Transfer of blaTEMGene Between Salmonella and Escherichia Coli under Processing Conditions of Animal Products: Influence of a Copper(II) Complex

Rosanne Aparecida Capanema Ribeiro¹Micaela Guidotti-Takeuchi^1,2Carolyne Ferreira Dumont^1*Roberta Torres de Melo^1*Ana Beatriz Garcez Buiatte¹Bárbara de Araújo Brum¹Thais Jansen Martins¹Wendell Guerra¹Luana Munique Sousa Ramos¹Richard Costa Polveiro¹Daise Aparecida Rossi¹

• ¹Federal University of Uberlandia, Uberlândia, Brazil
• ²Instituto Federal de Educacao Ciencia e Tecnologia Farroupilha, Santa Maria, Brazil

The high prevalence of infections caused by contaminated food, coupled with growing antimicrobial resistance, especially through horizontal gene transfer, is a challenge for public health worldwide. It is possible that this situation is intensified in the presence of by-products from animal product processing industries. In view of this, we investigated the horizontal transfer of the blaTEM gene from S. Heidelberg to E. coli J53 AzR, in the absence and presence of whey (WH) and chicken juice (CJ) in: i) liquid medium for three hours under agitation; ii) solid medium overnight; iii) liquid medium overnight and the influence of the copper(II) complex Lu54 in mitigating this transfer. The first protocol showed the highest relative conjugation frequency (RCF) of 2.23% in the absence of supplements and increased by three and four orders of magnitude in the presence of CJ and WH and was selected for treatment with Lu54. In solid/overnight, there were RCFs of less than 1%, while the liquid/overnight medium showed RCFs higher than the first protocol only in WH. The presence of WH acidified the medium, which resulted in higher RCF. Lu 54 reduced RCF from 2.2 to 0.3%, 8.2 to 1.7% and 6.2 to 0.9%, respectively, for the tests without by-products and with WH and CJ. In addition, the genomes were sequenced to map the blaTEM gene and β-lactamase families in transconjugants. The results showed that three plasmids containing blaTEM were detected in the controls and the same gene was not identified in the treatments, suggesting plasmid loss induced by the copper(II) complex (Lu54). The results prove that WH and CJ increase the frequency of conjugation in liquid media, and the Lu54 complex is a promising alternative to mitigate conjugation and, consequently, the spread of antimicrobial resistance, especially in milk and meat processing industries.