ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiotechnology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1670387
This article is part of the Research TopicGreen Biomanufacturing by Industrial Microorganisms: Precise Regulation and System OptimizationView all 4 articles
Efficient butanol bioproduction from renewable lignocellulosic biomass by an integrated strategy of ternary deep eutectic solvent pretreatment and clostridial fermentation: Towards complete utilization of reed straw
Provisionally accepted
- Huaiyin Institute of Technology, Huaiyin, China
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The main route for biobutanol production is anaerobic clostridial fermentation using corn and sugarcane as substrates. The high-price of raw materials in above processes largely limits its application as an advanced biofuel. The renewable lignocellulosic biomass is an ideal feedstock to produce butanol. However, the structure of lignocellulose is complicated which needs pretreatment to facilitate enzymatic efficiency and substrate fermentability. Herein, the green ternary deep eutectic solvent (DES) composed of choline chloride, succinic acid, and glycerol was synthesized. To improve DES pretreatment performance and reduce the viscosity of reaction system, water was added as co-solvent. Results indicate that xylan removal and glucan recovery were 61.7% and 93.6% when pretreating reed straw (RS) by DES/H2O (80:20, wt%) at 120°C for 4 h. In this case, glucose yield reached 87.9% by cellulase after 72 h enzymolysis, which is 6.2-fold to untreated RS. Afterwards, saccharification yield of treated reed was further elevated to 93.1% assisted by Tween 80 (100 mg/g-substrate). The reed hydrolysate was then applied as substrate for biobutanol production by Clostridium acetobutylicum ATCC 824, and approximately 6.5 g/L butanol and 8.8 g/L total solvents was obtained from the real hydrolysate. Finally, the enzymatic residues of reed were prepared to porous hydrochar materials via hydrothermal carbonization at 220°C for 8 h. Adding 2.0 g/L hydrochar was verified for enhancing butanol titer to 11.5 g/L from synthetic medium containing typical pretreatment-derived inhibitors. In summary, this study provides an efficient butanol bioproduction route integrated with the complete valorization of renewable lignocellulosic biomass.
Keywords: green biomanufacturing, Lignocellulose, Enzymatic hydrolysis, butanol fermentation, Clostridium acetobutylicum, Hydrochar
Received: 21 Jul 2025; Accepted: 20 Aug 2025.
Copyright: © 2025 Shao, Zhang, Wang, Xie, Wang, Yang and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Hongzhen Luo, Huaiyin Institute of Technology, Huaiyin, China
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