ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1637383
Combined transcriptome and metabolome analysis reveals the regulatory network of histidine kinase QseC in the two-component system of Glaesserella parasuis
Provisionally accepted
- 1School of Physical education, Southwest Medical University, Luzhou, China
- 2School of Pharmacy, Southwest Medical University, Luzhou, China
- 3Experimental animal center, Southwest Medical University, Luzhou, China
- 4Experimental animal center, Technology Department, Southwest Medical University, Luzhou, China
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Glaesserella parasuis (G.parasuis) causes agent Glässer's disease in swine. This study investigated the mechanism of QseC in G.parasuis with transcriptome and metabolomics sequencing techniques. Transmission electron microscopy revealed that ΔqseC mutant exhibited cell wall dissolution and cytoplasmic rarefaction, indicating membrane homeostasis disruption. Metabolomics analysis identified 819 metabolites, with 24/36 showing significant alterations in positive/negative ion modes. KEGG enrichment indicated abnormalities in amino acid synthesis and quorum sensing. Transcriptomic revealed 663 differentially expressed genes (DEGs), including upregulated membrane synthesis genes (plsB and wecA) and downregulated virulence factors (hrpA and pilW). Integrated analysis demonstrated that plsB and wecA formed association networks with methionine and prostaglandin metabolites. These results establish QseC's global regulatory role in G.parasuis, providing insights for novel control strategies.
Keywords: G.parasuis, two-component system, QseC, Transcriptomics, Metabolomics, Bacterial Pathogenesis
Received: 29 May 2025; Accepted: 11 Aug 2025.
Copyright: © 2025 Yan, Zhou, Liu, Gu, Xiao, Zhao, Yu and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Lvqin He, Experimental animal center, Technology Department, Southwest Medical University, Luzhou, China
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