ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1617913
This article is part of the Research TopicPlant Virus Dynamics in Farming SystemsView all 3 articles
Development of antibody-cocktail for simultaneous detection of ASGV, ACLSV and ASPV
Provisionally accepted
- 1Amity Institute of Virology and Immunology, Amity University, Noida, Uttar Pradesh, India
- 2Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Production of apple at both quantitative and qualitative level is hampered on a large scale due to multiple viral infection causing huge economic losses. Mixed infection of apple stem grooving virus(ASGV), apple stem pitting virus(ASPV) and apple chlorotic leaf spot virus(ACLSV) are common in apple trees. Diagnosis of viruses by serological techniques requires development of antibodies against each virus individually (mostly exported in India), which is not only costly but also time-consuming. Therefore, we designed a triple construct gene for expressing a recombinant protein constituting conserved region in coat protein(CP) of ASGV, ASPV and ACLSV, for the development of an antibodycocktail against three apple viruses for their simultaneous detection in a limited time. We have tried to develop an antibody-cocktail that can perform diagnostics for three different apple viruses in a single ELISA assay, that would not only be time-saving but also cost-effective for the purpose of testing large samples during certification of plants in nurseries. To our knowledge, this is the first report of diagnosis of multiple viruses of apple in a single ELISA assay by using antibody-cocktail.
Keywords: ASGV, ACLSV, ASPV, Antibody-Cocktail, ELISA
Received: 25 Apr 2025; Accepted: 01 Aug 2025.
Copyright: © 2025 Suman, Dhir and Chandel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Vanita Chandel, Amity Institute of Virology and Immunology, Amity University, Noida, 201301, Uttar Pradesh, India
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.