ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1655103
This article is part of the Research TopicMicrobe Empowering Green Agriculture and Boosting Productivity and QualityView all 21 articles
Biocontrol Potential of Newly Isolated Streptomyces noursei D337-11 from Disease Suppressive Soil and Its Metabolites Against Fusarium oxysporum f. sp. cubense in Banana Plants
Provisionally accepted
Dengbo Zhou1
Xinxin He1
Yufeng Chen1
Chunting Li1
Wei Wang1
Zhiqiang Pan2Yankun Zhao1Yongzan Wei1Junting Feng1
Miaoyi Zhang1
Dengfeng Qi1
Xiaojuan Li1Kai Li1
Tao Jing1*
Jianghui Xie1*- 1Institute of Tropical Bioscience and Biotechnology, Haikou, China
- 2USDA-ARS Natural Products Utilization Research Unit, University, United States
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense, threatens global banana production. Biocontrol using Streptomyces is a promising strategy. Here, we identified Streptomyces noursei D337-11 from disease suppressive banana soils, which exhibited dual functionality in controlling Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), achieving a 65.35% suppression of disease, and promoting plant growth in pot experiments. The extracts from this strain restored the soil microbiota disrupted by Foc TR4, particularly, reviving Saccharimonadales populations.Using integrated untargeted metabolomics (LC-ESIMS/MS) and genomic analysis, we identified five bioactive metabolites, including naringenin, a flavonoid linked to a 100% homologous biosynthetic gene cluster (Region 52.1). Genome-guided differential metabolomics established the genetic basis for flavonoid production. Mechanistic characterization indicated that naringenin exhibits antifungal activity via dual-target inhibition: molecular docking showed strong binding to the trypsin of Foc TR4 (N1RZA7, -6.6 kcal/mol) and nitroalkane oxidase (N1R9V2, -8.4 kcal/mol), which disrupts cellular integrity as evidenced by ultrastructural anomalies. We provide the first evidence of Streptomyces-derived naringenin as a multi-target antifungal agent. Overall, this study provides a theoretical basis for exploring the application of microbial flavonoids in biological control of fungal diseases.
Keywords: Streptomyces noursei, Genome-guided differential metabolomics, Banana Fusarium wilt, Naringenin, Target protein
Received: 27 Jun 2025; Accepted: 30 Jul 2025.
Copyright: © 2025 Zhou, He, Chen, Li, Wang, Pan, Zhao, Wei, Feng, Zhang, Qi, Li, Li, Jing and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Tao Jing, Institute of Tropical Bioscience and Biotechnology, Haikou, China
Jianghui Xie, Institute of Tropical Bioscience and Biotechnology, Haikou, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.