ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Symbioses
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1630092
¬¬¬Study of the сervical сanal microbiome and microbiocenosis in reproductive-age women with squamous intraepithelial lesion
Provisionally accepted
Анастасия Владимировна Перемыкина1
Valery Cheranev2*
Alexey Y Shilyaev1Andrey Krivoy2Zhanna Repinskaia2Dmitriy Korostin2
Denis V. Rebrikov2- 1Department of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Ministry of Healthcare of the Russian Federation, Moscow, Russia
- 2Center for Precision Genome Editing and Genetic Technologies for Biomedicine , Pirogov Russian National Research Medical University, Moscow, Russia
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The study of the cervicovaginal microbiome is a critical area of research in medical science. According to scientific data, microorganisms inhabiting the lower female genital tract may influence susceptibility to and persistence of human papillomavirus (HPV), as well as the development and progression of squamous intraepithelial lesions (SIL) toward cervical cancer (CC).The study included 67 patients with a histological diagnosis of low-grade squamous intraepithelial lesion (LSIL) and 85 patients with high-grade squamous intraepithelial lesion (HSIL). The cervical canal microbiome of patients with LSIL (n=31) and HSIL (n=45) was examined using the 16S rRNA gene sequencing method, while the microbiocenosis of the remaining patients with LSIL (n=36) and HSIL (n=40) was analyzed using PCR-RT.There are patients with HSIL on average older than patients with LSIL about 3 years. HPV 16 was found to be the most common virus type in patients with SIL. When determining of 16S rRNA genes of prokaryotic microorganisms in women of reproductive age with SIL by the new generation sequencing, it turned out that the cervical canal microbiome is inhabited by many atypical representatives (soil, aquatic and aerobacteria). In addition to the genera Lactobacillus, Streptococcus,
Keywords: Sil, cc, NGS, HPV, bacterial vaginosis, Aerobic vaginitis, lactobacilli, microbiome
Received: 19 May 2025; Accepted: 28 Jul 2025.
Copyright: © 2025 Перемыкина, Cheranev, Shilyaev, Krivoy, Repinskaia, Korostin and Rebrikov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Valery Cheranev, Center for Precision Genome Editing and Genetic Technologies for Biomedicine , Pirogov Russian National Research Medical University, Moscow, Russia
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