ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Food Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1607824
Lactobacillus plantarum SAL delays aging-associated oxidative stress and gut microbiota dysbiosis in mice
Provisionally accepted
- 1Putian University, Putian, China
- 2Key Laboratory of Screening and Control of Infectious Diseases, Fujian Provincial University, Quanzhou Medical College, Quanzhou, quanzhou, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
This study investigated Lactobacillus plantarum SAL's ability to reduce oxidative stress and regulate gut microbiota in D-galactose-induced aging mice, suggesting its potential anti-aging effects. 24 SPF KM male mice were divided into a control group (CON), model group (MOD), and a SAL strain intervention group (SAL).MOD and SAL groups received D-gal-induced aging models. SAL group was orally administered SAL strain suspension daily, while MOD and CON groups received saline for 10 weeks. After the intervention, serum and liver tissues were collected to detect aging biomarkers (β-galactosidase) and oxidative stress markers. Colon tissue histopathological examination was performed, and fresh fecal samples were subjected to metagenomic sequencing and analysis. Additionally, Spearman correlation analysis was conducted to evaluate the relationships between genus-level differential gut microbiota and oxidative stress markers in serum and liver tissues. Compared with the MOD group, the SAL group exhibited significantly reduced MDA levels in serum and liver tissues (all P < 0.05), elevated activities of SOD and T-AOC (all P < 0.05), and increased serum GSH-Px and CAT activities (all P < 0.05). Colon histology showed structural improvements, including increased crypt numbers, restored architecture, reduced submucosal space, and upregulated expression of ZO-1, Occludin, and Muc2 (all P < 0.05). Gut microbiota analysis revealed increased abundances of Firmicutes and Verrucomicrobia, decreased Bacteroidetes, and elevated Firmicutes/ Bacteroidetes (F/B) ratio (P < 0.05). Differential genera Lactobacillus and Mucispirillum showed significant negative correlations with MDA levels (all P < 0.05), while Lactobacillus positively correlated with SOD, GSH-Px, and T-AOC activities.The SAL strain intervention significantly improved redox homeostasis, restored intestinal barrier integrity, and reversed gut dysbiosis, highlighting its dual regulatory role in anti-aging mechanisms. These findings demonstrate the potential of Lactobacillus plantarum SAL as an anti -aging probiotic and establish a theoretical framework for microbiota -targeted interventions to alleviate age-related pathologies.
Keywords: Aging, SAL strain, Lactobacillus plantarum, Oxidative Stress, gut
Received: 08 Apr 2025; Accepted: 28 May 2025.
Copyright: © 2025 DONG, lun, sun and LIU. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: WEN DONG, Putian University, Putian, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.