ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Infectious Agents and Disease
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1620907
This article is part of the Research TopicPathogenic microorganisms and biosafetyView all 6 articles
Characterization of bla NDM-5 -carrying plasmids in two clinical Salmonella isolates from Jiaxing City, China
Provisionally accepted
Ping Li1*
Yongjuan Yuan2Yong Yan1Miaomiao Jia1Lei Gao1Xuejuan Liu1Yangming Sun1Guoying Zhu1Zhongwen Chen1- 1Jiaxing Center for Disease Control and Prevention, Jiaxing, China
- 2Jiashan County Center for Disease Control and Prevention, Jiaxing, China
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The worldwide emergence of bla NDM -positive carbapenem-resistant Salmonella enterica isolates in recent years poses a huge public health challenge. In this study, we characterized two clinical S. enterica isolates carrying bla NDM-5 : a serotype 4,[5],12:i:-strain (2023JX045) and a serovar Stanley strain (2024-406). Both isolates were multidrug resistant, with insusceptibility to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefuroxime, ceftiofur, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, ceftazidime/avibactam, meropenem, imipenem, ertapenem, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, florfenicol, chloramphenicol, ciprofloxacin, colistin, and polymixin B. The bla NDM-5 -carrying plasmids in 2023JX045 and 2024-406 were named p23045-NDM5 and p2024406-NDM5, respectively, with both belonging to the incompatibility (Inc)HI2/IncHI2A group and sequence type ST3. In 2023JX045, bla NDM-5 was flanked by two same-oriented copies of IS26 elements (IS26-dsbC-trpF-ble MBL -bla NDM-5 -IS5-△IS3000-△ISKox3-umuC-umuD-IS26). In 2024-406, p2024406-NDM5 was found to carry two copies of bla NDM-5 , possibly resulting from duplication of IS26-dsbC-trpF-ble MBL -bla NDM-5 -IS5-△ISAba125-△IS3000-△ISKox3-umuC-umuD-I S26 and interrupted by mobile element IS1 at the upstream region of △IS3000. This is the first report to describe the presence of two bla NDM-5 copies on an IncHI2/IncHI2A plasmid carried by serovar Stanley, as well as the dissemination of bla NDM-5 in Salmonella in Jiaxing City, China. The dissemination of bla NDM in Salmonella isolates and the complexity of the bla NDM-5 region highlight the urgent need to monitor carbapenem-resistant S. enterica.
Keywords: Non-typhoidal Salmonella, Carbapenem-resistant, IncHI2/IncHI2A, IS26 Unit, blaNDM-5
Received: 30 Apr 2025; Accepted: 05 Jun 2025.
Copyright: © 2025 Li, Yuan, Yan, Jia, Gao, Liu, Sun, Zhu and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Ping Li, Jiaxing Center for Disease Control and Prevention, Jiaxing, China
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