ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Systems Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1634775
Six new bacterial species of Marinilabiliales isolated from the marine coastal sediment and reclassified Ancylomarina and Labilibaculum as Marinifilum comb. nov. based on the genome analysis
Provisionally accepted
- Shandong University, Jinan, China
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Evaluation of bacterial succession with cultivation-dependent strategies during enrichment culturing marine sediment led to the isolation of 6 strains that affiliated with the order Marinilabiliales. Six strains were selected for a taxonomic study after discarding clonal cultures. A thorough phylogenetic, genomic and phenotypic analysis of the isolates indicated that they represented six new species. Molecular data revealed the existence of an as yet uncultivated novel species recurrently binned from the enrichment culturing metagenomes. Using a combination of genomic, phylogenetic, and biochemical approaches, we characterized six novel Marinilabiliales species capable of degrading marine macroalgal polysaccharides. Bioinformatic Polysaccharide Utilization Loci (PUL) annotations suggest usage of a large array of polysaccharides, including laminarin, α-glucans, and alginate as well as mannans and fucans, highlighting the genus' involvement in the marine carbon cycle. This study represented a new example of the use of the tandem approach of whole cell mass spectrometry linked to 16S rRNA gene sequencing in order to facilitate the discovery of new taxa by high-throughput cultivation, which increases the probability of finding more than a single isolate for new species. Analysis of CAZymes genes and PUL counts revealed substantial potential for polysaccharide utilization of Marinilabiliales. The taxonomic study resulted in the classification of six new species and reclassified Ancylomarina and Labilibaculum as Marinifilum of the order Marinilabiliales for which we propose the names Carboxylicivirga agarovorans sp. nov., Carboxylicivirga longa sp. nov.,
Keywords: Marinilabiliales1, Polysaccharide Utilization Loci2, Coastal marine sediments3, Carbohydrate-active enzymes4, Genome size5, Average amino acid identity6, Percentage of conserved proteins7 Taxonomic study of Marinilabiliales and Proposal of Carboxylicivirga agarovorans sp. nov., Carboxylicivirga longa sp. nov.
Received: 25 May 2025; Accepted: 30 Jun 2025.
Copyright: © 2025 Zhang, Teng, Zhou, Liu, Lu and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dechen Lu, Shandong University, Jinan, China
Zong-Jun Du, Shandong University, Jinan, China
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