REVIEW article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1637673
This article is part of the Research TopicThe Role of Gut Microbes and Their Metabolites in Metabolic Diseases: Mechanisms and Therapeutic TargetsView all 9 articles
Laboratory Preparation Methods for Human-Derived Fecal Microbial Suspensions for Fecal Microbiota Transplantation: A Review and Standardization Perspectives
Provisionally accepted
- 1Qingdao University, Qingdao, China
- 2Yantai Yuhuangding Hospital, Yantai, China
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Fecal microbiota transplantation (FMT) has advanced significantly as a therapeutic approach over the past few decades. Preparing fecal suspensions for FMT is one of the key steps. However, there is no unified standard or recognized procedure for preparing fecal suspensions in laboratories.This review evaluated the steps currently employed in laboratories to prepare fecal suspensions for FMT, including sample collection, suspension buffers, homogenization, purification, filtration, centrifugation, cryopreservation, dosage, the operating environment, and the transplantation form.This review focuses on the different operations of each preparation step, aiming to provide a reference for the laboratory preparation of fecal suspensions.
Keywords: fecal microbiota transplantation, fecal suspension preparation, Laboratory preparation, protocol standardization, Microbial Viability, Gut Microbiota diversity
Received: 10 Jun 2025; Accepted: 13 Aug 2025.
Copyright: © 2025 Gong, Liu and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Liuye Huang, Yantai Yuhuangding Hospital, Yantai, China
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