ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbial Physiology and Metabolism
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1678324
A two-component system MechNtrB/MechNtrC related to nitrogen metabolism regulation in Micromonospora echinospora DSM43816
Provisionally accepted
- Jiangxi Normal University, Nanchang, China
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The NtrB/NtrC two-component signal transduction system (TCS), predominantly found in Gram-negative bacteria, plays a crucial role in nitrogen metabolism regulation. Through BLASTP analysis of the complete proteome of Micromonospora echinospora DSM 43816 against the NtrB/NtrC database, homologous proteins were identified and designated as MechNtrB and MechNtrC. Subsequent in vitro expression and phosphorylation assays demonstrated that both proteins undergo phosphorylation, indicating a phosphoryl transfer mechanism for signal transduction between them. Yeast two-hybrid (Y2H) screening using selective media further verified specific in vivo protein-protein interactions between MechNtrB and MechNtrC. Quantitative real-time PCR (RT-qPCR) analyses revealed that overexpression of MechNtrB or MechNtrC gene significantly modulated the expression levels of key nitrogen metabolism genes, including those encoding glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH). These collective findings establish that MechNtrB/MechNtrC constitutes a functional TCS in M. echinospora that directly regulates bacterial nitrogen metabolism.
Keywords: Micromonospora echinospora, nitrogen metabolism, metabolicregulation, two-component system, MechNtrB/MechNtrC
Received: 02 Aug 2025; Accepted: 13 Oct 2025.
Copyright: © 2025 Long, Lu, Leng, Li, Ni, Zou, Wu and Long. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhong-er Long, longzhonger@163.com
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