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ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Food Microbiology

Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1606628

Altering the bacterial community: Sour soup decreased CO 2 production and improved the fermentation quality of Broussonetia papyrifera, Tritriale and mixed silages

Provisionally accepted
Qiming  ChengQiming Cheng1*Dianpeng  LiuDianpeng Liu2Lei  YaoLei Yao1Maoya  LiMaoya Li1Yulian  ChenYulian Chen1Jiachuhan  WangJiachuhan Wang1Xiangjiang  HeXiangjiang He1Yuanyuan  ZhaoYuanyuan Zhao1Chao  ChenChao Chen1*Xiaoqing  ZhangXiaoqing Zhang3
  • 1Guizhou University, Guiyang, China
  • 2Tongliao institude of agriculture and animal husbandry, Tongliao, Inner Mongolia Autonomous Region, China
  • 3Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia Autonomous Region, China

The final, formatted version of the article will be published soon.

Carbon dioxide (CO2) generated during the ensiling process is a source of greenhouse gas emissions and a reason for the loss of nutrients during ensiling.Broussonetia papyrifera (B), Tritriale (T) and their mixtures (B7T3, B5T5 and B3T7) were ensiled with sour soup (S) and Lactobacillus acidophilus (LAB) to investigate the effects of additives on silage quality, CO2 production and bacterial communities.After 45 days of fermentation, the B3T7 treatment resulted in the lowest CO2 production, a relatively high lactic acid content (pH<4.2), and relatively high relative abundances of Lactiplantibacillus and Weissella after fermentation; the quality of silage in all the treatments with additives was greater than that in the CK treatment, and the CO2 content was significantly lower than that in the CK treatment (P<0.05).In addition, the overall silage quality was better than that of CK after the addition of additives, the CO2 content was significantly lower (P<0.05), and adding sour soup resulted in a greater effect than adding LAB. CO2 production was positively correlated with the relative abundances of Lactococcus, Enterococcus, and Neoscardovia and negatively correlated with the relative abundances of Lactiplantibacillus, Weissella, and Sphingomonas. In summary, selecting an appropriate proportion of different forages for mixed silage and adding sour soup may be effective ways to improve silage quality and reduce CO2 production during ensiling.

Keywords: Sour soup, Silage, Carbon Dioxide, Bacterial communities, Fermentation

Received: 07 Apr 2025; Accepted: 03 Jun 2025.

Copyright: © 2025 Cheng, Liu, Yao, Li, Chen, Wang, He, Zhao, Chen and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Qiming Cheng, Guizhou University, Guiyang, China
Chao Chen, Guizhou University, Guiyang, China

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