ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1608076
This article is part of the Research TopicNew Horizons in Gut Microbiome Research for Enhancing Livestock ProductivityView all articles
Effect of dietary supplementation with Brevibacillus laterosporus on broiler growth performance, meat quality and gut microbiome
Provisionally accepted
- Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, China
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This study explored the impact of Brevibacillus laterosporus on broilers. 320 one-day-old YS909 male broiler chicks was randomly assigned to four groups, each comprising eight replicates with 10 chicks. The groups were as follows: 1) a control group receiving a basal diet (CON); 2) a low-dose group receiving a basal diet supplemented with 100 mg/kg of B. laterosporus (LBL); 3) a medium-dose group receiving a basal diet supplemented with 300 mg/kg of B. laterosporus (MBL); and 4) a high-dose group receiving a basal diet supplemented with 500 mg/kg of B. laterosporus (HBL). Dietary supplementation with B. laterosporus significantly decreased the feed intake / weight gain(F/G) in LBL (22-42 d and 1-42 d), MBL (22-42 d) and HBL (22-42 d) groups. Both MBL and HBL groups showed higher semieviscerated percentages than the control. The MBL group had a significantly increased eviscerated percentage. The LBL group had a significantly increased breast muscle percentage. Specifically, the HBL group exhibited a notable increase in muscle C18:3n3 content, and a significant decrease in muscle C18:1n9t and C20:3n3 content.The LBL group saw significant reductions in the proportion of C18:1n9t and C20:1.Additionally, the MBL group experienced significant decreases in the proportions of C18:3n3, C20:3n3 and C20:4n6. Dietary supplementation with B. laterosporus significantly enhanced the oxidative stress resistance of serum by decreasing malondialdehyde (MDA) levels and increasing glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC). 16S ribosomal DNA and metabolome sequencing of cecum contents was conducted for the MBL and CON groups. This analysis demonstrated significant increases in α-diversity indices in the MBL group. There was an increased relative abundance of Firmicutes and a decreased relative abundance of Bacteroidetes and Proteobacteria in the MBL group. In addition, the shifts of the cecal microbial community lead to the alteration of metabolites of the cecum including amino acid and lipid. In conclusion, dietary supplementation with medium-dose B. laterosporus enhanced broiler carcass traits and antioxidant status by modulating cecal microbiota and metabolites, demonstrating its potential as an effective feed additive.
Keywords: B. laterosporus, broilers, growth performance, meat quality, antioxidant capacity, Gut Microbiota
Received: 08 Apr 2025; Accepted: 21 May 2025.
Copyright: © 2025 Han, Fu, Wang, Sun, Cao, Lei, Zhou, Li, Liu, Li, Hao and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jie Liu, Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan, China
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