ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1553892
Identification of a novel mild isolate of Areca palm necrotic spindle-spot virus (ANSSVm) lacking two cysteine proteases (HC-Pro1 and HC-Pro2)
Provisionally accepted
- Hainan University, Haikou, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Areca palm necrotic spindle-spot virus (ANSSV) encodes two tandem cysteine proteases, HC-Pro1 and HC-Pro2, at its N-terminal region, essential for maintaining viral infectivity. Deletion of either protease from an infectious clone abolishes infectivity. We report a mild ANSSV isolate, designated ANSSVm, from areca palm leaves exhibiting mild chlorosis. ANSSVm has a truncated genome of 7,868 nt, lacking both HC-Pro1 and HC-Pro2. An infectious ANSSVm clone established systemic infection in Nicotiana benthamiana through agroinfiltration but caused no symptoms in inoculated plants. Nucleotide polymorphisms in ANSSVm clones influenced infectivity, and residue variations of coat protein (CP) and P3 were identified as critical for intercellular movement. However, amino acid substitutions in ANSSVm CP did not alter its interactions with host proteins associated with viral movement. Further research is needed to uncover host factors and interactions essential for the systemic spread of potyvirids, presenting opportunities for targeted viral control strategies in plants.
Keywords: Potyviridae, Arepavirus, Areca palm necrotic spindle-spot virus (ANSSV), Mild isolate, Infectious clone, Viral movement
Received: 31 Dec 2024; Accepted: 20 Jun 2025.
Copyright: © 2025 Tan, Cao, Wang, Zhao and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ruibai Zhao, Hainan University, Haikou, China
Xi Huang, Hainan University, Haikou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.