ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Phage Biology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1585026
Characterization and genomic analysis of two Aeromonas phages
Provisionally accepted
Li ling Jiang1Chang Liu2Guang feng Liu2Xiu hua Tao3Hong sai Zhang2Yue Zhang2Yuyu Chen2Meng yao Li2
Jing zhe Jiang2*
Hong peng Chen1*- 1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China
- 2Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China
- 3Institute of Forest Medicinal Materials and Food, Jiangxi Academy of Forestry, Nanchang, Jiangxi Province, China
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Phages, as viruses that specifically infect certain bacteria, have great research value in the prevention and control of bacterial diseases. Bacteria of the genus Aeromonas are widely distributed in aquatic environments and are important zoonotic pathogens that can cause a variety of diseases. In this study, two virulent phages, AhC3_1 and AsC4_1, were successfully isolated using bacteria from the genus Aeromonas. The results showed that the genome sequences of phages C3 and C4 were 232,884 bp and 45,983 bp in length, respectively, with G+C contents of 44.36% and 50.11%. Both belong to double-stranded circular DNA viruses. Phages C3 and C4 have been classified within two novel viral genera, provisionally designated as "Aynavirus" and "Asnavirus" respectively. Additionally, both C3 and C4 exhibited good stability in different temperature and pH conditions. The latent period of C3 was 10 minutes, with a burst size of 32 PFU.cell⁻¹. The in vitro antibacterial curve showed that when the multiplicity of infection (MOI) was 10⁻³, C3 could exert a strong inhibitory effect on Aeromonas hydrophila for up to 10 hours. The latent period of C4 was less than 10 minutes, with a burst size of 1250 PFU.cell⁻¹. At an MOI of 10⁻¹, C4 could exert a strong inhibitory effect on Aeromonas salmonicida for up to 15 hours. In summary, the two phages isolated in this study have the potential to serve as potential therapeutic agents for aquaculture diseases and provide valuable data for research on antibacterial infections.
Keywords: Aeromonas phages, Biological characteristics, Genomic Analysis, phylogenetic tree, Antibacterial curve
Received: 28 Feb 2025; Accepted: 12 May 2025.
Copyright: © 2025 Jiang, Liu, Liu, Tao, Zhang, Zhang, Chen, Li, Jiang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jing zhe Jiang, Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China
Hong peng Chen, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, China
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