ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1633245
This article is part of the Research TopicMaterials, Adaptation, and Evolution: How Anthropogenic Activities Impact Microbial ResistanceView all 3 articles
Dual function of Desmidorchis retrospiciens -based gold nanoparticles as antibacterial and osteoinductive agent for managing osteomyelitis
Provisionally accepted
- 1King Faisal University, Al Ahsa, Saudi Arabia
- 2King Salman Center for Disability Research, Riyadh, Saudi Arabia
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Osteomyelitis is a chronic inflammation of bone due to pathogenic infection mainly by methicillinresistant Staphylococcus aureus (MRSA). Osteomyelitis can cause severe bone damage and pathologic fractures, which subsequently resulted in high physical disability rate. Thus, a therapeutic agent with antibacterial and bone regenerative activities can be effective in the management of osteomyelitis.We phyto-fabricate gold nanoparticles using plant extract of Desmidorchis retrospiciens (DR-AuNPs) and examined their antibacterial and osteoinductive activities. The biosynthesis of DR-AuNPs confirmed with several characterization techniques including scanning and transmission electron microscope, X-ray diffraction (XRD), Energy dispersive x-ray analysis (EDX), and Fourier transform infrared spectroscopy (FTIR). Biofilm assays and reactive oxygen species (ROS) examination used to examine the antibacterial effect of DR-AuNPs. In vitro and ex-vivo cell biology assays used to study the osteoinductive effect of DR-AuNPs.Results: DR-AuNPs displayed substantial antibacterial activity against the MRSA-induced osteomyelitis via increasing ROS production, membrane leakage, membrane permeability, and disruption of surface structure in the MRSA. Several anti-biofilm assays and electron microscopic studies established the efficacy of DR-AuNPs, to significantly inhibit the formation of MRSA biofilm. Interestingly, DR-AuNPs showed to promote the lineage commitment of bone-marrow derived mesenchymal stem cells (BMSCs) into osteoblasts in vitro and to enhance their ectopic bone formation capacity in vivo without causing any cell toxicity. Discussion: Our data provide green DR-AuNPs with dual antibacterial and osteoinductive activities as a promising bioactive agent for management of osteomyelitis.
Keywords: gold nanoparticles, Disability, Osteomyelitis, Osteoinductive, Antibacterial, MRSA
Received: 22 May 2025; Accepted: 21 Jul 2025.
Copyright: © 2025 Abdallah, Rajendran and Ali. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Basem M Abdallah, King Faisal University, Al Ahsa, Saudi Arabia
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