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ORIGINAL RESEARCH article

Front. Microbiol.

Sec. Antimicrobials, Resistance and Chemotherapy

Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1618419

This article is part of the Research TopicAdvancing Antimicrobial Strategies: Nucleic Acid and Peptide-Based ApproachesView all 10 articles

Exploring the Antibacterial Potential of Arthrocolins Against Extensively drug-resistant Pseudomonas aeruginosa : Mechanistic Insights into Amino Acid Metabolism Disruption

Provisionally accepted
Baorui  YangBaorui Yang1Yunwen  ZhangYunwen Zhang1Wu  QunfuWu Qunfu2Jianmei  LiuJianmei Liu1Guangjuan  WuGuangjuan Wu1Weijuan  TianWeijuan Tian1Deyao  DengDeyao Deng1*Xuemei  NiuXuemei Niu2*Wenli  YuanWenli Yuan1*
  • 1The Affiliated Hospital of Yunnan University, Kunming, China
  • 2Yunnan University, Kunming, Yunnan Province, China

The final, formatted version of the article will be published soon.

Extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) poses a serious clinical threat owing to its intrinsic resistance mechanisms and the lack of effective therapeutic agents. In this study, we evaluated the antibacterial activity of arthrocolins (Acs), a novel group of xanthene-like compounds isolated from Escherichia coli, against XDR-PA. Clinical data analysis identified prolonged antimicrobial therapy, invasive procedures, and extended hospitalization as significant risk factors for XDR-PA infection. Drug susceptibility testing demonstrated that Acs markedly inhibited XDR-PA growth at low micromolar concentrations (IC50 = 3.094 μ M). Transmission electron microscopy revealed cell wall disruption and cytoplasmic condensation in Acs-treated bacteria. Integrated transcriptomic and metabolomic analyses further indicated that Acs interferes with amino acid metabolism, leading to impaired energy production and abnormal lipid accumulation. These findings demonstrate that Acs exerts potent antibacterial effects through disruption of metabolic homeostasis and structural integrity, highlighting its potential as a promising candidate for the treatment of XDR-PA infections.

Keywords: extensively drug-resistant Pseudomonasaeruginosa, Arthrocolins, theantibacterial effect, Transcriptomics, Metabolomics

Received: 26 Apr 2025; Accepted: 03 Sep 2025.

Copyright: © 2025 Yang, Zhang, Qunfu, Liu, Wu, Tian, Deng, Niu and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Deyao Deng, The Affiliated Hospital of Yunnan University, Kunming, China
Xuemei Niu, Yunnan University, Kunming, 650500, Yunnan Province, China
Wenli Yuan, The Affiliated Hospital of Yunnan University, Kunming, China

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