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Front. Microbiol., 05 October 2023
Sec. Microbial Symbioses
This article is part of the Research Topic Symbiotic Interactions of Algae and Microorganisms: Physiology and Industrial Applications View all 5 articles

Temporal force governs the microbial assembly associated with Ulva fasciata (Chlorophyta) from an integrated multi-trophic aquaculture system

\r\nDzung Nguyen,Dzung Nguyen1,2Ofer OvadiaOfer Ovadia3Lior Guttman,
Lior Guttman2,3*
  • 1Marine Biology and Biotechnology Program, Department of Life Sciences, Ben-Gurion University of the Negev, Eilat, Israel
  • 2Israel Oceanographic and Limnological Research, The National Center for Mariculture, Eilat, Israel
  • 3Department of Life Sciences, Ben-Gurion University of the Negev, Be'er Sheva, Israel

Ulva spp., one of the most important providers of marine ecosystem services, has gained substantial attention lately in both ecological and applicational aspects. It is known that macroalgae and their associated microbial community form an inseparable unit whose intimate relationship can affect the wellbeing of both. Different cultivation systems, such as integrated multi-trophic aquaculture (IMTA), are assumed to impact Ulva bacterial community significantly in terms of compositional guilds. However, in such a highly dynamic environment, it is crucial to determine how the community dynamics change over time. In the current study, we characterized the microbiota associated with Ulva fasciata grown as a biofilter in an IMTA system in the Gulf of Aqaba (Eilat, Israel) over a developmental period of 5 weeks. The Ulva-associated microbial community was identified using the 16S rRNA gene amplicon sequencing technique, and ecological indices were further analyzed. The Ulva-associated microbiome revealed a swift change in composition along the temporal succession, with clusters of distinct communities for each timepoint. Proteobacteria, Bacteroidetes, Planctomycetes, and Deinococcus-Thermus, the most abundant phyla that accounted for up to 95% of all the amplicon sequence variants (ASVs) found, appeared in all weeks. Further analyses highlighted microbial biomarkers representing each timepoint and their characteristics. Finally, the presence of highly abundant species in Ulva microbiota yet underestimated in previous research (such as phyla Deinococcus-Thermus, families Saprospiraceae, Thiohalorhabdaceae, and Pirellulaceae) suggests that more attention should be paid to the temporal succession of the assembly of microbes inhabiting macroalgae in aquaculture, in general, and IMTA, in particular. Characterizing bacterial communities associated with Ulva fasciata from an IMTA system provided a better understanding of their associated microbial dynamics and revealed this macroalgae's adaptation to such a habitat.

1. Introduction

Ulva fasciata, a species belonging to the Ulva green macroalgae genus, plays a pivotal role in brackish and marine ecosystems as ecosystem engineers (Lobban and Wynne, 1981). Like other marine eukaryotes such as coral (Rosenberg et al., 2007; Bourne et al., 2009) and marine invertebrates (Dubilier et al., 2008), macroalgae as a group of photosynthetic sessile organisms (Florez et al., 2017) also maintain a stable intimate relationship with their microbiota to form a consolidated system (Egan et al., 2013). Associated bacteria can provide macroalgae with vital nutrients and vitamins for normal growth (Provasoli and Pintner, 1980; Croft et al., 2005), contribute to nitrogen fixation (Penhale and Capone, 1981), inhibit undesirable colonies, and detach biofouling species from the seaweed (Dobretsov et al., 2006; Egan et al., 2008; Wiese et al., 2009; Ismail et al., 2016). In macroalgal species such as Ulva fasciata (Singh et al., 2011) and others [Ulva linza, Ulva compressa (Fries, 1975), and Ulva pertusa (Nakanishi et al., 1999)], epiphytic bacteria are known to play a substantial role in their morphological development. From a mutually beneficial perspective, seaweeds are dependent on the associated microbiome to complement their functions while they, in turn, provide essential niches and resources that can assist the microbial community settlement (van der Loos et al., 2019).

Given the importance of the U. fasciata-associated microbiota, it is important to note that specific environments and conditions can determine its resident bacteria, leading to a selection of different microbial compositional guilds and directly impacting seaweed physiology (van der Loos et al., 2019). Integrated multi-trophic aquaculture (IMTA) refers to a system where fed and extractive species are grown together to increase the recovery of nutrient residues and hence decrease the environmental footprints of aquaculture activities (Neori et al., 2019). It was suggested that the high abundance of the class Alphaproteobacteria in aquaculture habitats may contribute to the growth of Ulva (Califano et al., 2020). However, due to current limited research on macroalgae in IMTA systems, further experiment-based studies are required to corroborate this knowledge. In Israel, Ulva has been utilized as a crucial part of the IMTA biofiltration system for several decades due to its capability to assimilate excess nutrients (most importantly ammonia) from fish farm effluents (Shpigel et al., 2019; Shahar and Guttman, 2020). A study on macroalgal bacteria (Califano et al., 2020) revealed that an IMTA caused changes in the prokaryotic community associated with Ulva compared with that from the wild. Though this was among the important pioneer studies on Ulva microbiota in an IMTA setting, there remains a gap in monitoring the microbial community along the temporal dimension under the same culture mode. Temporal dynamics are expected to play a crucial part in shaping the microbial assembly in algal thalli and thereby constitute a stepping stone for more studies regarding macroalgae and microbial symbioses. Generally, very few studies have tried to characterize the U. fasciata-associated microbial community, especially in an IMTA environment. Given that Ulva spp. have become a universally popular component of the IMTA system, it becomes relevant to study their associated microbiota for ecological and practical purposes.

Based on this background, our research aimed at the characterization of the microbial community associated with U. fasciata during a period of 5 weeks under an IMTA setup. The results will not only contribute to the knowledge gap about the taxonomic diversity of the algal microbiome in an IMTA system but also provide insights into the temporal dynamics of this community in specific conditions for further application.

2. Materials and methods

2.1. Experiment setup

The experiment took place for 5 weeks in March–April 2020. The system was designed following a previous study where U. fasciata was integrated as a biofilter at the Israel Oceanographic and Limnological Research—The National Center for Mariculture (IOLR-NCM) in the Gulf of Aqaba (Eilat, Israel) (Shahar and Guttman, 2020). Seawater was pumped to fish tanks from a location of ~300 m offshore (32°29′N and 58°34′E) from a depth of 13 meters (Nguyen et al., 2023). Following the original concept of IMTA, three fishponds (40 m3 each) of gray mullet (Mugil cephalus) were located upstream of the Ulva-based biofilter (Figure 1), from which the effluent water was fed into the Ulva cultivation tanks. The flow rate of input seawater and effluent discharge was ~4 m3 d−1. Additional nutrients that support the optimal performance of the seaweed were supplied from a 250 L tank through a dosing pump (VMS 2001, EMEC, Italy), resulting in a volume that comprised 10% of the total flow from the Ulva's inlet water source. Nutrient concentrations of the cultivation water were measured during the experimental period, including TAN-N, NO3-N, and PO4-P with respective inlet concentrations of 1.51 ± 0.47, 0.92 ± 0.76, and 0.19 ± 0.09 mg L−1 and outlet concentrations of 1.24 ± 0.70, 0.88 ± 0.69, and 0.18 ± 0.12 mg L−1 (Supplementary Table 1A). In addition, additional relevant physical parameters were also measured throughout the experiment, such as temperature (20.84 ± 1.6°C), dissolved oxygen (7.91 ± 0.52 mg/L or 113.33% ± 6.93%), and pH (7.99 ± 0.05) (Supplementary Table 1B). At the beginning of the experiment, 1 kg of fresh Ulva fasciata was collected from a production tank at IOLR-NCM and transferred to the experimental tanks holding a volume of 550 L each. An aeration system was installed at the bottom of each tank to control the concentration of oxygen and ensure that algae were kept circulating in the water column (Guttman et al., 2018). The macroalgae were harvested on a weekly basis so that only 1 kg of U. fasciata would be retained for development.


Figure 1. Experimental setup of the U. fasciata-based biofilter system. Effluents from fish tanks were routed to the sedimentation pond and enriched with nutrients, then pumped to the biofilter system consisting of three biological replicates.

2.2. U. fasciata sample collection

Algal thalli were collected once a week and distributed into different samples as water brings the thalli to the upper part of the tank during aeration. Five technical replicates (~20 g each) consisting of three thalli each were harvested from each tank, stored in sterile plastic bags filled with water from the sample tank, placed in an icebox, and transported to the laboratory (Penesyan et al., 2009; Ismail et al., 2018; Califano et al., 2020). In the laboratory, under sterile conditions, we chose only three healthy samples having green thalli from each tank for further analyses. Sessile organisms on the surface of algal thallus were checked carefully and, if present, were carefully removed with a sterile scraper (Califano et al., 2020). The algal samples were, then, washed three times, filtered, and autoclaved natural seawater to remove loosely associated bacteria (Jiang et al., 1999; Burgess et al., 2003). After the checkup, the samples were stored at −80°C until DNA extraction (Califano et al., 2020).

2.3. DNA extraction and amplification

From each sample, an approximate biomass of 200 g of algal thalli was subsampled and cut into smaller pieces for DNA extraction. The algal DNA was extracted using the PureLinkTM Microbiome DNA Purification Kit (Thermo Fisher Scientific) following the manufacturer's protocol. The bacterial community was identified using the primer set 515 Fa/926 R, targeting the V4–V5 hypervariable region of the 16S rRNA gene as recommended by the Earth Microbiome Project (Caporaso et al., 2012; Walters et al., 2016). The PCR performance included initial denaturation at 95°C for 5 min, 28 cycles of 94°C for 45 s, 50°C for 60 s, 72°C for 90 s, and final elongation at 72°C for 10 min. PCR products were analyzed via gel electrophoresis in 2.0% agarose and then stored at −20°C until sequencing (

2.4. Amplicon sequencing and data processing

Sequencing was performed on an Illumina MiSeq at Chicago Research Informatics Core (University of Illinois). The merging of forward and reverse reads was conducted by Paired-End Read Merger (PEAR) (Zhang et al., 2014), after which adapters, primers, and ambiguous nucleotides were discarded from the reads. Chimeric sequences were identified using the USEARCH algorithm (Alloui et al., 2015) as compared with a reference database. Amplicon sequence variants (ASVs) were picked up using deficiency of adenosine deaminase 2 (DADA2) at a 97% similarity threshold, while the taxonomy was annotated via the Naive Bayesian approach under the DADA2 package for bacteria (Callahan et al., 2016). Taxonomy assignment for prokaryotes (bacteria and archaea) was, then, completed by using the Silva database (Quast et al., 2012) as a reference. Five samples that had a very low number of sequences (<150 counts/sample) were discarded from the whole dataset; therefore, in the end, only 40 samples were further analyzed during the 5-week experiment (Supplementary Table 2A). Filtration was performed to exclude chloroplast and mitochondria from the samples, which culminated in an average of 53.98% retained sequences in all samples (Supplementary Table 2B). A final clean-up step was conducted to remove 334 low-count ASVs that were present in <10% of the samples for the downstream analyses.

2.5. Statistical analyses

All samples were rarefied at the minimum library size of 10,000 reads. Based on the Shannon index, alpha diversity was calculated for all samples; then, statistical test of Kruskal–Wallis was used to measure the significant difference between the diversity of samples along 5-week timepoints. Beta diversity of the community was determined by the statistical tests of permutational multivariate analysis of variance (PERMANOVA) based on the Bray–Curtis metrics and then subjected to ordination by the principal coordinate analysis (PCoA). Statistical tests were performed in R version 4.2.0 (R Development Core Team, 2010) using the vegan package version 2.6-2 (Oksanen et al., 2022). Ecological indices were calculated, and figures were created using the web-based tool MicrobiomeAnalyst (Chong et al., 2020). The linear discriminant analysis (LDA) effect size (LEfSe) method was utilized to identify time biomarkers—the features (ASVs) that mostly explained the differences between samples originating from different weeks (Segata et al., 2011). In this method, the non-parametric Kruskal–Wallis rank-sum test was first used, followed by the LDA to examine the effect size of the significant features (Chong et al., 2020).

3. Results

3.1. Composition of the microbial community associated with U. fasciata

There were 2,421,985 reads of prokaryotes sequenced from the samples, with an average of 60,549 reads per sample. A total of 911 ASVs were identified in the microbial communities associated with U. fasciata samples during 5 weeks of maturation, including 11 bacterial phyla. Four of these bacterial phyla accounted for up to >95% of the total sequence abundance in the following decreasing order: Proteobacteria, Bacteroidetes, Planctomycetes, and Deinococcus-Thermus (Figure 2A and Supplementary Table 3). Bacteria belonging to phylum Proteobacteria were enriched along the temporal development of the microbial community on Ulva, while Bacteroidetes were more abundant during weeks 2 and 3. Planctomycetes were consistently present along the 5 weeks while Deinococcus-Thermus predominated the initial successional stage. At the class level, the most prevalent classes which were present in over 10% of all samples were Bacteroidia (27.16%), Alphaproteobacteria (23.24%), Gammaproteobacteria (22.75%), and Planctomycetacia (15.69%) (Figure 2B and Supplementary Table 4). While Bacteroidia showed a decreasing trend toward the last 2 weeks of succession, both Alphaproteobacteria and Gammaproteobacteria shared the same increasing tendency throughout 5 weeks (Supplementary Table 4). At the family level, the most dominant taxa were Saprospiraceae (19.77%), Thiohalorhabdaceae (19.32%), and Pirellulaceae (13.60%) (Supplementary Figure 1 and Supplementary Table 5), while at the genus level, the most highly annotated taxa included Granulosicoccus (19.32%), Blastopirellula (11.23%), and Erythrobacter (7.28%) (Supplementary Figure 2 and Supplementary Table 6).


Figure 2. Taxonomic composition performed as the relative abundance of the bacteria associated with U. fasciata at (A) phylum and (B) class levels across 5 weeks of cultivation.

3.2. Alpha diversity of the microbial community associated with U. fasciata

Diversity of the microbial community was characterized during a 5-week cultivation period of Ulva fasciata under the IMTA setup. The Shannon index was used to measure the diversity of the microbial community associated with U. fasciata. It showed considerable changes in the community diversity, with significant differences evident between the weekly samples (Figure 3, Kruskal–Wallis test, H value = 29.63, p-value < 0.001). The highest Shannon value was in the second timepoint with an average of 4.3, while the lowest value identified was at the last timepoint with a diversity index of ~3.3. The microbial community revealed the broadest variability in terms of diversity value in the first week compared with other samples from other timepoints.


Figure 3. Changes in the bacterial community associated with Ulva during 5 weeks of temporal succession were shown by Shannon diversity; statistical Kruskal–Wallis test, H-value = 29.63, p-value < 0.001. The test was performed at the feature level; box plots show a 95% confidence interval, bar plots represent the standard error, straight lines represent the median, and black dots show the average values, n = 40.

3.3. Beta diversity of the bacterial community

The beta diversity between five microbial communities associated with U. fasciata was calculated based on Bray–Curtis metrics. The statistical test of PERMANOVA demonstrated significant clusters according to the temporal origin of the samples (Figure 4, F-value = 14.12, R-squared = 0.62, p-value < 0.001). When subjected to PCoA, the first and second axes explained up to 56.8% of the total variation across samples. Along the most explanatory axis, the first- and second-week communities tended to separate the farthest from the remaining communities. This result was evidently supported by the temporal cluster, forming distinct communities along the 5-week succession (Figure 5). As the community swiftly changed along developmental stages, each week was represented by the presence of different predominating taxonomic groups.


Figure 4. Dissimilarities in Ulva-associated microbial community during 5-week assembly performed by beta diversity at the feature level. Statistical test PERMANOVA based on Bray–Curtis metrics, then subjected to ordination on PCoA (F-value = 14.12, R-squared = 0.62, p-value < 0.001, n = 40).


Figure 5. Clustered heatmap shows taxonomic abundance variance following temporal assemblies (weeks 1 to 5) for Ulva-associated bacteria community. The graph is shown at the genus level, with red color representing more abundant taxa and blue color indicating the less abundant ones.

3.4. Biomarkers of the microbial community

Differences in microbial assemblies along the temporal succession also resulted in 22 taxa that were identified as time biomarkers via the linear discriminant analysis (LDA) with p-value < 0.05 and LDA score > 2.0. In this study, time biomarkers are ASVs whose relative abundances differ significantly between samples that come from different timepoints. The highest number of biomarking microbes (8 features) belonged to the last week of community development, followed by the first week (6 features) and second week (4 features), while weeks 3 and 4 shared the same number of biomarkers (2 features) (Figure 6). Despite temporal dynamics, the following families always played an important part as biomarkers: Saprospiraceae, Thiohalorhabdaceae, Sphingomonadaceae, and Rhodobacteraceae (Table 1).


Table 1. List of biomarkers identified during a 5-week period. Bacteria were annotated at the lowest level of the genus.


Figure 6. Top 22 bacteria identified as biomarkers in different weeks via linear discriminant analysis (LDA); significant taxa were ranked in decreasing order and defined at p-value < 0.05 and LDA score > 2.0. The heatmap on the right side shows whether the abundance of the taxa is high (red) or low (blue).

4. Discussion

Macroalgae are known to form an inseparable relationship with their associated microbiome, which can possibly impact the seaweed's functioning and ecological services, given different conditions (Ren et al., 2022). To better understand the effect of temporal force on microbial assembly in macroalgae-associated microbiota, our study characterized the succession of an Ulva fasciata-associated microbial community from an IMTA biofilter system across 5 weeks of cultivation. Ecological indices were utilized to provide insights into the temporal changes of the microbiota, with a focus on biomarker species at each timepoint.

Previous studies indicated that IMTA conditions substantially influenced the structure and composition of Ulva-associated microbiota (Califano et al., 2020; Wichard, 2022). The most abundant phyla found in our study, such as Proteobacteria, Bacteroidetes, and Planctomycetes, were in strong agreement with other studies across various locations, especially in ITMA setups (Califano et al., 2020; Wiegand et al., 2021). Proteobacteria and Bacteroidetes were classified as two major groups of primary colonizers as many microbes belonging to these phyla are known to have a positive effect on seaweed growth and development (Wiegand et al., 2021; Ren et al., 2022). On the other hand, Planctomycetes played an essential role in the biofilm complex in a wide range of macroalgae due to their antibiotic resistance and dependence on sulfated polysaccharides supplied by algae (Wiegand et al., 2021). However, Deinococcus-Thermus has not been detected before in macroalgae from IMTA systems, although species in this taxon are resistant to extreme environments such as radioactive or thermophilic conditions (Ho et al., 2016). At the class level, our results agree with the prior study in the context of IMTA, where Bacteroidia, Alpha- and Gamma- proteobacteria, and Planctomycetacia were reported as among the most abundant taxa associated with macroalgae Ulva (Califano et al., 2020). At the family level, although Saprospiraceae was generally noticed to be prevalent in planktonic water and Ulva microbiome (Burke et al., 2011; Califano et al., 2020), it was characterized by a very low abundance of 4% in the aquaculture environment (Califano et al., 2020). In our study, however, this family was present in cultivated Ulva with the highest relative abundance of almost 20%.

Moreover, we also discovered other predominant families such as Thiohalorhabdaceae and Pirellulaceae which were not reported in other research studies about macroalgae originating from an IMTA environment. Being ubiquitous in hypersaline habitats, species belonging to the Thiohalorhabdaceae family (Proteobacteria phylum) can survive various extreme conditions due to their metabolic diversity, especially by sulfur-oxidizing using oxygen or nitrate as an electron acceptor (Sorokin and Merkel, 2023). Their success in the Ulva microbiome in our IMTA facility could be partly explained due to the high salinity of the Gulf of Aqaba ambient seawater, which is approximately 41% during winter-spring time (Al-Taani et al., 2020). While Pirellulaceae (Planctomycetes phylum) may appear in a broad range of brackish and marine habitats (Lage et al., 2015), their high abundance in marine macroalgae is suggested to be due to their ability to degrade sulfated polysaccharides produced by seaweeds and microalgae (Bengtsson and Øvreås, 2010).

We further identified the time biomarkers with potential properties and functions that encouraged their survival and high prevalence in Ulva microbiota. Species from the genus Blastopirellula (dominating phylum Planctomycetes), which were biomarkers in the first and second weeks, are popularly identified as macroalgae-associated taxa, notwithstanding geographical location (Lage and Bondoso, 2014; Califano et al., 2020; Wiegand et al., 2021). In week 4, the genus Erythrobacter (Alphaproteobacteria class), which is important and widespread in the ocean environment, was found to be a biomarker. The presence of extracellular quorum sensing signals in a cultivated candidate of this genus was believed to support the biofilm formation, thereby supporting their survival on the surface of macroalgae (Abdul Malik et al., 2020). In the last week, a biomarker of the genus Granulosicoccus, found in many marine regions and especially associated with diverse marine macroalgae, appeared. Full genome sequencing of a Granulosicoccus strain revealed certain genes responsible for the lyase activity of algae polysaccharides which could support their existence in algae (Kang et al., 2018).

Regarding microbial community dynamics, swift changes were found to occur in the U. fasciata-associated microbiota structure. This aspect of the microbial community in algae cultivated from an IMTA system has not been discussed previously. The intrinsic dynamic property of the bacterial community encourages new insight into microbial development in which a rapid change in the microbiota can facilitate better understanding and bioengineering of green macroalgae. The dynamics of microbial composition during a short period of time suggest that a finer time resolution should be considered when studying Ulva-associated microbiota in the context of an IMTA system, where considerably swift changes along its coastal area could be a crucial factor in determining community assembly.

5. Conclusion

The current study contributed to knowledge about the microbial community associated with the macroalgae Ulva fasciata cultivated in an IMTA system in the Gulf of Aqaba (Eilat, Israel). The Ulva-associated microbiome exhibited high dynamics in its composition and structure along the temporal succession, with the grouping of distinct communities at each timepoint. We suggest that future research should cover longer time series within various conditions and integrate the relationship between nutrient availability and microbial assembly to fully understand the ecological patterns and underlying forces governing the microbiota. Our findings of underestimated taxa (phylum Deinococcus-Thermus, families Saprospiraceae, Thiohalorhabdaceae, and Pirellulaceae) associated with Ulva in IMTA-specific conditions suggest that more attention should be given to this microbiota in such a unique habitat and that common technical issues in omics studies (the choice of primers, sequencing techniques, and bioinformatics workflows) should be taken into account during data mining and interpretation.

Data availability statement

The dataset presented in this study can be found in the ENA online repository under the accession number PRJEB62134. Supplementary data and documents are provided in the article/Supplementary material.

Author contributions

LG, OO, and DN: conceptualization and writing. DN and LG: investigation, interpretation of results, visualization, and editing. LG: funding acquisition, project administration, and supervision. All authors read and approved the final manuscript.


This study was funded by the US-Israel Binational Agricultural Research and Development Fund (BARD), Grant Nos. 4995-17R and IS-5479-22C.


The authors would like to thank the local staff at IOLR-NCM, M. Masasa, R. Barkan, I. Hurtwiz, and H. Chernova for all their technical support, and Mikhal Ben-Shaprut for English editing.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at:


ASV, amplicon sequence variant; DADA2, deficiency of adenosine deaminase 2; IOLR-NCM, Israel Oceanographic and Limnological Research—National Center for Mariculture, Israel; LDA, linear discriminant analysis; PCoA, principal coordinate analysis; PEAR, paired-end read merger; PERMANOVA, permutational multivariate analysis of variance.


Abdul Malik, S. A., Bazire, A., Gamboa-Muñoz, A., Bedoux, G., Robledo, D., García-Maldonado, J. Q., et al. (2020). Screening of surface-associated bacteria from the Mexican red alga Halymenia floresii for quorum sensing activity. Microbiology 89, 778–788. doi: 10.1134/S0026261720060132

CrossRef Full Text | Google Scholar

Alloui, T., Boussebough, I., Chaoui, A., Nouar, A. Z., and Chettah, M. C. (2015). “Usearch: a meta search engine based on a new result merging strategy,” in 2015 7th International Joint Conference on Knowledge Discovery, Knowledge Engineering and Knowledge Management (IC3K) (Lisbon: IEEE), 531–536.

Google Scholar

Al-Taani, A. A., Rashdan, M., Nazzal, Y., Howari, F., Iqbal, J., Al-Rawabdeh, A., et al. (2020). Evaluation of the Gulf of Aqaba coastal water, Jordan. Water 12, 2125. doi: 10.3390/w12082125

CrossRef Full Text | Google Scholar

Bengtsson, M. M., and Øvreås, L. (2010). Planctomycetes dominate biofilms on surfaces of the kelp Laminaria hyperborea. BMC Microbiol. 10, 1–12. doi: 10.1186/1471-2180-10-261

PubMed Abstract | CrossRef Full Text | Google Scholar

Bourne, D. G., Garren, M., Work, T. M., Rosenberg, E., Smith, G. W., and Harvell, C. D. (2009). Microbial disease and the coral holobiont. Trends Microbiol. 17, 554–562. doi: 10.1016/j.tim.09.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Burgess, J. G., Boyd, K. G., Armstrong, E., Jiang, Z., Yan, L., Berggren, M., et al. (2003). The development of a marine natural product-based antifouling paint. Biofouling 19, 197–205. doi: 10.1080/0892701031000061778

PubMed Abstract | CrossRef Full Text | Google Scholar

Burke, C., Thomas, T., Lewis, M., Steinberg, P., and Kjelleberg, S. (2011). Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis. ISME J. 5, 590–600. doi: 10.1038/ismej.2010.164

PubMed Abstract | CrossRef Full Text | Google Scholar

Califano, G., Kwantes, M., Abreu, M. H., Costa, R., and Wichard, T. (2020). Cultivating the macroalgal holobiont: effects of integrated multi-trophic aquaculture on the microbiome of Ulva rigida (Chlorophyta). Front. Mar. Sci. 7, 52. doi: 10.3389/fmars.2020.00052

CrossRef Full Text | Google Scholar

Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., and Holmes, S. P. (2016). DADA2: high-resolution sample inference from Illumina amplicon data. Nat. Methods 13, 581–583. doi: 10.1038/nmeth.3869

PubMed Abstract | CrossRef Full Text | Google Scholar

Caporaso, J. G., Lauber, C. L., Walters, W. A., Berg-Lyons, D., Huntley, J., Fierer, N., et al. (2012). Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6, 1621–1624. doi: 10.1038/ismej.2012.8

PubMed Abstract | CrossRef Full Text | Google Scholar

Chong, J., Liu, P., Zhou, G., and Xia, J. (2020). Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data. Nat. Protoc. 15, 799–821. doi: 10.1038/s41596-019-0264-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Croft, M. T., Lawrence, A. D., Raux-Deery, E., Warren, M. J., and Smith, A. G. (2005). Algae acquire vitamin B12 through a symbiotic relationship with bacteria. Nature 438, 90–93. doi: 10.1038/nature04056

PubMed Abstract | CrossRef Full Text | Google Scholar

Dobretsov, S., Dahms, H. U., and Qian, P. Y. (2006). Inhibition of biofouling by marine microorganisms and their metabolites. Biofouling 22, 43–54. doi: 10.1080/08927010500504784

PubMed Abstract | CrossRef Full Text | Google Scholar

Dubilier, N., Bergin, C., and Lott, C. (2008). Symbiotic diversity in marine animals: the art of harnessing chemosynthesis. Nat. Rev. Microbiol. 6, 725–740. doi: 10.1038/nrmicro1992

PubMed Abstract | CrossRef Full Text | Google Scholar

Egan, S., Harder, T., Burke, C., Steinberg, P., Kjelleberg, S., and Thomas, T. (2013). The seaweed holobiont: understanding seaweed–bacteria interactions. FEMS Microbiol. Rev. 37, 462–476. doi: 10.1111/1574-6976.12011

CrossRef Full Text | Google Scholar

Egan, S., Thomas, T., and Kjelleberg, S. (2008). Unlocking the diversity and biotechnological potential of marine surface associated microbial communities. COMICR 11, 219–225. doi: 10.1016/j.mib.04001

PubMed Abstract | CrossRef Full Text | Google Scholar

Florez, J. Z., Camus, C., Hengst, M. B., and Buschmann, A. H. (2017). A functional perspective analysis of macroalgae and epiphytic bacterial community interaction. Front. Microbiol. 8, 2561. doi: 10.3389/fmicb.2017.02561

PubMed Abstract | CrossRef Full Text | Google Scholar

Fries, L. (1975). Some observations on morphology of enteromorpha-linza (l) j-ag and enteromorpha-compressa (l) grev in axenic culture. Bot. Mar. 18, 251–253.

Google Scholar

Guttman, L., Boxman, S. E., Barkan, R., Neori, A., and Shpigel, M. (2018). Combinations of Ulva and periphyton as biofilters for both ammonia and nitrate in mariculture fishpond effluents. Algal Res. 34, 235–243. doi: 10.1016/j.algal.08002

CrossRef Full Text | Google Scholar

Ho, J., Adeolu, M., Khadka, B., and Gupta, R. S. (2016). Identification of distinctive molecular traits that are characteristic of the phylum “Deinococcus-Thermus” and distinguish its main constituent groups. Syst. Appl. Microbiol. 39, 453–463. doi: 10.1016/j.syapm.07003

PubMed Abstract | CrossRef Full Text | Google Scholar

Ismail, A., Ktari, L., Ahmed, M., Bolhuis, H., Boudabbous, A., Stal, L. J., et al. (2016). Antimicrobial activities of bacteria associated with the brown alga Padina pavonica. Front. Microbiol. 7, 1072. doi: 10.3389/fmicb.2016.01072

PubMed Abstract | CrossRef Full Text | Google Scholar

Ismail, A., Ktari, L., Ahmed, M., Bolhuis, H., Bouhaouala-Zahar, B., Stal, L. J., et al. (2018). Heterotrophic bacteria associated with the green alga Ulva rigida: identification and antimicrobial potential. J. Appl. Phycol. 30, 2883–2899. doi: 10.1007/s10811-018-1454-x

CrossRef Full Text | Google Scholar

Jiang, Z. D., Jensen, P. R., and Fenical, W. (1999). Lobophorins A and B, new antiinflammatory macrolides produced by a tropical marine bacterium. Bioorg. Med. Chem. 9, 2003–2006. doi: 10.1016/S0960-894X(99)00337-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Kang, I., Lim, Y., and Cho, J. C. (2018). Complete genome sequence of Granulosicoccus antarcticus type strain IMCC3135T, a marine gammaproteobacterium with a putative dimethylsulfoniopropionate demethylase gene. Mar. Genom. 37, 176–181. doi: 10.1016/j.margen.11005

PubMed Abstract | CrossRef Full Text | Google Scholar

Lage, O. M., and Bondoso, J. (2014). Planctomycetes and macroalgae, a striking association. Front. Microbiol. 5, 267. doi: 10.3389/fmicb.2014.00267

PubMed Abstract | CrossRef Full Text | Google Scholar

Lage, O. M., Calisto, R., Vitorino, I., and Øvreås, L. (2015). Pirellulaceae. Bergey's Manu. Sys. Archaea Bact. 3, 1–7. doi: 10.1002./9781118960608.fbm00402

CrossRef Full Text | Google Scholar

Lobban, C. S., and Wynne, M.J. eds. (1981). The Biology of Seaweeds. Univ of California Press (17).

Google Scholar

Nakanishi, K., Nishijima, M., Nomoto, A. M., Yamazaki, A., and Saga, N. (1999). Requisite morphologic interaction for attachment between Ulva pertusa (Chlorophyta) and symbiotic bacteria. Mar. Biotechnol. 1, 107–111. doi: 10.1007/PL00011744

PubMed Abstract | CrossRef Full Text | Google Scholar

Neori, A., Guttman, L., Israel, A., and Shpigel, M. (2019). Israeli-developed models of marine integrated multi trophic aquaculture (IMTA). J Coast. Res. 86(SI), 11–20. doi: 10.2112./SI86-003.1

CrossRef Full Text | Google Scholar

Nguyen, D., Masasa, M., Ovadia, O., and Guttman, L. (2023). Ecological insights into the resilience of marine plastisphere throughout a storm disturbance. Sci. Total Environ. 858, 159775. doi: 10.1016/j.scitotenv.2022.159775

PubMed Abstract | CrossRef Full Text | Google Scholar

Oksanen, J., Simpson, G., Blanchet, F., Kindt, R., Legendre, P., Minchin, P., et al. (2022). Vegan: Community Ecology Package (R Package Version 2, 6–2.). Available online at: (accessed August 28, 2023).

Google Scholar

Penesyan, A., Marshall-Jones, Z., Holmstrom, C., Kjelleberg, S., and Egan, S. (2009). Antimicrobial activity observed among cultured marine epiphytic bacteria reflects their potential as a source of new drugs. FEMS Microbiol. Ecol. 69, 113–124.

PubMed Abstract | Google Scholar

Penhale, P. A., and Capone, D. G. (1981). Primary productivity and nitrogen fixation in two macroalgae-cyanobacteria associations. Bull. Mar. Sci. 31, 64–169. doi: 10.1111/j.1574-6941.2009.00688.x

CrossRef Full Text | Google Scholar

Provasoli, L., and Pintner, I. J. (1980). Bacteria induced polymorphism in an axenic laboratory strain of Ulva lactuca (Chlorophyceae) 1. J. Phycol. 16, 196–201. doi: 10.1111/j.1529-8817.1980.tb03019.x

CrossRef Full Text | Google Scholar

Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., et al. (2012). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 41, D590–D596. doi: 10.1093/nar/gks1219

PubMed Abstract | CrossRef Full Text | Google Scholar

R Development Core Team (2010). “R: A Language and Environment for Statistical Computing.” Available online at: (accessed August 28, 2023).

Google Scholar

Ren, C. G., Liu, Z. Y., Wang, X. L., and Qin, S. (2022). The seaweed holobiont: from microecology to biotechnological applications. Microb. Biotechnol. 15, 738–754. doi: 10.1111/1751-7915.14014

PubMed Abstract | CrossRef Full Text | Google Scholar

Rosenberg, E., Koren, O., Reshef, L., Efrony, R., and Zilber-Rosenberg, I. (2007). The role of microorganisms in coral health, disease and evolution. Nat. Rev. Microbiol. 5, 355–362. doi: 10.1038/nrmicro1635

PubMed Abstract | CrossRef Full Text | Google Scholar

Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W. S., et al. (2011). Metagenomic biomarker discovery and explanation. Gen. Biol. 12, 1–18. doi: 10.1186/gb-2011-12-6-r60

PubMed Abstract | CrossRef Full Text | Google Scholar

Shahar, B., and Guttman, L. (2020). An integrated, two-step biofiltration system with Ulva fasciata for sequenced removal of ammonia and nitrate in mariculture effluents. Algal Res. 52, 102120. doi: 10.1016/j.algal.2020.102120

CrossRef Full Text | Google Scholar

Shpigel, M., Guttman, L., Ben-Ezra, D., Yu, J., and Chen, S. (2019). Is Ulva sp. able to be an efficient biofilter for mariculture effluents? J. Appl. Phycol. 31, 2449–2459. doi: 10.1007/s10811-019-1748-7

CrossRef Full Text | Google Scholar

Singh, R. P., Mantri, V. A., Reddy, C. R. K., and Jha, B. (2011). Isolation of seaweed-associated bacteria and their morphogenesis-inducing capability in axenic cultures of the green alga Ulva fasciata. Aquat. Biol. 12, 13–21. doi: 10.3354/ab00312

CrossRef Full Text | Google Scholar

Sorokin, D. Y., and Merkel, A. Y. (2023). Thiohalorhabdales ord. nov. Bergey's Man. Sys. Arch. Bact. 4, 1–3. doi: 10.1002./9781118960608.fbm00393

CrossRef Full Text | Google Scholar

van der Loos, L. M., Eriksson, B. K., and Salles, J. F. (2019). The macroalgal holobiont in a changing sea. Trends Microbiol. 27, 635–650. doi: 10.1016/j.tim.03002

PubMed Abstract | CrossRef Full Text | Google Scholar

Walters, W., Hyde, E. R., Berg-Lyons, D., Ackermann, G., Humphrey, G., Parada, A., et al. (2016). Improved bacterial 16S rRNA gene (V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys. Msystems 1, e00009–15. doi: 10.1128/msystems.00009-15

PubMed Abstract | CrossRef Full Text | Google Scholar

Wichard, T. (2022). From model organism to application: bacteria-induced growth and development of the green seaweed Ulva and the potential of microbe leveraging in algal aquaculture. Semin. Cell. Dev. Biol. 134, 69–78. doi: 10.1016/j.semcdb.04007

PubMed Abstract | CrossRef Full Text | Google Scholar

Wiegand, S., Rast, P., Kallscheuer, N., Jogler, M., Heuer, A., Boedeker, C., et al. (2021). Analysis of bacterial communities on north sea macroalgae and characterization of the isolated planctomycetes adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov. Microorganisms 9, 1494. doi: 10.3390/microorganisms9071494

CrossRef Full Text | Google Scholar

Wiese, J., Thiel, V., Nagel, K., Staufenberger, T., and Imhoff, J. F. (2009). Diversity of antibiotic-active bacteria associated with the brown alga Laminaria saccharina from the Baltic Sea. Mar. Biotechnol. 11, 287–300. doi: 10.1007/s10126-008-9143-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, J., Kobert, K., Flouri, T., and Stamatakis, A. (2014). PEAR: a fast and accurate Illumina Paired-End reAd mergeR. Bioinformatics 30, 614–620. doi: 10.1093/bioinformatics/btt593

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: Ulva fasciata, microbial assembly, temporal dynamics, succession, IMTA

Citation: Nguyen D, Ovadia O and Guttman L (2023) Temporal force governs the microbial assembly associated with Ulva fasciata (Chlorophyta) from an integrated multi-trophic aquaculture system. Front. Microbiol. 14:1223204. doi: 10.3389/fmicb.2023.1223204

Received: 17 May 2023; Accepted: 14 September 2023;
Published: 05 October 2023.

Edited by:

Neha Arora, University of South Florida, United States

Reviewed by:

Shweta Tripathi, Indian Institute of Technology Roorkee, India
Angélique Gobet, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), France

Copyright © 2023 Nguyen, Ovadia and Guttman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Lior Guttman,

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