ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Food Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1606352
Heat-killed Bacillus subtilis Concerning Broilers' Performance, Cecal Architecture and Microbiota
Provisionally accepted
- Jinzhou Medical University, Jinzhou, Liaoning Province, China
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At present, Bacillus subtilis has been reported as a probiotic to be used as a feed additive to improve the production performance of broilers. However, the relevant reports on its derived postbiotics are still limited.In this study, a total of 480 day-old Arbor acre broiler chicks with an initial body weight of 52.8 ± 1.4 g were used in this study to investigate the effects of dietary supplementation of graded levels of heat-killed B. subtilis on growth performance, cecal morphology, and cecal bacteria and fungus composition. Birds were assigned into four groups with six replicates of 20 birds eaxh. The experiment was designed to last for 42 days. B. subtilis was supplemented with 0.000, 0.015, 0.030, or 0.045% heat-killed Bacillus subtilis (HKB). Results indicated that body weight gain, gain to feed ratio, cecal villus height, and cecal villus to crypt ratio dosedependently improved, while cecal crypt depth dose-dependently reduced, by HKB supplementation.Additionally, dietary supplementation of graded levels of HKB dose-dependently reduced cecal bacteria Chao1 index. Cecal bacteria identified by 16S rRNA and cecal fungus identified by ITS revealed that the supplementation of HKB regulate the composition of bacteria and fungus in cecum. Therefore, dietary supplementation of HKB is an effective measure to improve cecal morphology by regulating cecal bacteria and fungus composition, and therefore improved growth performance.
Keywords: additive, Hindgut, Intestinal health, intestinal fungus composition, ITS, Postbiotic
Received: 05 Apr 2025; Accepted: 28 May 2025.
Copyright: © 2025 Cui, Meng, He, Chen, Liu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Desheng Li, Jinzhou Medical University, Jinzhou, 121001, Liaoning Province, China
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