ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1595418
One mechanism of spleen-kidney yang deficiency IBS-D: intestinal microbiota affect ATPase
Provisionally accepted
- Hunan University of Chinese Medicine, Changsha, China
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Background: This study aimed to investigate the effects of adenine and Folium senna combined with restraint tail-clamping stress method on the intestinal microbiota of mice with spleen-kidney yang deficiency type diarrhea predominant irritable bowel syndrome (IBS-D) from the perspective of energy metabolism. Methods: Twenty SPF-grade female mice were randomly divided into two groups, normal group (CN group) and model group (MD group), with 10 mice per group. A spleenkidney yang deficiency IBS-D model was established using adenine and Folium senna combined with restraint tail-clamping stress. After model establishment, enzyme-linked immunosorbent assay (ELISA) was used to detect the activity of Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase; spectrophotometry was used to measure intestinal digestive enzyme activity; fluorometric diacetate (FDA) hydrolysis spectrophotometry was used to detect intestinal microbial activity; and 16S rRNA sequencing was performed to identify intestinal microbiota in the intestinal contents. Results: (1) ELISA showed a significant decrease in Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase activity in spleenkidney yang deficiency IBS-D model mice. (2) Intestinal enzyme activity analysis revealed increased lactase and amylase activity, and decreased sucrase activity in model mice. (3) Intestinal microbial activity analysis demonstrated significantly enhanced in microbial activity in spleenkidney yang deficiency IBS-D model mice. (4) Correlation analysis revealed a significant positive correlation between Heminiphilus, Clostridium, Desulfovibrio and Ca 2+ -Mg 2+ -ATPase, a significant positive correlation between Heminiphilus and Na + -K + -ATPase, a significant positive correlation between Pediococcus and sucrase, and a significant negative correlation between Clostridium and sucrase. Conclusion: Reduced Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activities, increased sucrase and amylase activities, decreased lactase activity, and enhanced intestinal microbial activity may constitute important mechanisms underlying the development of spleen-kidney yang deficiency IBS-D.
Keywords: intestinal microbiota, ATPase, spleen-kidney yang deficiency IBS-D, Energy Metabolism, Intestinal digestive enzymes
Received: 19 Mar 2025; Accepted: 22 Jul 2025.
Copyright: © 2025 Long, Li, Deng and Tan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhoujin Tan, Hunan University of Chinese Medicine, Changsha, China
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