ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1535187
This article is part of the Research TopicInsights in Microbe and Virus Interactions with Plants: 2023View all 14 articles
VdAHA1 positively regulate pathogenicity in Verticillium dahliae
Provisionally accepted
- Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China
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AHA1 (activator of HSP90 ATPase) is a co-chaperone protein that mainly performs its function by interacting with the HSP90. The biological function of AHA1has been widely reported in many species. In this study, we knocked out the VdAHA1 gene of V. dahliae by homologous recombination method. The VdAHA1 knockout mutants showed increased drug sensitivity to ergosterol synthesis pathway, significantly inhibiting ergosterol biosynthesis. The VdAHA1 knockout mutant strain also showed decreased melanin in microsclerotia by reduced expression of microsclerotia and melaninrelated genes Vaflm, Vayg1, and VdSCD. The VdAHA1 mutant showed decreased conidial production that were slightly damaged and showed moresensitivity to abiotic stresses such as temperature, SDS, CR, Sorbitol (SBT), NaCl, and KCl and decreased ATP contents. More importantly, the mutant was significantly less virulent to cotton than the wild type. This study identified the important functions of VdAHA1 in the growth, stress resistance, and virulence.
Keywords: Aha1, Verticillium dahliae, microsclerotia, stress reponse, pathogenicity
Received: 27 Nov 2024; Accepted: 22 Apr 2025.
Copyright: © 2025 Zhang, Feng, Li, Yuan, Zhou, Feng, Wei, Zhu and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yalin Zhang, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China
Hongjie Feng, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China
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