ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1573614
This article is part of the Research TopicInvestigating the Role of Pollutants in the Spread of Antimicrobial ResistanceView all articles
Prevalence and characterization of ESBL-producing diarrheagenic Escherichia coli originating from poultry industry in Shanghai,China
Provisionally accepted
- 1Shanghai Jinshan Disease Prevention and Control Center, Shanghai, China
- 2Microbiological laboratory, Shanghai Jinshan Disease Prevention and Control Center, Shanghai, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Antibiotic resistance genes (ARGs) in bacteria are highly prevalent in the poultry industry. These genes can transfer not only within the same species but also across different species, posing significant threats to both the poultry industry and human health. However, limited research has been conducted on the prevalence and transmission links between extended-spectrum beta-lactamase (ESBL)-producing diarrheagenic Escherichia coli (E. coli) in chickens and their living environments within Shanghai's poultry industry. In this study, 600 samples were taken from the cloacal swabs of healthy chickens and the environment of poultry farms, including feces, troughs, water boxes, and soils. Antimicrobial susceptibility testing and whole genome sequencing (WGS) were used to characterize ESBL-producing diarrheagenic E. coli. The results revealed a detection rate of E. coli at 15.67%, with the isolates exhibiting high resistance to the tested antibiotics, particularly AMP、NAL、CHL and TET. Notably, 95.45% of the isolates were multidrug-resistant (MDR). Among these, 20.45% were ESBL-producing E. coli, which exhibited higher resistance to first-through fourth-generation cephalosporins, such as CFZ、CXM、CTX. For ESBL-producing E. coli, ARGs were heavily carried, with blaTEM-1B (55.56%) and blaCTX-M-55 (38.89%), being popular in the farm. The dominant sequence type (ST) was ST1286, the prevalent serotype was O16:H32, and the dominant CH type was FumC174/FimH23. STs with the same serotypes and FumC-FimH (CH) types showed high genetic relatedness in SNP-based phylogenetic analysis, highlighting the need for enhanced surveillance to prevent further transmissions.
Keywords: ARGS, ESBL-producing diarrheagenic E. coli, WGS, MDR, SNP
Received: 09 Feb 2025; Accepted: 29 May 2025.
Copyright: © 2025 Que, Yuan, Xu, Liu, Yang and Long. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ye Yu Long, Microbiological laboratory, Shanghai Jinshan Disease Prevention and Control Center, Shanghai, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.