ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Sishen Pill Treats Spleen-Kidney Yang Deficiency IBS-D by Influencing ATPase Activity and Intestinal Microbiota
Provisionally accepted
- Hunan University of Chinese Medicine, Changsha, China
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Background: Sishen Pill (SSP) has therapeutic effects on spleen-kidney yang deficiency diarrhea-predominant irritable bowel syndrome (IBS-D). IBS-D is characterized by intestinal microbiota dysbiosis and impaired ATPase activity. SSP treatment restores microbiota balance and improves ATPase activity, suggesting it exerts therapeutic effects by modulating the microbiota and restoring ATPase function. Methods: Forty SPF-grade female mice were randomly divided into four groups: normal (CC), spontaneous recovery (MC), pinaverium bromide treatment (PBT), and SSP treatment (SSP). IBS-D was induced using adenine and Folium sennae combined with restraint-tail clamping stress. After model validation, SSP aqueous extract was administered. Evaluations included behavioral assessments, Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activity quantification via ELISA, intestinal digestive enzyme activity and microbial activity measurements, and microbiota composition analysis through 16S rRNA gene sequencing. Results: (1) SSP treatment significantly increased Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activities (p < 0.05). (2) Digestive enzyme activities, including lactase and amylase, increased significantly (p < 0.01). (3) Intestinal microbial activity was notably enhanced (p < 0.01). (4) Correlation analysis showed that Clostridioides was positively correlated with lactase (p < 0.01) and microbial activity (p < 0.05), while Desulfovibrio negatively correlated with microbial activity (p < 0.01). In comparisons between PBT and SSP groups, Ca²⁺-Mg²⁺-ATPase positively correlated with Maribacter and Scatolibacter (p < 0.05), while amylase negatively correlated with Scatolibacter (p < 0.01). Conclusion: SSP significantly alleviates symptoms of spleen-kidney yang deficiency in IBS-D by regulating intestinal microbiota, increasing ATPase activity, and enhancing digestive enzyme and microbial activity.
Keywords: Sishen Pill, intestinal microbiota, spleen-kidney yang deficiency IBS-D, ATPase activity, intestinal digestive enzyme activity
Received: 14 Apr 2025; Accepted: 28 Oct 2025.
Copyright: © 2025 Long, Li, Tan and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhoujin Tan, tanzhjin@sohu.com
Na Deng, 243671178@qq.com
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