ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
This article is part of the Research TopicNew and advanced mechanistic insights into the influences of the infant gut microbiota on human health and disease, Volume IIView all 15 articles
Human milk-derived Bifidobacterium longum subsp. infantis CCFM1269 alleviates food allergy by modulating gut microbiota and restoring intestinal barrier
Provisionally accepted
- 1Wuxi Maternity and Child Health Care Hospital, Wuxi, China
- 2Jiangnan University, Wuxi, China
- 3Franklin & Marshall College, Lancaster, United States
- 4Soochow University, Suzhou, China
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Food allergy (FA) is an immune-mediated disorder increasingly linked to intestinal dysbiosis and epithelial barrier dysfunction. This study evaluated the protective effects of Bifidobacterium longum subsp. infantis CCFM1269, a human milk-derived probiotic, using a β-lactoglobulin-induced FA mouse model. Oral administration of CCFM1269 significantly reduced allergic symptoms, including poor weight gain, diarrhea, and elevated allergy scores. The probiotic restored immune balance by downregulating IL-4 and IL-17A and upregulating IL-10 and IFN-γ in both serum and jejunal tissues. It also alleviated oxidative stress by increasing superoxide dismutase activity and decreasing malondialdehyde levels. CCFM1269 enhanced intestinal barrier integrity through the upregulation of tight junction proteins Occludin, Claudin 1, and ZO-1. Microbiome analysis showed that CCFM1269 reshaped the gut microbial structure by increasing Helicobacter and reducing Alloprevotella and Bacteroides, taxa strongly associated with FA severity. Correlation analysis confirmed that these microbial changes were linked to improvements in immune and barrier function. Collectively, these findings indicate that human milk-derived CCFM1269 alleviates FA through microbiota modulation and epithelial restoration, supporting its potential as a probiotic strategy for preventing and treating food allergy.
Keywords: Bifidobacterium longum subsp. infantis, food allergy, Gut Microbiota, Immune Modulation, intestinalbarrier, β-Lactoglobulin
Received: 17 Oct 2025; Accepted: 08 Dec 2025.
Copyright: © 2025 Ran, Yu, Li, Yin, Jiang and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Renqiang Yu
Bo Yang
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