ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Symbioses
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1608297
This article is part of the Research TopicMicrobiota, Antibiotic Resistance, and Host-Microbe Interactions: A Comprehensive Exploration of Infectious Disease DynamicsView all 5 articles
The differences in microbial composition may be an important cause of pancreatic pseudocyst infection - an observational study based on 16SrRNAMicrobial composition differences lead to pancreatic pseudocyst infection - an observational study based on 16SrRNA
Provisionally accepted
- Second Hospital of Hebei Medical University, Shijiazhuang, China
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Abstract Background: There are no studies of pancreatic pseudocyst infections related to microorganisms. The purpose of this study was to analyze the microbiological differences between infective and non-infective pseudocysts. Methods: This was an observational cohort study. Thirty-seven patients with pancreatic pseudocyst who underwent EUS drainage at our center were included in the study. According to postoperative infection, the patients were divided into infected group and non-infected group. Capsular fluid was collected during endoscopic drainage and microbial sequencing was performed. Results: The clinical features of the two groups were similar (P > 0.05). There was no significant difference in α diversity between infected and non-infected groups (P > 0.05). There was significant difference in β diversity between infected and uninfected groups (Adonis, R2 = 0.039, p = 0.019). Random forest maps identified the top five species with the greatest abundance differences. At the genus level, the relative abundance of Klebsiella, Streptococcus, Collinsella, Phascolarctobacterium and Megamonas Klebsiella, streptococcus, Escherichia coli, Phascolarctobacterium and macroomonas in the sac fluid of infected group was significantly higher than that of non-infected group. Conclusion: The differences in the microbial composition of the cyst fluid in pancreatic pseudocyst may have an impact on postoperative infections. The relative abundance of Klebsiella, Streptococcus, Collinsella, Phascolarctobacterium and Megamonas in infected group was significantly higher than that in noninfected group. Further research is still needed in the future to confirm this.The difference of microbial composition in cystic fluid of pancreatic pseudocyst affects the infection. The relative abundance of Klebsiella, streptococcus, Escherichia coli, Phascolarctobacterium and macroomonas in infected group was significantly higher than that in non-infected group.
Keywords: Microorganism, Pancreatic Pseudocyst, Infection, 16sRNA, EUS
Received: 16 May 2025; Accepted: 22 Oct 2025.
Copyright: © 2025 Li, Zhao, Hou and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Lichao Zhang, zhanglichao@hebmu.edu.cn
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