Antibiotic Resistance Patterns of Environmental Bacteria from Sewage Water in Vellore, India: Isolation, Virulence Analysis and Characterization

Surbhi Kumari BarnwalArabi Mohammed Saleh M A^*

• Vellore Institute of Technology, Vellore, India

Globally, the rise of antibiotic resistance is a pressing One Health concern, where environmental sources, particularly wastewater, play a critical role in the dissemination of resistant bacteria. The influx of pharmaceutical waste, likely to contain antibiotics, into the environment would lead to the chronic presence of antibiotics and development of resistance in environmental bacteria. This study aimed to investigate the prevalence and antibiotic resistance patterns of bacterial isolates obtained from sewage receiving hospital wastewater.Sewage samples were collected from 4 different locations in Vellore, Tamil Nadu, India. The samples were further analyzed using LC/MS for quantification of amoxicillin, meropenem and vancomycin. The bacterial isolates were obtained by both direct and enrichment culture techniques. The isolates were phenotypically characterized by analysing the colony morphology and through gram staining, and virulence tests (hemolysis assay, biofilm formation assay, and protease, amylase and lipase activity assays). Minimum inhibitory concentrations (MICs) against amoxicillin, meropenem and vancomycin were determined using both antibiotic gradient strips and broth microdilution methods, following EUCAST guidelines.Molecular identification was performed using 16S rRNA gene sequencing. Although antibiotics were below the limit of quantification in the samples, significant resistance was observed among the isolates. A total of ten bacterial strains, including Stenotrophomonas, Sphingobium, Brucella, Agrobacterium, Ochrobactrum, Acinetobacter, Klebsiella and Pandoraea were identified. Most of the isolates exhibited multidrug resistance (MDR), with notable variability in MIC values (p < 0.05). Pandoraea sp. strain VITSA19 displayed the highest resistance to all the tested antibiotics (≥ 4096 µg/mL for amoxicillin, ≥ 512 µg/mL for meropenem and ≥ 4096 µg/mL for vancomycin). Two isolates, Stenotrophomonas sp. strain VITSA1 and Stenotrophomonas pavanii strain VITSA2, demonstrated hemolysin and protease production. These findings underscore sewage as a reservoir of MDR bacteria and highlight the environmental dimension of antibiotic resistance spread. From a One Health perspective, the study emphasizes the urgent need for integrated environmental antimicrobial resistance (AMR) surveillance and improved wastewater treatment practices to mitigate the risk of resistance transmission to human and ecological health.