ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Food Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1646082
This article is part of the Research TopicNew Insights in Multifunctional Foods: Applications of Postbiotics, Pharmabiotics, Psychobiotics, Parabiotics, and CobioticsView all articles
Lab-Scale Production of Postbiotic Proteins from Bifidobacterium adolescentis with Antiviral and Epithelial-Protective Properties
Provisionally accepted
- 1Pontificia Universidad Javeriana, Bogotá, Colombia
- 2Pontifical Javeriana University, Bogotá, Colombia
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Postbiotics produced by probiotic bacteria are gaining attention as multifunctional, food-derived agents with potential applications in human and animal health. This study investigates the production and biological activity of protein-rich postbiotics from Bifidobacterium adolescentis, cultivated under controlled conditions in a 3-liter bioreactor as a laboratory-scale model for functional ingredient development. Culture parameters were improved, and one representative batch was selected for biological evaluation. The postbiotic preparation was tested for cytotoxicity using MA104 (renal) and C2BBe1 (intestinal) epithelial cell lines through viability and cell death assays, confirming its safety across a range of concentrations. To assess its functional activity, we evaluated its ability to reduce rotavirus infection and preserve epithelial integrity. The postbiotic significantly reduced viral infectivity and maintained cytoskeletal architecture in infected intestinal cells, supporting its potential protective role. These findings suggest that B. adolescentis-derived postbiotics may serve as safe and biologically active compounds with potential applications in intestinal health and viral infection management.
Keywords: Bifidobacterium adolescentis, Postbiotics, bioreactor, Rotavirus, Gutepithelium, Antiviral activity, Cytoskeletal integrity, functional ingredients
Received: 13 Jun 2025; Accepted: 27 Aug 2025.
Copyright: © 2025 Hernandez, Quevedo, Cabrera and ULLOA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: JUAN ULLOA, Pontifical Javeriana University, Bogotá, Colombia
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