ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiotechnology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1622784
This article is part of the Research TopicMicrobial Remediation of Heavy Metal PollutionView all 3 articles
Isolation of a novel manganese-oxidizing bacterium Lysinibacillus xylanilyticus M125: Characterization, structural evolution and Cdadsorption activity of biogenic Mn oxides produced by the strain
Provisionally accepted
- Shandong University (Qingdao), Qingdao, China
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Manganese-oxidizing bacteria play a critical role in converting Mn(II) to Mn(III/IV) oxides, which have been widely applied to control environmental pollution, particularly that caused by heavy metals. In this study, a novel manganese-oxidizing bacterial strain was isolated from Mncontaminated soil near an electroplating factory and identified it as Lysinibacillus xylanilyticus M125.The Mn oxidation ability of this strain was studied. Meanwhile, the influence of different pH, temperature, and Mn 2+ concentrations on bacterial growth and Mn 2+ biological oxidation were evaluated. Strain M125 grew well within the pH range of 5.0~10.0 and temperature range of 15 ℃~40 ℃. It also tolerated Mn 2+ concentration up to 60 mM, indicating strong environmental resilience and potential for practical application. The biogenic manganese oxides (BioMnOx) produced by strain M125 were comprehensively characterized using TEM, XRD, XPS and FTIR techniques. BioMnOx had a relatively complex structure, containing a mixture of amorphous MnO2 and crystalline Mn3O4 phase. Also, BioMnOx existed in various morphologies, including nanosheet, globular structures encased in sheaths, and extracellularly dispersed forms. Long-term cultivation further elucidated the morphological evolution of these oxides. Given the high surface area and porous nature of BioMnOx, its capacity for cadmium adsorption was also assessed. Over 99.5% of cadmium ions in water are adsorbed and removed by strain M125, highlighting its potential for cadmium pollution remediation. Overall, this work introduces a new bacterial resource for Mn and Cd bioremediation and offers detailed insights into the structural and functional characteristics of BioMnOx, supporting its application in environmental biotechnology.
Keywords: Lysinibacillus xylanilyticus, Manganese-oxidizing bacteria, Biogenic manganese oxides, Structural Analysis, Cadmium adsorption
Received: 04 May 2025; Accepted: 22 Jul 2025.
Copyright: © 2025 Pang, Li, Yuan, Wei, He, Li, Qiu, Dong and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xin Pang, Shandong University (Qingdao), Qingdao, China
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