ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Virology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1627509
Development of a Yeast-based CRISPR Genome Editing System for Feline Coronavirus
Provisionally accepted
- 1The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, China
- 2The Basic Department, College of Veterinary Medicine, China Agricultural University, Beijing, China
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Feline infectious peritonitis (FIP), caused by feline coronavirus (FCoV), is a highly lethal disease characterized by systemic organ infection in cats. Current challenges of FIP include the absence of definitive diagnostic criteria, effective vaccines, and targeted therapies. Developing a robust genome editing toolkit is therefore critical to unraveling FCoV replication and pathogenesis mechanisms, elucidating viral protein functions, and identifying promising diagnostic and therapeutic targets. In this study, we developed a yeast-based CRISPR genome editing system compatible with a TAR-generated FCoV infectious clone, enabling gene deletion, gene insertion, and point mutation with high efficiencies and accuracies. This system not only will contribute to a better understanding of the pathogenic mechanisms of FCoV but also serves as a valuable platform for vaccine development. Furthermore, it offers a possible strategy for genome editing and reverse genetics for other coronaviruses.
Keywords: Feline coronavirus, Feline Infectious Peritonitis, CRISPR, Tar, Reverse Genetics, Virus rescue, Yeast artificial chromosome
Received: 12 May 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Zhang, Zhu, ZHANG and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Di ZHANG, The Clinical Department, College of Veterinary Medicine, China Agricultural University, Beijing, China
Yueping Zhang, The Basic Department, College of Veterinary Medicine, China Agricultural University, Beijing, China
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