ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Symbioses
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1616937
Analysis of microorganisms and drug-resistance mutations detected by Probe-Capture Metagenomics among HIV-infected patients with pneumonia
Provisionally accepted
Qianhui Chen1
Pingzheng Mo1
Rongrong Yang1
Huan Xu2
Liu Huifang2
Zhongwei Zhang1
Qian Du1
Qunqun Jiang1Qinglian Guo3
Liang-jun Chen3
Yongxi Zhang1*
Yong Xiong1*Liping Deng1*- 1Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China
- 2Vision Medicals Co. Ltd, Guangzhou, China
- 3Department of Clinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan, China
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Background Probe-Capture Metagenomics is a newly developed method for detecting infectious pathogens. However, its application in HIV-infected patients with pulmonary infection remains limited. This study utilized Probe-Capture Metagenomics to analyze lung microbiomes and Drug Resistance Mutations of HIV and bacteria in people living with HIV (PLWH) with pneumonia. Methods We retrospectively investigated lung microorganisms in PLWH hospitalized at Zhongnan Hospital of Wuhan University. A combination of bronchoalveolar lavage fluid Probe-Capture Metagenomics and conventional microbiological tests were performed in all patients.Results A total of 91 patients were included in the study. Excluding the EB and Torque teno virus, at least two organisms were identified in 85 patients using Probe-Capture Metagenomics combined with conventional microbiological tests. The top six detected organisms were CMV, Pneumocystis jirovecii, Mycobacterium tuberculosis complex, HHV-7, Candida albicans and Aspergillus. For specific organisms, the detection rate of CMV and Candida albicans by Probe-Capture Metagenomics was significantly higher than that of conventional microbiological tests (p<0.0001).
Keywords: microorganisms, Probe-Capture Metagenomics, HIV, Pneumonia, Drug Resistance
Received: 24 Apr 2025; Accepted: 17 Jul 2025.
Copyright: © 2025 Chen, Mo, Yang, Xu, Huifang, Zhang, Du, Jiang, Guo, Chen, Zhang, Xiong and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yongxi Zhang, Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China
Yong Xiong, Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China
Liping Deng, Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, Wuhan, China
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