ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1611576
This article is part of the Research TopicPathogenic microorganisms and biosafetyView all 12 articles
Comparative disinfection efficacy in aquaculture: novel methylene bis(thiocyanate) vs conventional trichloroisocyanuric acid against Aeromonas hydrophila
Provisionally accepted
Guang Yang1Ying Huang2Ning Ma2Kai Li2Xiao-Mei Wang2Lian-Bo Zhang1Wen-Bo Yang2Wan-Li Zhang2Lei Xia2
Hongyu Zhang2*Li-Lai Yuan2- 1Fisheries Engineering Institute, Chinese Academy of Fishery Sciences, Beijing, China
- 2Chinese Academy of Fishery Sciences (CAFS), Beijing, Beijing Municipality, China
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Aeromonas hydrophila is a major pathogen responsible for significant economic losses in global aquaculture. Inhibitory and bactericidal activities of seven disinfectants were tested against ten aquatic pathogens, including A. hydrophila, A. veronii, A. salmonicida, A. sobria, Edwardsiella tarda, E. ictaluri, Pseudomonas aeruginosa, and Yersinia ruckeri. Minimal effective concentrations were determined via quantitative suspension tests, while a scale-trauma zebrafish model assessed in vivo protection. Methylene bis(thiocyanate) (MBT) exhibited the strongest antimicrobial activity, with minimum inhibitory concentrations and minimum bactericidal concentrations of 0.1 to 0.4 mg/L and 0.2 to 0.8 mg/L, respectively. At 0.01 mg/L, MBT achieved a 3-log pathogen inactivation, equivalent to 0.2 mg/L Trichloroisocyanuric acid (TCCA). In vivo, 字体: 倾斜 设置格式[宏阅]: and 删除[xuehu]: MBT 删除[宏阅]: TCCA 删除[宏阅]: 字体: 倾斜 设置格式[宏阅]:0.01 mg/L MBT provided 100% protection in zebrafish after 6 and 12 hours exposure, whereas 0.2 mg/L TCCA yielded 95.83 ± 3.61% survival after 1 hour. The safe concentrations of MBT and TCCA for zebrafish were 0.0364 and 0.0677 mg/L, respectively. The results showed that both TCCA and MBT effectively controlled A. hydrophila infection; however, MBT demonstrated greater potential for aquaculture applications due to its lower effective concentration (0.01 mg/L) and reduced sensitivity to interference from organic matter. In addition, this study presents a systematic protocol for evaluating the efficacy of disinfectants.
Keywords: Aeromonas, methylene bis (thiocyanate), trichloroisocyanuric acid, Disinfection, Aquaculture
Received: 14 Apr 2025; Accepted: 09 Jul 2025.
Copyright: © 2025 Yang, Huang, Ma, Li, Wang, Zhang, Yang, Zhang, Xia, Zhang and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hongyu Zhang, Chinese Academy of Fishery Sciences (CAFS), Beijing, 100141, Beijing Municipality, China
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