, C. (2004). Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cytochrome c biogenesis genes. J. Bacteriol. 186, 5384–5391. doi: 10.1128/JB.186.16.5384-5391.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Lowe, T. M., and Eddy, S. R. (1997). tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 25, 955–964. doi: 10.1093/nar/25.5.0955

PubMed Abstract | CrossRef Full Text | Google Scholar

Lugtenberg, B. J., Dekkers, L., and Bloemberg, G. V. (2001). Molecular determinants of rhizosphere colonization by Pseudomonas. Annu. Rev. Phytopathol. 39, 461–490. doi: 10.1146/annurev.phyto.39.1.461

PubMed Abstract | CrossRef Full Text | Google Scholar

Malfanova, N., Lugtenberg, B., and Berg, G. (2013). Bacterial Endophytes: Who and Where, and What are they Doing there? Hoboken, NJ: Wiley-Blackwell.

Google Scholar

Mark, G. L., Dow, J. M., Kiely, P. D., Higgins, H., Haynes, J., Baysse, C., et al. (2005). Transcriptome profiling of bacterial responses to root exudates identifies genes involved in microbe-plant interactions. Proc. Natl. Acad. Sci. U.S.A. 102, 17454–17459. doi: 10.1073/pnas.0506407102

PubMed Abstract | CrossRef Full Text | Google Scholar

Martinez, J. L., Sanchez, M. B., Martinez-Solano, L., Hernandez, A., Garmendia, L., Fajardo, A., et al. (2009). Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems. FEMS Microbiol. Rev. 33, 430–449. doi: 10.1111/j.1574-6976.2008.00157.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Mirel, D. B., and Chamberlin, M. J. (1989). The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase. J. Bacteriol. 171, 3095–3101. doi: 10.1128/jb.171.6.3095-3101.1989

PubMed Abstract | CrossRef Full Text | Google Scholar

Molina-Henares, A. J., Krell, T., Eugenia Guazzaroni, M., Segura, A., and Ramos, J. L. (2006). Members of the IclR family of bacterial transcriptional regulators function as activators and/or repressors. FEMS Microbiol. Rev. 30, 157–186. doi: 10.1111/j.1574-6976.2005.00008.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Mónica, R., and Esperanza, M.-R. (2006). Bacterial endophytes and their interactions with Hosts. Mol. Plant Microbe Interact. 19, 827–837. doi: 10.1094/MPMI-19-0827

PubMed Abstract | CrossRef Full Text | Google Scholar

Moreno, R., Martínez-Gomariz, M., Yuste, L., Gil, C., and Rojo, F. (2009). The Pseudomonas putida Crc global regulator controls the hierarchical assimilation of amino acids in a complete medium: evidence from proteomic and genomic analyses. Proteomics 9, 2910–2928. doi: 10.1002/pmic.200800918

PubMed Abstract | CrossRef Full Text | Google Scholar

Namy, O., Mock, M., and Fouet, A. (1999). Co-existence of clpB and clpC in the Bacillaceae. FEMS Microbiol. Lett. 173, 297–302. doi: 10.1111/j.1574-6968.1999.tb13517.x

CrossRef Full Text | Google Scholar

Neher, O. T., Johnston, M. R., Zidack, N. K., and Jacobsen, B. J. (2009). Evaluation of Bacillus mycoides isolate BmJ and B. mojavensis isolate 203-7 for the control of anthracnose of cucurbits caused by Glomerella cingulata var. orbiculare. Biol. Control 48, 140–146. doi: 10.1016/j.biocontrol.2008.08.012

CrossRef Full Text | Google Scholar

Pangesti, N., Pineda, A., Dicke, M., and Loon, J. (2015). Variation in plant-mediated interactions between rhizobacteria and caterpillars: potential role of soil composition. Plant Biol. 17, 474–483. doi: 10.1111/plb.12265

PubMed Abstract | CrossRef Full Text | Google Scholar

Phillips, D. A., Joseph, C. M., and Maxwell, C. A. (1992). Trigonelline and stachydrine released from alfalfa seeds activate NodD2 protein in Rhizobium meliloti. Plant Physiol. 99, 1526–1531. doi: 10.1104/pp.99.4.1526

PubMed Abstract | CrossRef Full Text | Google Scholar

Prashar, P., Kapoor, N., and Sachdeva, S. (2013). Rhizosphere: its structure, bacterial diversity and significance. Rev. Environ. Sci. Bio/Technol. 13, 63–77. doi: 10.1007/s11157-013-9317-z

CrossRef Full Text | Google Scholar

Prieto, P., Schiliro, E., Maldonado-Gonzalez, M. M., Valderrama, R., Barroso-Albarracin, J. B., and Mercado-Blanco, J. (2011). Root hairs play a key role in the endophytic colonization of olive roots by Pseudomonas spp. with biocontrol activity. Microb. Ecol. 62, 435–445. doi: 10.1007/s00248-011-9827-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Rosconi, F., Davyt, D., Martínez, V., Martínez, M., Abin-Carriquiry, J. A., Zane, H., et al. (2013). Identification and structural characterization of serobactins, a suite of lipopeptide siderophores produced by the grass endophyte Herbaspirillum seropedicae. Environ. Microbiol. 15, 916–927. doi: 10.1111/1462-2920.12075

PubMed Abstract | CrossRef Full Text | Google Scholar

Rosenblueth, M., and Martínez-Romero, E. (2004). Rhizobium etli maize populations and their competitiveness for root colonization. Arch. Microbiol. 181, 337–344. doi: 10.1007/s00203-004-0661-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Santos, F., Peñaflor, M. F. G., Paré, P. W., Sanches, P. A., Kamiya, A. C., Tonelli, M., et al. (2014). A novel interaction between plant-beneficial rhizobacteria and roots: colonization induces corn resistance against the root herbivore Diabrotica speciosa. PLoS ONE 9:e113280. doi: 10.1371/journal.pone.0113280

PubMed Abstract | CrossRef Full Text | Google Scholar

Santoyo, G., Moreno-Hagelsieb, G., Orozco-Mosqueda Mdel, C., and Glick, B. R. (2016). Plant growth-promoting bacterial endophytes. Microbiol. Res. 183, 92–99. doi: 10.1016/j.micres.2015.11.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Schaefer, A. L., Oda, Y., Coutinho, B. G., Pelletier, D. A., Weiburg, J., Venturi, V., et al. (2016). A LuxR homolog in a cottonwood tree endophyte that activates gene expression in response to a plant signal or specific peptides. mBio 7:e01101-16. doi: 10.1128/mBio.01101-16

PubMed Abstract | CrossRef Full Text | Google Scholar

Shidore, T., Dinse, T., Ohrlein, J., Becker, A., and Reinhold-Hurek, B. (2012). Transcriptomic analysis of responses to exudates reveal genes required for rhizosphere competence of the endophyte Azoarcus sp. strain BH72. Environ. Microbiol. 14, 2775–2787. doi: 10.1111/j.1462-2920.2012.02777.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Siguier, P., Pérochon, J., Lestrade, L., Mahillon, J., and Chandler, M. (2006). ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res. 34(Suppl. 1), D32–D36. doi: 10.1093/nar/gkj014

PubMed Abstract | CrossRef Full Text | Google Scholar

Song, Y., Tong, Z., Wang, J., Wang, L., Guo, Z., Han, Y., et al. (2004). Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans. DNA Res. 11, 179–197. doi: 10.1093/dnares/11.3.179

PubMed Abstract | CrossRef Full Text | Google Scholar

Taghavi, S., Wu, X., Ouyang, L., Zhang, Y. B., Stadler, A., McCorkle, S., et al. (2015). Transcriptional responses to sucrose mimic the plant-associated lifestyle of the plant growth promoting endophyte Enterobacter sp. 638. PLoS ONE 10:e0115455. doi: 10.1371/journal.pone.0115455

PubMed Abstract | CrossRef Full Text | Google Scholar

Tatusov, R. L., Fedorova, N. D., Jackson, J. D., Jacobs, A. R., Kiryutin, B., Koonin, E. V., et al. (2003). The COG database: an updated version includes eukaryotes. BMC Bioinformatics 4:41. doi: 10.1186/1471-2105-4-41

PubMed Abstract | CrossRef Full Text | Google Scholar

Timm, C. M., Campbell, A. G., Utturkar, S. M., Jun, S. R., Parales, R. E., Tan, W. A., et al. (2015). Metabolic functions of Pseudomonas fluorescens strains from Populus deltoides depend on rhizosphere or endosphere isolation compartment. Front. Microbiol. 6:1118. doi: 10.3389/fmicb.2015.01118

CrossRef Full Text | Google Scholar

van Peer, R., Punte, H. L., de Weger, L. A., and Schippers, B. (1990). Characterization of root surface and endorhizosphere pseudomonads in relation to their colonization of roots. Appl. Environ. Microbiol. 56, 2462–2470.

PubMed Abstract | Google Scholar

Wakelin, S. A., Warren, R. A., Harvey, P. R., and Ryder, M. H. (2004). Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biol. Fertil. Soils 40, 36–43. doi: 10.1007/s00374-004-0750-6

CrossRef Full Text | Google Scholar

Wilson, A. C., Perego, M., and Hoch, J. A. (2007). New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis. J. Microbiol. Methods 71, 332–335. doi: 10.1016/j.mimet.2007.09.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Xie, S., Wu, H., Chen, L., Zang, H., Xie, Y., and Gao, X. (2015). Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings. BMC Microbiol. 15:21. doi: 10.1186/s12866-015-0353-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Yi, H.-S., Yang, J. W., and Ryu, C.-M. (2013). ISR meets SAR outside: additive action of the endophyte Bacillus pumilus INR7 and the chemical inducer, benzothiadiazole, on induced resistance against bacterial spot in field-grown pepper. Front. Plant Sci. 4:122. doi: 10.3389/fpls.2013.00122

PubMed Abstract | CrossRef Full Text | Google Scholar

Yi, Y., and Kuipers, O. P. (2017). Development of an efficient electroporation method for rhizobacterial Bacillus mycoides strains. J. Microbiol. Methods 133, 82–86. doi: 10.1016/j.mimet.2016.12.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Zerbino, D. R., and Birney, E. (2008). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 18, 821–829. doi: 10.1101/gr.074492.107

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, N., Wang, D., Liu, Y., Li, S., Shen, Q., and Zhang, R. (2014). Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere-associated bacterial strains. Plant Soil 374, 689–700. doi: 10.1007/s11104-013-1915-6

CrossRef Full Text | Google Scholar

Zhang, N., Yang, D., Wang, D., Miao, Y., Shao, J., Zhou, X., et al. (2015). Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 during enhanced biofilm formation regulated by maize root exudates. BMC Genomics 16:685. doi: 10.1186/s12864-015-1825-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, Y., Liang, Y., Lynch, K. H., Dennis, J. J., and Wishart, D. S. (2011). PHAST: a fast phage search tool. Nucleic Acids Res. 39(Suppl. 2), W347–W352. doi: 10.1093/nar/gkr485

PubMed Abstract | CrossRef Full Text | Google Scholar

Zinser, E. R., and Kolter, R. (1999). Mutations enhancing amino acid catabolism confer a growth advantage in stationary phase. J. Bacteriol. 181, 5800–5807.

PubMed Abstract | Google Scholar

Keywords: Bacillus mycoides, endophyte, root colonization, transcriptome, root exudates

Citation: Yi Y, de Jong A, Frenzel E and Kuipers OP (2017) Comparative Transcriptomics of Bacillus mycoides Strains in Response to Potato-Root Exudates Reveals Different Genetic Adaptation of Endophytic and Soil Isolates. Front. Microbiol. 8:1487. doi: 10.3389/fmicb.2017.01487

Received: 25 May 2017; Accepted: 24 July 2017;
Published: 04 August 2017.

Edited by:

Yunrong Chai, Northeastern University, United States

Reviewed by:

Collin M. Timm, Applied Physics Laboratory, Johns Hopkins University, United States
Munusamy Madhaiyan, Temasek Life Sciences Laboratory, Singapore

Copyright © 2017 Yi, de Jong, Frenzel and Kuipers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Oscar P. Kuipers,

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.