ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1601035
This article is part of the Research TopicMetagenomic Approach for Exploration of Antimicrobial Resistance in Uncultivated MicrobiotaView all 6 articles
Identification of an IncHI5-like plasmid co-harboring bla NDM-1 and bla OXA-1 in mcr-8.1-positive Klebsiella pneumoniae isolate
Provisionally accepted
- Zhengzhou University, Zhengzhou, China
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The emergence of polymyxin-resistant carbapenem-resistant Klebsiella pneumoniae (CRKP) severely limits clinical treatment options and poses a significant threat to anti-infective therapy. In this study, we investigate the genetic characteristics of the IncHI5-like plasmid co-harboring blaNDM-1 and blaOXA-1 in an mcr-8.1-positive clinical CRKP isolate. The ST626 CRKP strain KP19-2581, isolated from a human clinical infection, exhibited a multidrug-resistant (MDR) phenotype. Whole-genome sequencing revealed that the colistin resistance gene mcr-8.1 was located on an IncFIA/IncFII plasmid, flanked by the conserved structure IS903B-orf-mcr-8.1-orf-IS903B. In addition, strain KP19-2581 carried a novel IncHI5-like MDR plasmid, designated pKP19-2581-367k-HI5-NDM1, which co-harbored the blaNDM-1 and blaOXA-1 genes. This plasmid contained two MDR regions, harboring a diverse array of resistance genes across multiple antibiotic classes. The dissemination of blaOXA-1 in variable region is related to the structure of class 1 integron, while IS26 mediates the integration of blaNDM-1 on IncHI5-like plasmid. Notably, this is the first report of an IncHI5-like plasmid carrying both blaNDM-1 and two copies of blaOXA-1, along with multiple resistance genes and insertion sequences. Given its potential to acquire additional resistance determinants, this plasmid may serve as a reservoir for further antimicrobial resistance evolution, underscoring the urgent need for surveillance of IncHI5 plasmids to mitigate their clinical and epidemiological impact.
Keywords: Klebsiella pneumoniae, Carbapenem-resistant, mcr-8.1, IncHI5-like, Genomics
Received: 27 Mar 2025; Accepted: 22 Apr 2025.
Copyright: © 2025 Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yan Li, Zhengzhou University, Zhengzhou, China
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