ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Terrestrial Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1652881
This article is part of the Research TopicRole of Microbes in One Health: The Interconnectedness of Soil, Plant and Animal Health in Maintaining Ecosystem Services and FunctionsView all articles
Features and Rhizosphere Colonization Strategies of Lactobacillus plantarum 0308 in Soil-Tomato Systems
Provisionally accepted
Xiancui Zhang1*
Haoran Liao1
Tong Cai2Peiwen Cai1Xiangyu Wu1Zhe Wang1Haoyu Ma1Guoqiang Qiu3Mingxing Zhao1Xingmeng Lu4Xianting Wang5Choufei Wu1- 1Huzhou University, Huzhou, China
- 2Shandong Center for Disease Control and Prevention, Jinan, China
- 3Deqing County Ecological Forestry Integrated Service Center, Huzhou, China
- 4Zhejiang University, Hangzhou, China
- 5Hynobius Anji National Nature Reserve, Huzhou, China
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The Lactic acid bacteria (LAB) has shown great potential as a sustainable solution to support agriculture through its plant-growth-promoting and biocontrol activities. However, their efficacy as bioinoculants is limited by unpredictable colonization in natural conditions. The bacterial strain LP0308, identified as Lactobacillus plantarum (LP0308) based on 16S rRNA sequence analysis, was obtained from rhizosphere soil. LP0308 was introduced into the soil of tomato, and it could stably persist and proliferate for a long-term (0-20 days), as confirmed by CFU, RT-qPCR and fluorescence in situ hybridization (FISH) analyses. Further characterization revealed that LP0308 altered the microbial composition of the rhizosphere soil and significantly increased the abundance of Bacillus and potentially pathogenic microorganism. Further analyses revealed that LP0308 altered the rhizosphere soil microbial community, significantly increasing the abundance of Bacillus spp. while decreasing the potential pathogenic microorganisms, such as R. solanacearum, F. oxysporum. In addition, the successful colonization of LP0308 led to drastically increased expression of encoding biofilm (vpsI1, vpsI2, vpsC and vpsI3), immune modulation (pbpG, kdtB, and wbpL) and antimicrobial activitity gene (farB). L. plantarum strain LP0308 was confirmed as a possible plant growth-promoting rhizobacteria (PGPR), which significantly promoted bud length, plant height, primary root length, root fresh weight, and whole-seedling fresh weight. Additionally, application of LP0308 markedly improved soil nutrient availability and stimulated key enzymatic activities. Together, our findings suggest the LP0308 as a potential target for developing more effective bioinoculants for sustainable agriculture.
Keywords: colonization, Lactobacillus plantarum, Microbial composition, rhizosphere soil, Tomato
Received: 24 Jun 2025; Accepted: 12 Aug 2025.
Copyright: © 2025 Zhang, Liao, Cai, Cai, Wu, Wang, Ma, Qiu, Zhao, Lu, Wang and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xiancui Zhang, Huzhou University, Huzhou, China
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