ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1515483
Prospection forof potential new non-ribosomal peptide NRPs gene clusters infrom Bacillus genus isolated from fermented foods and soil through genome mining
Provisionally accepted
- 1Biochemistry and Applied Immunology Laboratory , Joseph KI-ZERBO University, Ouagadougou, Kadiogo, Burkina Faso
- 2School of Biomedicine and Health, Huazhong Agricultural University, Wuhan, Hubei Province, China
- 3College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China., Zhejiang, China
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Experimental studies, though often very costly, lead to the discovery of known antimicrobial products. Yet, pathogenic microorganisms are proving increasingly resistant to pre-existing antimicrobial molecules, and this is a cause for worldwide concern. Therefore, it is necessary to search for new molecules that could serve as alternatives in the food, medical and agricultural sectors. Thus, 123 complete genomes of Bacillus strains isolated from soil and fermented foods were analyzed and annotated using bioinformatics prediction and characterization tools. The view was to discover new gene clusters for the biosynthesis of non-ribosomal peptides (lipopeptides, siderophores, antibiotics). This study revealed that 83% of the genomes analyzed possess biosynthetic gene clusters for the production of siderophore bacillibactin, 61% for surfactins, 37% for fengycins, 23% for iturins, 15% for kurstakins and 3% for bacitracin. Besides, seven new biosynthetic gene clusters coding Non Ribosomal Peptide Synthetases (NRPS) have been identified in B. velezensis ATR2, B. velezensis DSYZ, B. velezensis CGMCC11640, B. amyloliquefaciens HM618, B. amyloliquefaciens WF02, B. cereus CMCC P0011, B. cereus CMCC P0021, B. subtilis SJ-10 and B. anthracis CMF9. The results of this study revealed a significant potential of the genus Bacillus to produce new non-ribosomally synthesized peptides. Now, these predicted new antimicrobial molecules can be easily studied experimentally as many new gene clusters have been identified.
Keywords: Bacillus, nonribosomal peptide, lipopeptide, ; biosynthetic gene clusters, genome mining
Received: 23 Oct 2024; Accepted: 21 May 2025.
Copyright: © 2025 Waongo, Ndayishimiye, Tapsoba, Zongo, Li and Aly. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Blaise Waongo, Biochemistry and Applied Immunology Laboratory , Joseph KI-ZERBO University, Ouagadougou, 7021, Kadiogo, Burkina Faso
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