ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Aquatic Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1595680
This article is part of the Research TopicProbiotics in Aquaculture: Enhancing Health and SustainabilityView all articles
Effects of Bacillus licheniformis on the water quality, growth performance and bacterial community in Penaeus vannamei aquaculture system
Provisionally accepted
- Fujian Normal University, Fuzhou, China
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This study aimed to investigate the impact of Bacillus licheniformis on the Penaeus vannamei aquaculture system. The design of the aquaculture experiment comprised two groups: a control group (CK) fed a basal diet and a treatment group (PB) was fed the same diet with addition of B. licheniformis (5 × 10 4 CFU/mL) into the water every five days. These groups were systematically evaluated through comprehensive water quality analyses, growth performance assessments, and high-throughput 16S rRNA gene sequencing. The results demonstrated that B. licheniformis significantly improved water quality, promoted shrimp growth, and altered the bacterial community structure: (1) B. licheniformis significantly reduced the concentrations of ammonia nitrogen, nitrite nitrogen, and pathogenic Vibrio counts in the later stages of cultivation (P <0.05), while significantly promoting shrimp growth; (2) The addition of B. licheniformis increased the diversity and richness of bacteria both in the water and shrimp intestinal tracts, leading to significant changes in bacterial community structure. It also enhanced beneficial bacterial genera such as Gemmobacter, Paracoccus, and Bacillus in the water, while concurrently reducing the potential pathogenic Flavobacterium in the shrimp intestinal tract; (3) The dominant bacterial populations were significantly affected in both water and shrimp intestinal samples. In water, Aurantimicrobium was the predominant genus in both groups, with the PB group showing a notably lower relative abundance. In the shrimp intestines, the CK group was dominated by Gemmobacter and Fluviicola, while Aurantimicrobium prevailed in the PB group. In conclusion, the study revealed the potential of B. licheniformis in shrimp aquaculture by improving water quality, promoting shrimp growth, and modulating bacterial community structure.
Keywords: Penaeus vannamei aquaculture, Bacillus licheniformis, high-throughput sequencing, Bacterial community analysis, Water Quality, growth performance
Received: 18 Mar 2025; Accepted: 30 Apr 2025.
Copyright: © 2025 Wu, Yi, Tang, Li, Fan and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zheng Yi, Fujian Normal University, Fuzhou, China
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