ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Antimicrobials, Resistance and Chemotherapy
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1636121
Isolation, Identification, and Characterization of a Marine Lactiplantibacillus plantarum Strain with Antimicrobial Activity against Burkholderia contaminans
Provisionally accepted
- 1Jimei University, Xiamen, China
- 2Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China
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Burkholderia contaminans, an opportunistic pathogen commonly found in the food and cosmetics industries, has serious potential to cause severe human infections and industrial contamination. However, compared to traditional physical or chemical antimicrobial treatment, the novel biological antimicrobial strategies against B. contaminans has not been extensively explored. In this study, a strain with antimicrobial activity against B. contaminans was isolated from a marine grouper aquaculture pond and identified as Lactobacillus plantarum Dys01. The antimicrobial activity of L. plantarum Dys01 mainly originated from its metabolites, with a minimum inhibitory concentration (MIC) of 8 mg/mL. Component analysis indicated that the antibacterial substances of L. plantarum Dys01 primarily included organic acids, proteinaceous substances, and hydrogen peroxide, among which organic acids and proteinaceous substances played the major inhibitory roles. Additionally, the metabolites of L. plantarum Dys01 significantly inhibited the biofilm formation of B. contaminans in a dose-dependent manner. Alkaline phosphatase activity assays and propidium iodide staining revealed that metabolites produced by L. plantarum Dys01 could disrupt the cell wall and cell membrane integrity of B. contaminans. This was further confirmed by scanning electron microscopy, which showed typical morphological damage such as surface indentations and membrane rupture. Therefore, our study provided novel insights into the control of B. contaminans contamination in the food, cosmetic, and pharmaceutical industries, and laid an important theoretical foundation for the development of novel biopreservatives.
Keywords: marine microorganisms, Burkholderia contaminans, Lactobacillus plantarum, Metabolites, Antimicrobial activity
Received: 28 May 2025; Accepted: 07 Jul 2025.
Copyright: © 2025 Du, Xin, Yang, Sui, Yang, Zeng and Chan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Runying Zeng, Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China
Zhuhua Chan, Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China
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