Hemotropic pathogens in aborted fetuses of domestic ruminants: Transplacental transmission and implications for reproductive loss

Daria Jurković Žilić¹Šimun Naletilić¹Željko Mihaljević¹Ema Gagović¹Silvio Špičić¹Irena Reil¹Sanja Duvnjak¹Maja Zdelar Tuk¹Adnan Hodzic²Relja Beck^1*

• ¹Croatian Veterinary Institute, Zagreb, Croatia
• ²Division of Terrestrial Ecosystem Research, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Vienna, Austria

Objective: Hemotropic pathogens of the genera Anaplasma, Babesia, Theileria, and hemotropic Mycoplasma are significant infectious agents in domestic ruminants, most commonly associated with vector-borne transmission. However, their potential for transplacental transmission and their contribution to reproductive disorders remains poorly understood. This study aimed to investigate the presence of hemopathogens in aborted fetuses of cattle, sheep, and goats in Croatia, and to evaluate their potential role in transplacental transmission.Methods: Molecular analyses were conducted on tissue samples from 651 aborted fetuses collected between 2016 and 2019 as part of national abortion surveillance programs. PCR screening followed by sequencing were used to detect Anaplasmataceae, Babesia, Theileria, and hemotropic Mycoplasma.Results: Thirteen hemopathogens were detected in 94 of 651 fetuses (14.44%), including Anaplasma marginale, A. ovis, A. phagocytophilum, Theileria orientalis, T. ovis, Theileria sp. OT3, Babesia ovis, B. canis, B. vulpes, Mycoplasma wenyonii, M. haemobos, M. ovis, and M. haemominutum. The highest infection rates were observed in cattle (17.27%) and sheep (15.85%), while goats showed significantly lower prevalence (5.3%). A. marginale and A. ovis were the most frequently detected pathogens in bovine and ovine fetuses, respectively. Hemotropic mycoplasmas were reported for the first time in Croatia, with the first Western Balkan record of 'Candidatus M. haemobos'. Our study represents the first molecular documentation of a wide array of hemopathogens in aborted ruminant fetuses in Croatia, strongly indicating the possibility of transplacental transmission. The detection of species-specific patterns and the unexpected identification of protozoan species typically associated with canines highlight complex epidemiological dynamics.Vertical transmission of the detected pathogens may play a role in abortion in endemic regions and should be integrated into differential diagnostic protocols for reproductive failure investigations.