ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1623052
This article is part of the Research TopicMetagenomic Insights into Microbial Communities in Fruits and Vegetable PlantsView all 4 articles
Alpine Radish Rhizosphere Microbiome Assembly and Metabolic Adaptation Under PBAT/PLA Humic Acid Biodegradable Mulch Films
Provisionally accepted
- 1Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
- 2Institute of Sichuan Edible Fungi, Chengdu, China
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Alpine agroecosystems present unique crop production challenges due to extreme environmental conditions, where rhizosphere microbiomes significantly influence plant adaptation. To investigate mulch-induced microbial changes in high-altitude agriculture, this study analyzed a radish field in China using SMRT sequencing (16S rRNA/ITS) and metagenomics, comparing PBAT/PLA biodegradable films with/without humic acid (HA) at varying thicknesses. Results demonstrated that radish cultivation selectively enriched Proteobacteria and Acidobacteriota while depleting Chloroflexi and Actinobacteria, with fungal communities shifting from Basidiomycota-to Ascomycota-dominance. Notably, HA-amended mulches enhanced bacterial diversity and specifically promoted polymer-degrading microbes (Chitinophagaceae, Candidatus_Udaeobacter, Chaetomiaceae). Metagenomic profiling revealed thickness-dependent increases in functional genes related to carbohydrate and amino acid metabolism in HA-treated soils. These findings advance our understanding of how biodegradable mulch formulations can be optimized to enhance microbial ecosystem services in alpine farming systems.
Keywords: Alpine agriculture, Biodegradable film, Rhizosphere microbiome, metabolic adaptation, Humic acid
Received: 05 May 2025; Accepted: 24 Jun 2025.
Copyright: © 2025 Zhong, Li, Liao, Ma, Li, Yang, Chang and Miao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mingjun Miao, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
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