- 1Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, Luzhou, China
- 2People’s Hospital of Xuyong County, Luzhou, China
- 3Hospital-Acquired Infection Control Department, The Affiliated Hospital of Southwest Medical University, Luzhou, China
Introduction: Klebsiella pneumoniae is a critical ESKAPE pathogen that presents a significant challenge to public health because of its multidrug-resistant strains. This study investigates the impact and mechanisms of hospital wastewater on the horizontal gene transfer of carbapenem resistance genes, particularly blaIMP, in K. pneumoniae.
Methods: LB broth was prepared using sterile filtered wastewater as the substrate to investigate the impact of wastewater on the transfer of carbapenem-resistant gene blaIMP in K. pneumoniae. The mechanisms of sewage effects on the horizontal transfer of blaIMP were explored by integrating transcriptome sequencing with the detection of extracellular membrane permeability, intracellular reactive oxygen species (ROS), and other test results.
Results: Hospital wastewater significantly enhances the conjugation frequency of plasmids containing blaIMP, showing a two-fold increase in wastewater-based LB broth compared to regular LB broth. In comparison to regular LB broth culture, the wastewater-based LB broth culture group showed significant alterations in the expression of 1,415 genes, with 907 genes upregulated and 508 genes downregulated. Genes related to conjugation transfer systems and the type IV secretion system were significantly upregulated, indicating a potential role in promoting plasmid transfer. Moreover, the treatment of wastewater resulted in elevated intracellular ROS production and increased permeability of bacterial outer membranes, potentially facilitating the spread of antibiotic resistance genes.
Discussion: This research shows that hospital wastewater facilitates the transfer of drug-resistant plasmids containing blaIMP and elucidates its potential mechanisms. A more detailed investigation into these mechanisms may facilitate the prevention of resistance transmission between healthcare and environmental contexts and inform future strategies for managing carbapenem resistance.
1 Introduction
Klebsiella pneumoniae is a part of the ESKAPE pathogens group, which also include Enterococcus spp., Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. This bacterium is known to cause both community-acquired and hospital-acquired infections, such as pneumonia, urinary tract infections, hepatobiliary infections, and sepsis (Martin and Bachman, 2018). In recent years, there has been an increase in the detection of multidrug-resistant K. pneumoniae (Jalal et al., 2023). Of the estimated 4.95 million deaths related to bacterial antimicrobial resistance in 2019, 1.27 million were directly connected to the disease (Antimicrobial Resistance Collaborators, 2022). Due to the limited options for antibiotic treatment and the high mortality rate linked to multidrug-resistant Klebsiella pneumoniae, there is a serious threat to human health on a global scale (Holt et al., 2015).
Carbapenems were once seen as a last resort for treating multidrug-resistant Gram-negative bacterial infections in critically ill patients. However, the widespread use of carbapenems has led to the emergence and spread of carbapenem-resistant K. pneumoniae (CRKP) on a global scale, presenting new challenges to public health security (Navon-Venezia et al., 2017; Cassini et al., 2019). Carbapenemases are the most common mechanism of resistance to carbapenem antibiotics in bacteria. They can be classified into Class A serine carbapenemases, Class B metallo-β-lactamases, and Class D OXA-48-type serine carbapenemases according to the Ambler classification (Queenan and Bush, 2007; Jeon et al., 2015). KPC, which is encoded by blaKPC, is the most common carbapenemase in Class A and the primary resistance mechanism of CRKP to carbapenem antibiotics. NDM, IMP, and VIM are the representative class B metallo-β-lactamases. In China, the KPC-type enzyme is the predominant carbapenemase, while the NDM-type enzyme comes in second and the detection rate of IMP-type enzyme is relatively low (Zhang et al., 2018).
In recent years, there has been an increase in the detection rates of metal-lo-β-lactamases such as NDM and IMP in K. pneumoniae due to the widespread transfer of antibiotic resistance genes between bacteria (Kubota et al., 2019; Xu J. et al., 2020). However, K. pneumoniae carrying blaIMP results in lower levels of carbapenem resistance, which can be easily overlooked, leading to underestimation of the presence of IMP in CRKP (Chen et al., 2009). Bacteria can develop resistance through horizontal gene transfer (HGT) due to various external factors, allowing for the evolution of resistance. Plasmid-mediated conjugative transfer is considered the main method of horizontal transfer of antibiotic resistance genes (ARGs) between bacteria (San Millan, 2018; Lerminiaux and Cameron, 2019). Studying the mechanisms of plasmid-mediated conjugative transfer will help to develop corresponding intervention measures to curb the horizontal transfer of ARGs between bacteria.
Researchers have increasingly recognized the role of the environment in the spread of bacterial resistance in recent years. High levels of microbial pathogens, antibiotics and related metabolites, metals, and resistant bacteria that may carry ARGs in hospital wastewater collectively form a complex environmental reservoir of bacteria (Katagiri et al., 2021; Svebrant et al., 2021). The complex microbial environment and high genetic diversity facilitate the horizontal transfer of ARGs between bacteria through HGT (Zhang K. et al., 2021). An increasing number of researchers have been investigating the effects of various pollutants in the environment, such as biocides residues (Zhang et al., 2023), paclitaxel and its derivatives (Yang et al., 2022), phthalates (Wu et al., 2023), heavy metal ions (Palm et al., 2022), and residual disinfectants (He et al., 2022), on HGT. It has been found that these pollutants can promote the horizontal spread of resistance genes in the environment, with changes in cell membrane permeability, oxidative stress, and the expression of genes related to conjugation playing critical roles. However, most of these studies focus on specific components of pollutants, and there are few reports using real-world wastewater to study gene-level transfer.
Higher concentrations of organic matter, microplastics, and antibiotics in sewage have been shown to interact with a large number of microorganisms, which in turn promotes the production and spread of ARGs (Zhao et al., 2024). Brown et al. explored the impact of sewage-related antibiotics on the development of antibiotic resistance, and their findings supported the idea that changing levels of antibiotics in sewage are linked to horizontal gene transfer of ARGs (Brown et al., 2024). Recently, Basiry et al. (2025) discovered that antibiotics at sub-minimum inhibitory concentrations enhanced the spread of ARGs among the bacterial community in activated sludge sequencing batch reactors in the laboratory. Studying HGT through actual sewage has emerged as a popular research area in the transmission of ARGs. This research utilizes transcriptomic sequencing, along with outer membrane permeability testing and intracellular reactive oxygen species detection in bacteria, to investigate the potential mechanisms of horizontal transfer of the carbapenem resistance gene blaIMP facilitated by wastewater in K. pneumoniae. The goal is to establish a theoretical foundation for preventing the dissemination of resistant bacteria and resistance genes in wastewater, as well as to provide a scientific basis for managing the continued progression of carbapenem antibiotic resistance.
2 Materials and methods
2.1 The collection and treatment of hospital sewage
Sewage samples were collected from the influent of a sewage treatment plant associated with an affiliated hospital of medical university. Once collected, the sewage samples were centrifuged at 4°C and 4,000 r/min for 10 min. Filtered the supernatant through a disposable sterile filter with a pore size of 0.22 μm, repeating the filtration process three times. The filtered samples were stored in a 4°C refrigerator for future use. The chemical composition of sewage was detected through non-targeted analysis by Hangzhou Yanqu Information Technology Co., Ltd. (Hangzhou, China), using Liquid Chromatograph Mass Spectrometer.
2.2 Culture media and antibiotics
LB broth and LB agar were purchased from Guangdong Huankai Microbial Science and Technology Co., Ltd. (Guangzhou, China). Rifampicin was sourced from Heowns Biotechnology Co., Ltd (Tianjin, China), and meropenem was obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). LB broth was prepared by dissolving 36 g of LB broth dry powder in 1 L of deionized water. LB broth consists of 10 g Peptone, 5 g NaCl, 5 g yeast extract, and 1 g dextrose per 1 L; LB agar includes 15 g agar per 1 L of LB broth. The mixture was heated, agitated frequently, and boiled until the powder was completely dissolved. Subsequently, the medium was sterilized in an autoclave at a temperature of 121°C for 15 min. To prepare wastewater-based LB broth, the supernatant of wastewater was used to instead of deionized water. 1,000 mL of wastewater supernatant was measured using a sterilized screw-cap bottle and pressure cooker. 36 g LB broth dry powder was then weighed and added to the measured wastewater supernatant in the bottle. The bottle was shaken until the powder completely dissolved, resulting in clear and transparent broth. The prepared wastewater-based LB broth was filtered three times using a disposable sterile filter with a pore size of 0.22 μm. The filtered broth was then transferred to a sterile container and stored in a refrigerator at 4°C until ready for use.
2.3 Donor and recipient strains
Utilizing a clinically sourced strain of carbapenem-resistant K. pneumoniae as the donor strain, which harbors an IncN_1 type plasmid containing blaIMP and qnrS1, as well as a plethora of genes linked to conjugation and the type IV secretion system (T4SS) (Supplementary Figure S1). Plasmids belonging to the IncN group are typically known for their wide host range and efficient transmission, making them crucial for the spread of key resistance genes (Feng et al., 2016; Chi et al., 2020). The recipient strain for the experiment was Escherichia coli 600 (rifampicin-resistant) maintained by our laboratory.
2.4 Conjugative transfer experiments
Both the donor and recipient bacteria were inoculated onto Columbia blood agar plates and allowed to culture overnight at 35°C in an incubator. A single fresh colony on Columbia blood agar plates was selected and inoculated into 5 mL of broth. The donor bacteria were cultured in LB broth and wastewater-based LB broth separately, while the recipient bacteria were cultured in LB broth. The cultures were then incubated at 35°C with shaking at 160 r/min for 18–24 h. After incubation, the broth was centrifuged, and the supernatant was removed. The bacterial concentration was adjusted using LB broth to prepare a bacterial suspension. 200 μL of the donor bacterial suspension was mixed with 600 μL of the recipient bacterial suspension in a 1.5 mL sterile EP tube, and the mixture was vortexed to ensure thorough mixing. The mixture was then placed in a 35°C incubator for static culture for 4 h.
After incubation, 100 μL of the conjugation mixture was inoculated on selective LB agar plates containing marker antibiotics (meropenem 2 μg/mL and rifampicin 500 μg/mL). Plates were incubated at 35°C for 24 h. The presumed conjugants were purified and then validated by MALDI-TOF MS and colony PCR to confirm the successful plasmid conjugation. Primer sequences and amplification condition are shown in Supplementary text S1. The colonies of transconjugants and recipients were then counted, respectively. All treatments were carried out in triplicate. The conjugative transfer frequency is determined by the ratio of transconjugants (CFU/mL) to recipients (CFU/mL).
2.5 Determination of reactive oxygen species and cell membrane permeability
To understand the underlying mechanisms of improved conjugation, it is crucial to explore whether the elevated generation of bacterial reactive oxygen species (ROS) and the subsequent rise in cell membrane permeability act as the main driving factor. The levels of intracellular ROS were assessed by utilizing the ROS assay kit (Biyuntian, China), following the guidelines provided by the manufacturer. Cell membrane permeability was indicated by the fluorescence intensity of propidium iodide (PI) and 1-N-phenylnaphthylamine (NPN) as described in previous studies (Elliott et al., 2020; Yu et al., 2021).
2.6 Transcriptome sequencing and bioinformatic analysis
RNA-seq was conducted to analyze the transcriptional expression levels in the donor under various culture conditions, including sewage matrix LB broth and normal LB broth. Fresh donor bacterial colonies were handpicked from the blood agar plates and introduced into 50 mL of wastewater-based LB broth and standard LB broth. After incubating the samples at 37°C for 16 h on a constant temperature shaker, they were centrifuged at 8,000 r/min for 10 min to harvest the bacterial cells. The sediment was then placed into a sterile 1.5 mL cryovial, rapidly frozen in liquid nitrogen for 5–10 min, and subsequently stored at –80°C for temporary preservation. RNA isolation, rRNA removal, strand-specific cDNA library construction, and sequencing were conducted by Shanghai Major Biomedical Technology Co., Ltd. (Shanghai, China). Transcriptomic bioinformatics analysis and visualization were conducted based on the MajorBio Cloud platform1 and R language. The specific sequencing process, methods, and bioinformatics analysis are detailed in Supplementary text S2.
2.7 Statistical analysis
The Student’s t-test was employed for statistical analysis of the conjugation frequency, outer membrane permeability, and ROS production. A P-value of < 0.05 was considered statistically significant. GraphPad 8.0 was used for data visualization.
3 Results
3.1 Hospital sewage facilitates the horizontal transfer of blaIMP
Non-targeted chemical composition analysis reveals the presence of a rich variety of organic pollutants in the hospital sewage (Supplementary Table S1). Among these pollutant components, Metformin (Guo et al., 2024) and Acesulfame (Yu et al., 2021) are organic pollutants that have been shown to enhance the dissemination of antibiotic resistance through horizontal conjugative gene transfer. In order to examine how real wastewater affects the horizontal transfer of the carbapenem resistance gene blaIMP, the donor strain was initially cultured in LB broth based on wastewater. The changes in conjugation frequency were then observed through a conjugation experiment. The results showed that after culturing in wastewater-based LB broth, the conjugation frequency was approximately twice that of normal LB broth culturing, with a statistically significant difference (P < 0.05, Figure 1). This indicates that wastewater can promote the horizontal spread of ARGs by enhancing the conjugation transfer of plasmids between bacteria.

Figure 1. Differences of conjugation frequency after different broth cultures. LB, Normal LB broth culture; Sewage LB, Sewage substrate LB broth culture; **P < 0.01.
3.2 Influence of hospital sewage on bacterial gene expression profiles
As shown in Figure 1, Sewage substrate LB broth can promote the conjugation transfer between bacteria. Therefore, to investigate the impact of wastewater on conjugation transfer, strains JYKP3 were cultured in both wastewater-based LB broth and normal LB broth for transcriptomic sequencing. DESeq2 was used to identify differentially expressed genes (DEGs) based on the criteria of |log2 FC| > 1 and Padjust < 0.05. The result showed that compared to normal LB broth culture, there were 1,415 genes with significantly altered expression in the wastewater-based LB broth culture group, with 907 genes upregulated and 508 genes downregulated (Figure 2A). Cluster analysis indicated a clear clustering relationship between the two groups of DEGs (Figure 2B). It is suggested that culturing in wastewater-based LB broth results in a distinct gene expression profile in bacteria, potentially facilitating the transfer of plasmids through conjugation.

Figure 2. Differentially expressed genes and their functional enrichment analysis. (A) Volcano plot of differential expression. (B) Clustering heatmap of all DEGs. (C) Bubble plot of GO enrichment analysis of DEGs. (D) Bubble plot of KEGG enrichment analysis of DEGs.
3.3 GO and KEGG enrichment analysis of DEGs
In order to annotate the biological functions of the identified DEGs, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted on these genes individually. The top 20 GO enrichment results showcasing the varying degrees of enrichment of identified DEGs in biological processes, molecular functions, and cellular components under the condition of Padjust < 0.05 (Figure 2C). The cellular components mainly consist of plasma membranes and protein complexes, while the molecular functions primarily involve the activity of proton transporter ATP synthase and proton channel activity. The biological processes include cellular amino acid catabolism, organic acid metabolism, glutamic acid metabolism, carboxylate metabolism, and small molecule catabolism. Notably, the plasma membrane and carboxylate metabolism processes have the highest number of enriched differentially expressed genes, with 204 and 132 DEGs, respectively.
The results of top 20 KEGG enrichment in the condition of Padjust < 0.05 indicate that the identified DEGs are predominantly enriched in ABC transporters, diverse metabolic pathways, and the production of secondary metabolites (Figure 2D). Of these, ABC transporters show the highest enrichment, with 95 DEGs, while pyruvate metabolism follows with 33 DEGs enriched.
3.4 Differential expression of conjugation-related genes after hospital sewage treatment
Based on the initial sequencing data, the genome of donor strain is found to have numerous genes linked to conjugative transfer. The expression levels of genes associated with conjugative transfer were analyzed among the DEGs identified. The results revealed that genes responsible for the conjugative transfer system, such as traA, traB, traC, traK, traH, traF, traE, traW, traU, traN, traL, and traV, were highly expressed in the experimental group (Figure 3A). Additionally, genes encoding the Type IV secretion system, including virB5, virB8, and virB10, as well as flagellum production-related genes gspJ, gspG, gspL, gspO, fimH, fimG, fimF, fimA, ppdA, and ppdB, showed significant upregulation in the experimental group (Figure 3B).

Figure 3. DEGs related to conjugation. (A) Differentially expressed conjugation-related genes; (B) DEGs related to type IV secretion system and pili production; (C) DEGs of extracellular membrane proteins; (D) differential expression of ROS-related genes; (E) differential expression of SOS-related genes.
Nevertheless, genes involved in ROS production and SOS response regulation, along with genes influencing bacterial outer membrane permeability, exhibit distinct expression patterns in the experimental group compared to the control group. The results demonstrate that in the control group, genes encoding outer membrane proteins such as ompN, exbB, and exbD are highly expressed, whereas in the experimental group, ompW and ompF exhibit high levels of expression (Figure 3C). The gene rpoS, which is associated with oxidative stress, is highly expressed in the experimental group, while the genes soxS and soxR are expressed in the control group (Figure 3D). Additionally, genes involved in regulating the SOS response, such as dinI, dinG, dinB, radA, uvrY, uvrC, uvrA, uvrB, and umuC, are downregulated in the experimental group, while recD, recG, sulA, and ureC are significantly upregulated in the experimental group (Figure 3E). The different expression patterns of these genes with similar functions may be linked to changes in the metabolic state of bacteria in the wastewater matrix LB broth, as indicated by the enrichment of differentially expressed genes in metabolic and secondary metabolite biosynthesis processes.
3.5 Hospital sewage leads to an increase in the permeability of bacterial outer membranes
To investigate the role of outer membrane permeability in promoting conjugative transfer of plasmids in wastewater, this study used NPN and PI analysis to assess changes in bacterial outer membrane permeability after culture in LB broth with wastewater matrix and direct wastewater treatment. Both assays revealed a notable rise in bacterial outer membrane permeability following culture in LB broth with the wastewater matrix and undergoing direct wastewater treatment, with a statistical significance (P < 0.05, Figure 4). The enhanced permeability of the outer membrane in bacteria allowed for easier conjugative transfer of plasmids between them, ultimately aiding in the horizontal dissemination of antibiotic resistance genes during the wastewater treatment process.

Figure 4. Permeability of outer membrane of bacterial. (A) NPN detection of the effect of LB broth culture on membrane permeability in wastewater substrate; (B) PI detection of the effect of LB broth culture on membrane permeability in wastewater substrate; (C) NPN detection of the impact of direct treatment of wastewater on bacterial membrane permeability; (D) PI detection of the impact of direct treatment of wastewater on bacterial membrane permeability. LB, Normal LB broth culture; Sewage LB, Sewage substrate LB broth culture; *P < 0.05, ***P < 0.001.
3.6 Effect of hospital sewage on the production of intracellular ROS in bacteria
The accumulation of intracellular ROS is considered to be one of the key mechanisms by which environmental organic pollutants impact bacterial conjugative transfer. Studies conducted previously have revealed that hospital wastewater samples are filled with various organic pollutants like drug residues, drug metabolites, and organic solvents (Verlicchi et al., 2015). The results of transcriptome sequencing revealed distinct expression patterns of genes associated with oxidative stress in the experimental group. As a result, this study further investigates the mechanisms associated with plasmid conjugative transfer facilitated by wastewater by observing changes in intracellular ROS levels after incubation in LB broth supplemented with a wastewater matrix and direct exposure to wastewater.
Initially, bacterial passage culture was carried out using regular LB broth and LB broth derived from wastewater, which led to the isolation of strain KP21 (cultured in regular LB broth) and strain KP27 (cultured in wastewater-based LB broth). Following treatment with wastewater and PBS, the alterations in intracellular ROS levels between the two strains, KP21 and KP27, were compared. The results indicate that while there was no notable disparity in intracellular ROS levels after 10 min of treatment, a significant difference emerged as the treatment duration extended (Figures 5A,B). Specifically, in the PBS treatment group (Figures 5A,C), there was no significant change in intracellular ROS levels over time in KP21; however, in KP27, intracellular ROS levels showed a gradual increase. The wastewater treatment group (Figures 5B,D) displayed a considerably higher increase in intracellular ROS in KP27 compared to KP21. This indicates that strains passaged in wastewater-based LB broth continue to exhibit higher levels of intracellular ROS even after the removal of external stimuli. Moreover, the strain that underwent passages in LB broth derived from wastewater exhibited a heightened response to external oxidative triggers, as evidenced by the difference in ROS levels between the wastewater treatment and PBS treatment groups (Figure 5E).

Figure 5. The effect of different processing methods on bacterial ROS. (A) Changes in ROS over time after adding PBS to KP21 and KP27; (B) Changes in ROS over time after adding wastewater to KP21 and KP27; (C) ROS detection results after 10 min of adding PBS to KP21 and KP27; (D) ROS detection results after 10 min of adding sewage to KP21 and KP27; (E) The ROS detection results of wastewater treatment minus PBS treatment change over time. KP21, Normal LB broth passage; KP27, Sewage substrate LB broth passage; ns, no significant difference.
Upon the addition of thiourea, a strong reducing agent, to the experimental system, it was observed that there was no significant alteration in ROS levels in either KP21 or KP27 in the PBS treatment group (P > 0.05, Figure 6). Nevertheless, in the wastewater treatment group, thiourea managed to lower the ROS levels triggered by wastewater stimulation, albeit a notable difference persisted compared to the PBS treatment group (P < 0.05, Figure 6). The results of ROS detection in bacteria (KP21, KP27) after being cultured in various broths and subjected to different experimental conditions are summarized in Figure 7. Following the addition of thiourea, there was no change in intracellular ROS levels over time in both the PBS and wastewater treatment groups. Nevertheless, the intracellular ROS levels in the wastewater treatment group were consistently higher than those in the PBS treatment group.

Figure 6. The effect of sewage on bacterial ROS. (a) The effect of sewage on ROS of bacteria passaged by normal LB broth. (b) The effect of sewage on ROS of bacteria passaged by sewage-based LB broth. KP21: Normal LB broth passage; KP27: Sewage-based LB broth passage. The final concentration of thiourea is 100 mmol/L. The difference between different lowercase letters is statistically significant (P < 0.05).

Figure 7. Results of bacterial ROS after passage in different broth. KP21, Normal LB broth passage; KP27, Sewage substrate LB broth passage. The final concentration of thiourea is 100 mmol/L.
4 Discussion
Horizontal transfer of ARGs between bacteria plays a crucial role in the swift advancement of bacterial antibiotic resistance. The “One Health” concept has expanded the spread of ARGs beyond hospital settings, establishing an interconnected transmission network involving humans, animals, and the environment (Kim and Cha, 2021; Zhuang et al., 2021). Monitoring data from “One Health” reveals that the abundance of ARGs in hospital wastewater surpasses that in domestic wastewater by 2–3 orders of magnitude, while showing a homology of over 89% with clinical isolates (Cai et al., 2021). Within the hospital sewage system, there is a unique microenvironment that contains sub therapeutic levels of antibiotics, heavy metal pollutants, and drug-resistant strains, making it an ideal breeding ground for the evolution and dissemination of ARGs (Hassoun-Kheir et al., 2020).
Following the findings by Qiu et al. (2012) regarding the ability of nano-sized aluminum oxide particles in wastewater to facilitate the horizontal transfer of plasmid-mediated ARGs, researchers are now exploring the impact of various pollutants that could be present in wastewater on the transfer of ARGs. Wastewater is a complex integrated system, consisting of a high level of microbial pathogens, drugs and related metabolites, heavy metal ions, and other pollutants, collectively forming a complex environmental reservoir of bacteria (Katagiri et al., 2021; Svebrant et al., 2021). The final biological response in wastewater is frequently the outcome of a blend of various substances. However, it is still unknown whether this combined effect is in line with results from laboratory research. Due to the limitations of experimental conditions and techniques, the majority of studies tend to concentrate on just one or a few pollutant constituents in wastewater, with only a small number of reports utilizing simulated sewage to investigate the horizontal transfer of drug resistance genes.
In this study, wastewater-based LB broth was prepared based on the sterile filtrate of wastewater from the influents of a hospital wastewater treatment plant, and it was found that wastewater-based LB broth significantly enhanced the horizontal transfer of a drug-resistant plasmid containing blaIMP–4 compared to regular LB broth culture. Transcriptomic sequencing analysis showed distinct gene expression patterns in the two broth culture conditions, with 907 genes upregulated and 508 genes downregulated. The differentially expressed genes were predominantly enriched in biological pathways related to the formation of plasma membrane, metabolism of carboxylic acid, ABC transporters, and pyruvate metabolism.
The mating pair formation system and DNA transfer and replication system play a crucial role in the regulation of plasmid conjugative transfer (Miyakoshi et al., 2020). It has been found that exogenous stress or stimulation can affect the frequency of plasmid conjugative transfer by altering the expression of these related genes (Xu L. et al., 2020; Zhang H. et al., 2021). The expression of genes related to the conjugation transfer system in donor bacteria, including traA, traB, traC, traK, traH, traF, traE, traW, traU, traN, traL, and traV, were significantly upregulated after cultivation in the wastewater-based LB broth. Despite the fact that these genes are not all identified on the plasmid containing blaIMP–4 according to the sequencing information, researches have shown that the co-integration of plasmids plays a crucial role in bacterial genetic diversity and the horizontal transmission of ARGs (Li et al., 2020; Liu et al., 2021). Although some R plasmids do not contain genes for conjugation transfer, they can still overcome transmission barriers by working with conjugation plasmids to facilitate the horizontal transfer of ARGs (Li et al., 2020). Thus, the increased expression of these genes strengthens the conjugation system, which is essential for the transfer of drug-resistant genes. In summary, hospital sewage has the potential to enhance the conjugation transfer of plasmids containing blaIMP–4 by influencing the expression of genes involved in conjugation transfer, like traB, traC, and traK.
The T4SS is a multifunctional transport system found in bacteria that plays a crucial role in transferring ARGs between bacteria (Ilangovan et al., 2015; Bergé et al., 2017). It not only transports cellular effector proteins and toxins but also facilitates HGT by either conjugative transfer or delivering genetic material to recipient cells. The T4SS found in Gram-negative bacteria consists of 12 structural proteins, specifically VirB1 to VirB11 and VirD4 (Bergé et al., 2017; Waksman, 2019). According to the transcriptomic analysis conducted in this study, the wastewater-based LB broth culture group exhibited a significant upregulation in the expression of virB5, virB8, and virB10. Despite the fact that not all T4SS encoding genes experienced an upregulation, the significantly upregulated virB10 plays a key role in the outer membrane core complex of T4SS. Its main function is to form an outer membrane channel that connects the inner and outer membranes of bacteria (Costa et al., 2021). Consequently, these T4SS structural genes that are expressed differently may be key players in enhancing plasmid conjugative transfer in wastewater.
Additionally, a large number of flagellum-related genes were found to be upregulated in the wastewater-based LB broth culture group through transcriptomic sequencing. In the investigation of how environmental fungicides affect the transfer of drug-resistant plasmids, Zhang et al. (2023) observed that flagellum-related genes like fimA, ppdA, and ppdB were upregulated to varying degrees and might have a role in plasmid conjugation transfer, which is in line with our research outcomes. While not all flagella are associated with conjugative transfer, the presence of additional flagella can lead to bacteria aggregating or forming biofilms on surfaces. According to the latest study led by Element et al. (2023), it has been discovered that the growth of biofilms significantly elevates the risk of conjugative transfer of carbapenem resistance plasmids in K. pneumoniae. This occurs without the necessity for adaptive costs or changes in transcription, highlighting the importance of biofilms in the propagation of ARGs. Therefore, these flagellum-related genes that are expressed at high levels could potentially boost plasmid conjugative transfer by facilitating bacterial cell aggregation or biofilm formation.
The successful transfer of plasmids between bacteria is dependent on various physiological and biochemical conditions. Factors like intracellular ROS production, the SOS response, and outer membrane permeability all contribute to the efficiency of plasmid conjugative transfer (Wang et al., 2019). A variety of studies have shown that genes with similar functions do not always exhibit the same expression patterns under identical conditions (Zhou et al., 2021; Yu et al., 2023; Guo et al., 2024). In the same vein, our findings also revealed that genes with similar functions exhibited varying expression profiles within the experimental group. ROS can directly target bacterial DNA, proteins, or cell membranes, leading to cell damage, altering gene and protein expression, and ultimately impacting the transfer of ARGs among bacteria (Winterbourn, 2008; Li and Zhang, 2022). Even though the analysis of differential gene expression revealed an increase in rpoS expression and a significant decrease in soxS and soxR expression following treatment with wastewater-based LB broth, the monitoring of intracellular ROS production and its temporal changes, along with the trend of intracellular ROS levels after the addition of the potent reducing agent thiourea, demonstrated a substantial enhancement in intracellular ROS production due to the sewage treatment.
When environmental factors directly target the cell membrane or induce an elevation in intra-bacterial ROS levels, these ROS can in turn damage the cell membrane. This damage increases the permeability of the bacterial cell membrane, allowing for the easier transfer of ARGs across the membrane and thereby promoting the horizontal transfer of ARGs between bacteria (Li and Zhang, 2022). Studies by Zhang et al. (2023) have found that after exposure to biocides, bacterial outer membrane permeability increases, and genes encoding outer membrane proteins such as ompA, ompC, and ompF are upregulated. Similar conclusions were also drawn by He et al. (2022) based on research on disinfectant residues. During this investigation, there was a notable increase in outer membrane permeability observed when culture in wastewater-based LB broth and direct treatment of bacteria with wastewater, as detected by NPN and PI assays. However, the expression of outer membrane protein-encoding genes ompN was downregulated in the experimental group, which may be related to the metabolic state of the bacteria in wastewater. Even though ompN expression was downregulated, outer membrane permeability still increased, potentially because of the elevated expression of outer membrane permeability proteins like ompW and ompF. Additionally, the SOS response may also alter cell membrane permeability and DNA expression (Li and Zhang, 2022). It is hypothesized that sewage may cause direct damage to cell membranes or indirectly lead to increased membrane permeability by triggering SOS response and the production of ROS, ultimately facilitating the transfer of ARGs through plasmids.
In general, our research confirms the potential role of hospital wastewater in the transmission of drug resistance in humans, animals, and the environment. This offers a fresh perspective on managing bacterial resistance within the framework of “one health.” Nevertheless, it is important to acknowledge the limitations of this study. Variations in the composition of pollutants in hospital wastewater across different regions and seasons could result in significant differences in the transfer of ARGs. Moreover, further research is needed to explore the roles and interactions of multiple pollutant components in wastewater in relation to the HGT of drug-resistant genes.
5 Conclusion
In conclusion, hospital wastewater plays a role in the spread of resistance plasmids containing blaIMP–4 by influencing gene expression linked to conjugation, boosting the secretion systems and pili production, and increasing ROS production and bacterial outer membrane permeability. Studying these mechanisms further can aid in preventing resistance transmission between healthcare and environmental contexts, and guide efforts to manage carbapenem resistance moving forward.
Data availability statement
The original datasets presented in the study are publicly available. This data can be found here: https://www.ncbi.nlm.nih.gov/, PRJNA1293672.
Author contributions
YJ: Data curation, Methodology, Conceptualization, Writing – original draft. LS: Conceptualization, Data curation, Writing – original draft. HW: Writing – original draft, Conceptualization, Data curation. YW: Software, Conceptualization, Writing – original draft, Data curation, Methodology. SL: Software, Writing – original draft. CY: Validation, Conceptualization, Writing – original draft. LC: Conceptualization, Writing – original draft, Visualization. ZZ: Writing – review and editing, Validation. JL: Conceptualization, Writing – review and editing, Methodology.
Funding
The author(s) declare that financial support was received for the research and/or publication of this article. This study was supported by the Science and Technology Support Project of Sichuan Province (22ZYZF0007 and 2023YFQ0096), the Luzhou Science and Technology Program (2024RQN225 and 2024RCM246), and the Key Science and Technology Plan of Luzhou City (2023SYF138 and 2023SYF135).
Acknowledgments
We thank home for researchers (www.home-for-researchers.com) for help.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Generative AI statement
The authors declare that no Generative AI was used in the creation of this manuscript.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2025.1626123/full#supplementary-material
Abbreviations
ARGs, antibiotic resistance genes; DEGs, differentially expressed genes; HGT, horizontal gene transfer; NPN, 1-N-phenylnaphthylamine; PI, propidium iodide; ROS, reactive oxygen species; T4SS, type IV secretion system.
Footnotes
References
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Keywords: hospital wastewater, HGT, ARGS, conjugation, Klebsiella pneumoniae
Citation: Jiang Y, Shu L, Wen H, Wei Y, Liu S, Ye C, Cheng L, Zeng Z and Liu J (2025) Enhancement of blaIMP-carrying plasmid transfer in Klebsiella pneumoniae by hospital wastewater: a transcriptomic study. Front. Microbiol. 16:1626123. doi: 10.3389/fmicb.2025.1626123
Received: 10 May 2025; Accepted: 02 July 2025;
Published: 28 July 2025.
Edited by:
Xia Cai, Fudan University, ChinaReviewed by:
Guilherme Sgobbi Zagui, University of Ribeirão Preto, BrazilNafiu Abdulkadir, Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Germany
Copyright © 2025 Jiang, Shu, Wen, Wei, Liu, Ye, Cheng, Zeng and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jinbo Liu, bGl1amI3MjAzQHN3bXUuZWR1LmNu