Abstract
Retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA) are inherited degenerative retinal dystrophies with vision loss that ultimately lead to blindness. Several genes have been shown to be involved in early onset retinal dystrophies, including CRB1 and RPE65. Gene therapy recently became available for young RP patients with variations in the RPE65 gene. Current research programs test adeno-associated viral gene augmentation or editing therapy vectors on various disease models mimicking the disease in patients. These include several animal and emerging human-derived models, such as human-induced pluripotent stem cell (hiPSC)-derived retinal organoids or hiPSC-derived retinal pigment epithelium (RPE), and human donor retinal explants. Variations in the CRB1 gene are a major cause for early onset autosomal recessive RP with patients suffering from visual impairment before their adolescence and for LCA with newborns experiencing severe visual impairment within the first months of life. These patients cannot benefit yet from an available gene therapy treatment. In this review, we will discuss the recent advances, advantages and disadvantages of different CRB1 human and animal retinal degeneration models. In addition, we will describe novel therapeutic tools that have been developed, which could potentially be used for retinal gene augmentation therapy for RP patients with variations in the CRB1 gene.
CRB Family Members
Crumbs (Crb) is a large transmembrane protein initially discovered at the apical membrane of Drosophila epithelial cells (Tepass et al., 1990). Several years later, it was found that mutations in a human homolog of the Drosophila melanogaster protein crumbs, denoted as CRB1 (Crumbs homolog 1), was involved in retinal dystrophies in humans (). The human CRB1 gene is mapped to chromosome 1q31.3, and contains 12 exons, has 12 identified transcript variants so far, three CRB family members, and over 210 kb genomic DNA ()1. Canonical CRB1 is, like its Drosophila homolog, a large transmembrane protein consisting of multiple epidermal growth factor (EGF) and laminin-globular like domains in its extracellular N-terminus (Figure 1A). The intracellular C-terminal domain contains a FERM and a conserved glutamic acid-arginine-leucine-isoleucine (ERLI) PDZ binding motives. An alternative transcript of CRB1, CRB1-B, was recently described and suggested to have significant extracellular domain overlap with canonical CRB1 while bearing unique 5′ and 3′ domains (Ray et al., 2020). In mammals, CRB1 is a member of the Crumbs family together with CRB2 and CRB3 (Figure 1A). CRB2 displays almost the same protein structure as CRB1, except a depletion of four EGF domains. CRB3A lacks the entire typical extracellular domain but contains the transmembrane domain juxtaposed to the intracellular part with the FERM-binding motif and a ERLI PDZ sequence. A second protein (isoform CRB3B) arises from the same CRB3 gene due to alternate splicing of the last exon, resulting in a different C-terminus with a cysteine-leucine-proline-isoleucine (CLPI) amino acid sequence, and thus lacks the PDZ domain (; Margolis, 2018). Interestingly, the CRB3B isoform is found in mammals, but not in zebrafish or Drosophila (). Further details about CRB isoform details can be found in Quinn et al. (2017).
FIGURE 1
CRB Localization in the Retina
In mammalian tissue CRB1 and CRB2 are predominantly expressed in the retina, however, CRB2 expression is also found in other tissues such as in kidney podocytes, in the subventricular zone of the brain, and in the spinal cord (; Slavotinek et al., 2015; ; Tait et al., 2020). Within the retina, CRB proteins are localized at the subapical region (SAR) above the adherence junctions between photoreceptor and Müller glial cells (MGCs), multiple photoreceptor cells (PRCs), or between multiple MGCs (Pellikka et al., 2002; van de Pavert et al., 2004). In addition, unlike CRB1, CRB2 also localizes in the retinal pigment epithelium (RPE). Defining the subcellular localization is essential to understand the function of CRB proteins (Figure 1B). Mouse studies have shown that full length Crb1 protein is exclusively present in MGC at the SAR while Crb2 and Crb3 are present in both PRC and MGC (van Rossum et al., 2006). Serial tangential cryosectioning of the retina followed by western blotting displays Crb1-B transcript expression in outer segments of PRCs in mice (Ray et al., 2020). The first ultrastructural data in postmortem human retina revealed a different localization, where CRB2 is located in MGC at the SAR and at vesicles in photoreceptor inner segments, whereas CRB1 is located in both MGC and PRC at the SAR (Pellissier et al., 2014b, 2015). CRB3A was found in microvilli of MGC at the SAR and in inner segments of PRC (Pellissier et al., 2014b, 2015). However, recently, it was shown that CRB1 and CRB2 are located in MGC and PRC at the SAR in the second trimester of human fetal retina and in human iPSC-derived retinal organoids, whereas in the first trimester only CRB2 was detected (Quinn et al., 2019a). Single-cell RNA sequencing data of human fetal retina and RPE confirms that CRB1 is present in retinal progenitor cells and MGC but not in RPE, which is in accordance with mouse versus primate localization studies (Hu et al., 2019). In addition, similar localization for CRB1, CRB2, and CRB3A were detected in rhesus and cynomolgus macaques (Quinn et al., 2019b). CRB3A was also detected in the inner retina and RPE of both rhesus and cynomolgus macaques (Quinn et al., 2019b). These recent data suggest that, in humans, CRB2 might also be present at the SAR membranes in PRC rather than only in vesicles of photoreceptor inner segments. The discrepancies in CRB2 pattern at the SAR observed in postmortem human retinas versus monkeys, fetal and retinal organoids could be explained by the age of the donors studied, the quality of the samples (processed within 48 h after death), or by technical issues. We could speculate that CRB2 may have a different location in human aged retinas, CRB2 at the SAR might have not been detected, or CRB2 might have been endocytosed from the PRC plasma membrane following donor death. Additional experiments with fresh human retina defining the subcellular localization of CRB1 and CRB2 could potentially resolve these differences. In summary, according to all these evidences, we hypothesize that in primates, including humans, CRB1 and CRB2 are located in both cell types at the SAR. Therefore, both MGC and PRC should be targeted to prevent retinal degeneration in RP patients.
Recently, Crb trafficking to the correct apical location in Drosophila epithelium has been further investigated (Figure 1C; Li et al., 2007; Pocha et al., 2011; Kraut and Knust, 2019; ). Crb is correctly localized by Rab11-containing endosomes using motor-driven transport along polarized microtubules and F-actin filaments. Interestingly, upon loss of microtubule minus-end director protein dynein, Rab11 endosomes containing Crb are transported basally rather than apically (). Once Crb is successfully addressed to the apical membrane, it is delivered at the correct localization on the plasma membrane using exocyst-mediated delivery. Mutant clones for sec15 or sec5, subunits of the exocyst, strongly disrupts the apical localization of Crb, confirming the essential requirement of the exocyst in delivery of Crb to the apical membrane (). In cultured mammalian cells, the exocyst associates with adherens junctions and PAR3 (; Polgar and Fogelgren, 2018). Because of the known interaction between CRB, PAR6, and PAR3 in mammals, mentioned below, this trafficking model might be conserved among species. Further investigations are required to test whether the mechanisms of epithelial polarization are conserved in humans.
CRB Protein Function in Mammalian Tissues
Various research studies have shown that CRB1 and CRB2 are apical polarity factors, and apical-basal cell polarity is essential for the formation and function of epithelial tissues (). More research is required to define the function of the recently described CRB1-B isoform because of its distinct 5′ and 3′ domain. Below, we will describe the canonical function of CRB in maintaining cell adhesion and morphogenesis, and its role in cell division and development.
Maintaining Cell Adhesion and Morphogenesis
The prototypic ERLI sequence of CRB proteins is important for interaction with key adaptor proteins. The core CRB complex is formed by interaction of CRB and protein associated with Lin Seven 1 (PALS1), also known as membrane-associated guanylate kinase p55 subfamily member 5 (MPP5), where PALS1 binds to the conserved C-terminal PDZ domain of CRB (Roh et al., 2002; van de Pavert et al., 2004; Margolis, 2018). Ablation of Mpp5 in mouse RPE causes early onset retinal degeneration, whereas ablation of Mpp5 in the neural retina does not, suggesting an essential role of PALS1 at the tight junctions of RPE but not in the neural retina (Park et al., 2011). The core CRB complex is evolutionary conserved and regulates apical-basal polarity and maintains cell adhesion (). Pals1 can interact with Mpp3 and Mpp4 at the SAR in the mouse retina (Figure 2A; Kantardzhieva et al., 2005, 2006; ). Mpp3 conditional knockout (cKO) mice with Mpp3 specifically ablated in the retina showed disrupted localization and reduced levels of Pals1, indicating that Mpp3 is essential to maintain levels of Pals1 at the SAR near the outer limiting membrane ().
FIGURE 2
Additionally, binding of PALS1 and CRB can lead to the recruitment of PATJ or multiple PDZ domain protein 1 (MUPP1) to the apical membrane (Figure 2B; Roh et al., 2002). PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells (Michel et al., 2005). In some cells, both MUPP1 and PATJ complexes co-exist, and MUPP1 regulates the cellular levels of the PALS1/PATJ polarity complex (). Co-immunoprecipitation studies showed that Mupp1 interacts in the mouse retina with Crb1 and Pals1, but not or less with Patj (van de Pavert et al., 2004). PATJ preferentially binds with PALS1 and partitioning defective-6 homolog (PAR6; Figure 2B; ; ). PAR6 is a key adaptor protein that interact with the ERLI PDZ domain of CRB in mammalian cells (Hurd et al., 2003; Lemmers et al., 2004). PAR6 leads to recruitment of PAR3, atypical protein kinase C (αPKC) and cell division control 42 (CDC42), known as the PAR complex (Joberty et al., 2000; Lin et al., 2000; Suzuki et al., 2001; Yamanaka et al., 2001; Hurd et al., 2003; Whitney et al., 2016; Pichaud, 2018). This PAR6-CDC42 complex is required for the apical-basal polarity and cell adhesion (Yamanaka et al., 2001).
Alternatively, the importance of a CRB-PALS1-EPB4.1L5 complex in mammals has been described (Laprise et al., 2006, 2009; ). Co-expression and co-localization studies suggested that in several epithelial derived tissues Epb4.1l5 interact with at least one Crumbs homolog and with PALS1 (Figure 2C). In addition, in the adult retina, Epb4.1l5 showed substantial overlap at the outer limiting membrane (OLM) with CRB1 and PALS1 (). Overexpression of Epb4.1l5 in polarized MDCK cells affects tightness of cell junctions and results in disorganization of the tight junction markers ZO-1 and PATJ (). However, another group discovered, unlike in the zebrafish and Drosophila orthologs, that mouse Crumbs proteins are localized normally in absence of Epb4.1l5 (Lee et al., 2007). Additional research should be performed to define its precise molecular mechanism in mammalian cells.
The alternative isoform CRB3B contains a distinct carboxy terminal motif namely the CLPI motif, suggesting different binding partners in epithelial cells. CRB3 is widely expressed in epithelial cells. A Crb3 KO mouse demonstrates extensive defects in epithelial morphogenesis, the mice die shortly after birth with cystic kidneys and lung proteinaceous debris throughout the lungs (Whiteman et al., 2014). Interestingly, these defects are also seen in Ezrin knockout mice, which is in line with the detected interaction between CRB3B and Ezrin in mice and mammalian cells (Figure 2D; Whiteman et al., 2014; Tilston-Lünel et al., 2016). This indicates that CRB3B is also crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying cytoskeleton (Whiteman et al., 2014; ; Tilston-Lünel et al., 2016). Therefore, the roles of the two CRB3 variants in the mouse lungs remain to be determined.
Drosophila Crb has also been found to inhibit the positive-feedback loop of phosphoinositide 3-kinase (PI-3K) and Rac1, thereby repressing the activation of Rac1 as well as PI-3K and maintaining proper apical domain and epithelial tissue integrity (Figure 2E; ). This process could potentially be conserved in different species, however, more research in mammalian cells is required to support this hypothesis.
CRB Function in Cell Proliferation
In MGCs of Drosophila and Xenopus, yes-associated protein 1 (YAP) an important role in damaged retina (Hamon et al., 2017, ; Rueda et al., 2019). YAP is a core member of the Hippo pathway, which regulates several biological processes including cell proliferation, and survival (Yu et al., 2015). The intracellular domain of Drosophila Crb interacts with Expanded and thereby regulates the activity of Hippo pathway kinases (Yu and Guan, 2013; Yu et al., 2015). In mammalian lung epithelial and breast cancer cells, CRB3 expression also correlates with the Hippo pathway (Szymaniak et al., 2015; Mao et al., 2017). More specifically, CRB3 affects the Hippo pathway by interacting with Kibra and/or FRMD6 (FRMD6 is the homolog of Drosophila Expanded; Figure 2F). With low CRB3 expression levels, the Hippo-pathway is inactivated, YAP is not phosphorylated and can move to the nucleus where YAP target genes are expressed leading to increased cell proliferation and decreased apoptosis (Mao et al., 2017). Also in Crb1rd8 versus wild-type mouse retina several Hippo signaling related genes were differentially expressed (Hamon et al., 2017). In addition, CRB1 and CRB2 deletion also lead to YAP signaling dysregulation in developing murine retinas (Pellissier et al., 2013). These data suggest an essential role of the Hippo pathway in the control of cell proliferation.
CRB1 and CRB2 in Retinal Diseases
Mutations in the CRB1 gene are associated with a wide spectrum of retinal dystrophies, such as retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA). RP is a clinically and genetically heterogeneous disease affecting more than 1.5 million people worldwide, where patients typically experience night blindness followed by progressive visual field loss ultimately leading to complete loss of vision in early or middle-life (Talib et al., 2017; Verbakel et al., 2018). The age at symptom onset for RP patients ranged from 0 to 47 years, with a median onset of 4 years (Talib et al., 2017). Approximately 3–9% of non-syndromic cases of autosomal recessive RP are caused by a mutation in the CRB1 gene (Vallespin et al., 2007; ; ). LCA is a more severe retinal dystrophy, causing serious visual impairment or blindness in newborns (). Mutations in the CRB1 gene account for approximately 7–17% of all LCA cases (; Vallespin et al., 2007; ; ). There are more than 200 different mutations described along the CRB1 gene resulting in retinal dystrophies without a clear genotype-phenotype correlation with RP or LCA, CRB1 patients may display unique clinical features such as pigmented paravenous chorioretinal atrophy, macular atrophy alone, retinal degeneration associated with Coats-like exudative vasculopathy, para-arteriolar preservation of the RPE, or nanophthalmia (Henderson et al., 2011; ; ). The clinical variability of disease onset and severity, even within a patient cohort with the same homozygous mutations, supports the hypothesis that the phenotype of patients with CRB1 mutations is modulated by other factors (Mathijssen et al., 2017). So far, no treatment options exist for patients with mutations in the CRB1 gene. To study CRB1-related retinal dystrophies for treatment options, several models have been used. Below, CRB1-related human- and animal-derived retinal models are described.
Until recently, no RP patients were described with mutations in the CRB2 gene. However, discovered, using whole exome sequencing (WES), a homozygous CRB2 p.R1249G mutation in a consanguineous Chinese family presenting RP. This mutation disturbs the stability of CRB2 protein and thereby induces RPE degeneration, impairs RPE phagocytosis, and accelerates RPE apoptosis. However, only a limited number of patients with this mutation are described, identification of CRB2 mutations in more RP patients is warranted to better support its pathogenicity.
Human-Derived Retinal Models
The use of human-induced pluripotent stem cell (hiPSC) models for research is an emerging strategy to explore patient phenotypes in vitro. These techniques allow access to previously limited or inaccessible material and have been explored in many ophthalmic laboratories worldwide. A commonly used method is the differentiation of hiPSC into retinal organoids. Since the first one, numerous groups have adapted or created their own method to more efficiently generate well laminated retinal organoids (Meyer et al., 2011; Nakano et al., 2012; Zhong et al., 2014; Luo et al., 2018; Ovando-Roche et al., 2018). Nevertheless, a wide variability in differentiation efficiency across hiPSC lines is often reported (; ; Mellough et al., 2019; ). have shown that the ability of three different hiPSC lines to differentiate into retinal organoids in response to IGF1 or BMP4 activation was line- and method-dependent. differentiated five hiPSC lines with a variability in efficiency, but by 5 months of differentiation all the retinal organoids were able to generate light responses and contained a well-formed ONL with PRCs containing inner segments, cilia, and outer-like segments. Attempts have been made to decrease this wide variability, Luo et al. (2018) described that the use of a Wnt signaling pathway antagonist, Dickkopf-related protein 1 (DKK-1), efficiently generated retinal organoids in all six hiPSC lines. Bulk RNA-sequencing profiling of retinal organoids demonstrated that the retinal differentiation in vitro recapitulated the in vivo retinogenesis in temporal expression of cell differentiation markers, retinal disease genes, and mRNA alternative splicing (Kim et al., 2019). These results make the retinal organoids, despite their high variability, of great interest in a wide range of applications including drug discovery, investigating the mechanism of retinal degeneration, developing cell-based therapeutic strategies and many more.
Defining the localization and onset of expression of the CRB complex members has been achieved in healthy hiPSC-derived retinal organoids. Several members of the CRB complex, CRB2, PALS1, PATJ, and MUPP1, were detected at the outer limiting membrane as early as differentiation day 28 (DD28), typical and clear puncta-like staining patterns for CRB1 were found only after DD120. All CRB complex members together with adherence junction markers, p120-catenin and N-cadherin, were still detectable in DD180 retinal organoids (Quinn et al., 2019a). The onset of CRB1 and CRB2 protein expression recapitulates those observed in the human fetal retina, with a clear onset of CRB2 expression before CRB1 expression (Quinn et al., 2019a).
Three CRB1 patient hiPSC lines containing a homozygous missense mutation (c.3122T > C), or heterozygous missense mutations (c.2983G > T and c.1892A > G, or c.2843G > A and c.3122T > C) were successfully differentiated into retinal organoids and analyzed at DD180. Here, all three retinal layers were developed: retinal ganglion cell layer marked by Tuj1 positive dendrites, neuroblast layer marked by SOX9 positive retinal progenitor cells, and an outer nuclear layer marked by recoverin positive PRCs. However, frequently, there were ectopic recoverin positive cells found above the outer limiting membrane and all missense CRB1 organoid lines developed small but frequent disruptions of localization of CRB complex members at the OLM that were not found in control lines (Quinn et al., 2019a). Data from these CRB1 patient hiPSC retinal organoids suggest a retinal degeneration phenotype similar to that previously found in mice lacking CRB1, mice expressing the C249W CRB1 variant, or mouse retina lacking CRB2 (van de Pavert et al., 2004, 2007a; ). Another study shortly describes the successful differentiation of hiPSC carrying a compound heterozygous mutation in the CRB1 gene (c.1892A > G and c.2548G > A) to retinal organoids. All three germ layers and expressed markers of retinal progenitor cells, including N-cadherin, rhodopsin, and PAX6, after 35 days of differentiation were present, but no phenotype was described in this paper (Zhang et al., 2018). To our knowledge, only these two papers have reported the generation of CRB1 patient hiPSC-derived retinal organoids. The reproducible phenotype observed in these three CRB1 patient lines (Quinn et al., 2019a) provides a good model for assessing potential gene therapy approaches.
Another frequently used method in the ophthalmic field is the differentiation of hiPSC into RPE monolayers. Efficient protocols for differentiating hiPSC into RPE monolayers using a mixture of growth factors have been established (Zahabi et al., 2012; ; Shutova et al., 2016). However, attempts are currently made to use non-biological products, such as small molecules, that would limit the risks of infection or immune rejection when transplanted. Maruotti et al. (2015) developed a protocol which uses chemotin (CTM) in combination with a previously known neural inducer nicotinamide (NIC) to efficiently differentiate hiPSC into RPE monolayers. In three independent hiPSC lines, RPE differentiation was efficient, and key RPE markers such as microphthalmia-associated transcription factor (MITF), PMEL17, and tight junction protein zonula occludens 1 (ZO-1) were strongly expressed. When left longer in culture to mature, bestrophin 1 (BEST1) and RPE-specific protein of 65 kDa (RPE65) were also strongly expressed (Maruotti et al., 2015). Using a slightly adapted protocol, Smith et al. (2019) differentiated six more hiPSC lines into hiPSC-RPE monolayers, all six also expressed the key RPE markers ZO-1, BEST-1, and MITF. Another study revealed by RNA sequencing data that hiPSC-RPE grouped with fetal RPE samples, indicating that their gene expression was highly correlated and similar (Smith et al., 2019). In addition, Zahabi et al. (2012) provided proof-of-concept that multiple retinal-disease specific hiPSC lines, including two RP lines, can be differentiated into RPE monolayers. Altogether, this data illustrates the potential of hiPSC-RPE as a model system for retinal diseases with mutations in the RPE. Related to CRB, recent studies have shown that CRB2 but not CRB1 is expressed in the human RPE during the differentiation into retinal organoids (Quinn et al., 2019a). In addition, there are RP patients described with specific CRB2 variations expressed in RPE cells (). Therefore, the method of generating hiPSC-RPE could be used to explore treatment possibilities for patients with specific variations in CRB2.
CRB1-Related Animal Retinal Degeneration Models
Numerous research groups focus on animal models to gain, understand, and develop gene therapy strategies that potentially can be used to treat retinal degeneration of RP and LCA patients. Over the years there are multiple animal models developed mimicking the CRB1-related phenotype in patients. These models vary from mild to more severe, early- to late-onset, and MGC or photoreceptor specific phenotypes. Double retinal knock-outs of CRB1 and CRB2, have helped to understand the contribution of the two CRB proteins to the retinal disease etiology, and explain the relatively mild phenotype observed in Crb1 variant mouse models (Mehalow et al., 2003; van de Pavert et al., 2004, 2007a,b).
LCA-Like Mouse Models
Four mouse models showing a CRB1 LCA-like phenotype have been reported: Crb1KOCrb2ΔRPC where both Crb1 and Crb2 are ablated in retinal progenitor cells (Pellissier et al., 2013), secondly the Crb1KOCrb2ΔimPRC where Crb1 is ablated in MGC and Crb2 is ablated in immature PRCs with remaining Crb2 levels in MGC and progenitor cells (Quinn et al., 2018), thirdly the Crb1KO/WTCrb2ΔRPC mouse model with reduced levels of Crb1 in MGC and ablation of Crb2 in retinal progenitor cells (Pellissier et al., 2013), and finally Crb1KOCrb2ΔMGC in which both Crb1 and Crb2 are ablated in MGC (Quinn et al., 2019b). All four models exhibit vision loss indicated by a reduced electroretinography (ERG) response. In addition, retinal degeneration was observed by outer limiting membrane disruptions, abnormal retinal lamination, intermingling of nuclei of the ONL and INL, and ectopic localization of retinal cells. These mouse models show an early onset phenotype with distinct severity indicated by the order mentioned above. In short, in the most severe mouse model, Crb1KOCrb2ΔRPC, the phenotype onset was found as early as embryonal day 13 (E13) which was observed throughout the retina (Pellissier et al., 2013). Retinal degeneration in Crb1KOCrb2ΔimPRC was detected at E15 in the whole retina, but in adult mice the superior retina was more affected than the inferior retina (Quinn et al., 2018). Also in Crb1KO/WTCrb2ΔRPC mice retinal degeneration was detected at E15, but was mostly affecting the peripheral retina (Pellissier et al., 2013). Finally, the Crb1KOCrb2ΔMGC mice showed the first signs of degeneration at E17, where mostly the peripheral retina was affected. More subtle differences between these models are described and summarized before (Quinn et al., 2019b). These data show that all four Crb1 mouse models mimic the LCA phenotype in patients and could therefore be used for future therapy development.
RP-Like Mouse Models
Twelve CRB1 RP-like mouse models have been described so far, including (1) Crb1KO/C249W with a missense variation in the Crb1 gene (van de Pavert et al., 2007a), (2) Crb2ΔMGC where only Crb2 is ablated in MGC (), (3) the Crb1rd8 mice with a naturally occurring nonsense mutation in the Crb1 gene (Mehalow et al., 2003), (4) the Crb1KO where the full length Crb1 protein is ablated from retinal radial glial progenitor cells, MGCs, and the rest of the body (van de Pavert et al., 2004), (5) the Crb1del–B were Crb1-B is abolished from photoreceptor and MGCs (Ray et al., 2020), (6) the Crb1null where a deletion of alternate exon 5a up to intron 7 disrupts all Crb1 isoforms (Ray et al., 2020), (7) Crb2Δrods where Crb2 is ablated only in developed rod PRCs (), (8) Crb1KOCrb2Δrods where Crb1 is ablated in MGC and Crb2 in rod PRCs (), (9) Crb1KOCrb2low–imPRC with absence of Crb1 and reduced levels of Crb2 in immature photoreceptors (Quinn et al., 2018), (10) Crb1KOCrb2Δlow–RPC with absence of Crb1 and reduced levels of Crb2 in retinal progenitor cells (Pellissier et al., 2014b), (11) Crb2ΔRPC where Crb2 is ablated in retinal progenitor cells (), and finally, (12) Crb2ΔimPRC with Crb2 ablation in immature PRCs (). The different Cre mouse models show variations in mosaicism (mutant adjacent to wildtype cells), but eleven out of twelve Crb models show disruptions at the outer limiting membrane with rows or single photoreceptor nuclei protruding into the subretinal space or ingressing into the outer plexiform layer leading to a degenerative retinal phenotype (Table 1).
TABLE 1
| Crb1KO/C249W | Crb2ΔMGC | Crb1del–B | Crb1rd8 | Crb1KO | Crb1null | Crb2ΔRods | Crb1KOCrb2ΔRods | Crb1KOCrb2low–imPRC | Crb1KOCrb2low–RPC | Crb2ΔRPC | Crb2ΔimPRC | |
| Severity | + | + | − | ++ | ++ | ++ | ++ | +++ | +++ | +++ | ++++ | ++++ |
| CRB1-A ablation | MGC | − | − | Natural occurring mutation | MGC | MGC | − | MGC | MGC | MGC | − | − |
| CRB1-B ablation | − | − | PRC and MGC | Natural occurring mutation | − | PRC and MGC | − | − | − | − | − | − |
| CRB2 ablation | − | MGC | − | − | − | − | Rod PRC | Rod PRC | 50% in immature PRC | 50% in RPC | RPC | Immature PRC |
| Morphologic phenotype onset∗ | 8M | 1M | NA | 1M | P14 | 3M | 3M | 1M | P10 | P10 | E18.5 | E15.5 |
| ERG differences∗ | No | No | NA | No | No | NA | Yes (9 M) | Yes (3 M) | Yes (3 M) | Yes (3 M) | Yes (1 M) | Yes (1 M) |
| Affected areas | NA | Mainly periphery | NA | Inferior nasal quadrant | Inferior temporal quadrant | Inferior retina | Superior retina | Superior retina | Inferior retina | Throughout retina | Throughout retina | Throughout retina |
| OLM disruptions | Yes, sporadic | Yes | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| PRC nuclei protrusions | Yes, sporadic | Yes | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
Schematic overview retinitis pigmentosa (RP) mouse models.
MGC, Müller glial cells; OLM, outer limiting membrane; PRC, photoreceptor cell; RPC, retinal progenitor cell. ∗ The phenotype onset and ERG differences can be marginal on the indicated ages, the severity of the phenotype increases over time.
The Crb1rd8 mice have a naturally occurring single base deletion in exon 9 of the Crb1 gene causing a frame shift and premature stop codon, thereby truncating the transmembrane and cytoplasmic domain of Crb1. This results in a photoreceptor degeneration mainly observed in the inferior nasal quadrant of the eye, caused by retinal folds and pseudorosettes (Mehalow et al., 2003). In the Crb1KO mouse model the retinal lamination is predominantly maintained, and degeneration is found in the inferior temporal quadrant of the retina. Degeneration is indicated by single or groups of PRCs protruding into the subretinal space, rosette formation, and neovascularization. In 18M-old mice there was no loss of overall retinal function measured by electroretinography, suggesting that a major part of the retina was not affected by loss of Crb1 (van de Pavert et al., 2004, 2007b). Light exposure experiments reveal that light exposure doesn’t initiate but rather enhances the retinal degeneration (van de Pavert et al., 2007b). The mild phenotype observed in these Crb1 mouse models suggests that Crb2 protein may compensate the effect of CRB1 deletion.
In the Crb1del–B the alternative Crb1-B isoform is abolished from PRCs, while the Crb1null disrupts all potential Crb1 isoforms (Ray et al., 2020). The Crb1del–B mouse do not show significant disruptions in the OLM, while the disruptions in the Crb1null mice were comparable with Crb1rd8 (Ray et al., 2020). Although not mentioned by Ray et al. (2020), also the Crb1KO mice shows significant OLM disruptions strongly depending on genetic background as well as exposure to light (van de Pavert et al., 2004, 2007b). As the IrCaptureSeq suggested that Crb1-B is the most abundant transcript in mouse and human retina, a cross-breeding of Crb1null with Crb1del–B mice was performed to define the relevance of Crb1-B in the retina. This heterozygous Crb1null/del–B showed similar OLM disruptions with the homozygous Crb1null mouse (Ray et al., 2020). Similar OLM disruptions were thus found in Crb1rd8, Crb1null, Crb1null/del–B, and Crb1KO mice. It is essential to perform retinal function measurements on the mouse models affecting Crb1-B to understand its function and to compare it with previously described Crb1 mouse models. In addition, the most severe retinal phenotype so far derives from Crb1 mice with concomitant loss of Crb2. Both Crb2ΔRPC and Crb2ΔimPRC show an early onset retinal degeneration at embryonic day 18.5 (E18.5) and E15.5, respectively, the difference is caused by the distinct expression pattern and timing of the Cre recombinase and morphological phenotypes result in differences in the scotopic and photopic ERG conditions already at 1 M of age (, ). Interestingly, in contrast to Crb2ΔRPC mice, the lamination of Müller glial, ganglion and amacrine cells were also misplaced in Crb2ΔimPRC mice. These lamination defects were also observed in Crb1KO/WTCrb2ΔRPC mice (Pellissier et al., 2013).
Another mouse model, Crb2Δrods, show a mild and late onset phenotype limited to the superior retina (). In some 3 M and all 6 M old Crb2Δrods mice, disruptions of the outer limiting membrane, a thinned photoreceptor layer at the peripheral superior retina, and PRCs protruding into the subretinal space were observed. A reduction in ERG scotopic a-wave was observed in 9 M old mice, while no difference in optokinetic head tracking response (OKT) spatial frequency or contrast sensitivity was observed. Interestingly, while most PRCs were affected, all the remaining PRCs showed mature inner- and outer-segments. Remaining rods were functional and the cones showed normal morphology. This phenotype is enhanced by a concomitant loss of Crb1 in MGCs (). These mice, Crb1KOCrb2Δrods, show a similar but enhanced phenotype in comparison to Crb2Δrods mice. Here, a reduction in ERG scotopic a-wave was observed at 3 M and became more apparent at 6 M onward. In addition, a significant decrease in OKT contrast sensitivity was found from 3 M of age onward (). The difference in phenotype onset between all these mouse models might be explained by the onset of Cre expression during retinogenesis and by cell-type specific ablation; ablation of Crb2 from a later time point resulted in a milder phenotype. Interestingly, when Crb2 is ablated in MGCs, Crb2ΔMGC, a very mild morphological phenotype with no functional consequences measured using ERG was observed (), but ablation of both Crb1 and Crb2 from MGCs caused a severe LCA phenotype (Quinn et al., 2019b). This data suggests that Crb2 has a redundant function in MGCs, while in PRCs it is essential for proper retinal lamination and function.
Interestingly, the Crb1KO phenotype is located at the inferior temporal quadrant whereas the Crb2Δrods phenotype is mainly observed at the peripheral and central superior retina. These differences might be related to higher levels of Crb2 in the inferior retina while Crb1 is expressed at higher levels in the superior retina (Pellissier et al., 2014b). In addition, there might be modifying factors present which are enriched in either the superior or inferior retina causing the different phenotypes.
In addition to these mouse models, a rat with a spontaneous mutation in Crb1 exon 6 was discovered mimicking human macular telangiectasia type 2 (Zhao et al., 2015). The autosomal recessive indel mutation causes an early onset phenotype with a strongly reduced ERG response in 3-week old rats. These rats showed a focal loss of retinal lamination, OLM disruptions, and PRC, MGC, and RPE alterations (Zhao et al., 2015). Differences between this and the Crb1 mouse models could result from different types of mutations or from different genetic setups displayed by these different animal species.
Gene Augmentation Strategies for CRB1 Retinal Dystrophies
There is an emerging interest in gene augmentation strategies for retinal dystrophies. Recently, gene therapy became available for young RP and LCA patients with biallelic mutations in the RPE65 gene. Voretigene neparvovec-rzyl, or its commercial name: LUXTURNATM, uses the adeno-associated viral vector serotype 2 (AAV2) to deliver by subretinal injection a functional copy of the RPE65 gene into the RPE cells. RPE65 transgene expression results in the production and correct localization of RPE65 protein in RPE cells, thereby compensating for the loss of the protein and restoring the visual cycle in these patients (Maguire et al., 2019). Nowadays, there are numerous clinical studies ongoing which explore the use of AAV as a therapeutic vector for retinal diseases, such as RP, wet age-related macular dystrophy (AMD), LCA, and many more (Wang et al., 2019). However, so far, no treatment options are available for RP and LCA patients with mutations in the CRB1 gene. Below, we will describe novel therapeutic tools which could be promising for CRB1 retinal gene augmentation therapy in RP patients.
Currently, AAVs are the leading platform for gene delivery in the treatment of retinal dystrophies. AAVs are mainly investigated because of their low toxicity, their capability to transduce both dividing and non-dividing cells, they do not integrate into the host genome, and AAV capsid variants display distinct cell tropisms. A complete overview of basic AAV biology, AAV vectorology, and current therapeutic strategies and clinical progress was recently reviewed by Wang et al. (2019) and . The major disadvantage of AAVs is their limited package capacity, bigger gene expression cassettes than 4.5 kb are not able to fit within a single AAV. Because of this, the development of AAV-mediated CRB1 gene therapy is also challenging. Full-length cytomegalovirus (CMV) ubiquitous promoter and CRB1 cDNA exceeds the AAV package limitation. However, using an engineered minimal CMV promoter and codon optimized CRB1 cDNA allowed sufficient expression levels of full-length CRB1 protein in MGCs in mice (Pellissier et al., 2014a). Pre-clinical studies in mouse using this AAV-CMVmin-hCRB1 have shown that expression of CRB1 was deleterious in CRB1 mouse models but not in wild-type mice (Pellissier et al., 2015). In addition, there are more potential CRB1 transcript variants which could be targeted (Quinn et al., 2017). Interestingly, alternative strategies using the codon-optimized structural and functional family member CRB2 to rescue CRB1-related retinopathies restored retinal function and structure in Crb1 mouse models (Pellissier et al., 2015). In that study, improved photoreceptor layer morphology and ERG response was detected after CRB2 delivery. The combination of AAV9 with a full-length CMV promoter was used to target both photoreceptor and MGCs, whereas no rescue was observed when either photoreceptor or MGCs were targeted with CRB2 (Pellissier et al., 2015). In addition, several groups explored the possibility to overcome the limiting AAV package capacity by dual or triple-AAV approaches (Trapani et al., 2014; ; Maddalena et al., 2018) or by increasing the package capacity (). Yet, the studies performed so far have shown that the dual AAV approaches have lower expression levels compared to a single AAV vector (Trapani et al., 2014; ).
Several research groups focus on AAV-mediated gene delivery to define the tropism in hiPSC-derived retinal organoids and/or RPE. Out of four different AAV capsids packaged with the CAG promoter and GFP (AAV2, AAV2-7m8, AAV8, AAV9), showed with AAV2-7m8 infection at day 44 the most efficient transduction of the hiPSC-derived retinal and RPE organoids. Limited transduction of AAV9 was found (). Another study showed, using AAV-CMV-GFP constructs, a more efficient retinal organoid transduction at DD220 especially in MGC with AAV5 or ShH10Y445F capsids in comparison with AAV9 (Quinn et al., 2019a). Recently, Lane et al. (2020) demonstrate efficient PRCs transduction in DD140 retinal organoids using an AAV5 packaged with the CAG promoter. Moreover, AAV5-mediated gene augmentation with human RP2 was able to rescue the degeneration found in RP2 KO retinal organoids by preventing ONL thinning and restoring rhodopsin expression (Lane et al., 2020). Therefore, testing CRB1- or CRB2-expressing vectors in retinal organoids will aid in the discovery of CRB gene therapy treatment for CRB1-related retinal dystrophies in patients.
In addition, there are different non-viral mediated approaches for gene therapy. An example is the use of nanoparticles. Three representative nanoparticles, namely metal-based, polymer-based, and lipid-based nanoparticles, were recently reviewed describing their characteristics and recent application in ocular therapy (Wang et al., 2018). Shortly, the most extensive characterized nanoparticle is the polymer-based CK30-PEG nanoparticle, which contains plasmid DNA compacted with polyethylene glycol-substituted lysine 30-mers (; Han et al., 2012). Theoretically, these approaches have an unlimited gene packaging capacity, which display a big advantage for large genes such as CRB1. These nanoparticles are also able to infect RPE and PRC, and can drive gene expression on a comparable scale and longevity than AAVs in mice (; Han et al., 2012). CK30-PEG nanoparticles have a tolerable safety profile and is non-toxic in mouse and non-human primate eyes (; ). To our knowledge, there is no successful clinical study described using these nanoparticles so far. More research is required to define the clinical relevance of these nanoparticles in retinal gene therapy. Further pre-clinical research and clinical trials will aid in the discovery of new gene therapy approaches for CRB1-related retinal dystrophies in patients. A retrospective CRB1 natural history study (NHS) has been published (Talib et al., 2017), and their prospective NHS with strategies for evaluation of the efficacy of novel clinical therapeutic interventions in the treatment of CRB1-retinal dystrophies will be reported in the end of 2020. Recently, a French biotech company called HORAMA signed an exclusive license agreement with Leiden University Medical Center targeting CRB1 gene mutations to treat inherited retinal dystrophies. Based on current timelines, HORAMA expects initiating a Phase I/II clinical study with the drug candidate in 2023 (HORAMA, 2020).
Conclusion
In this review we have discussed (1) CRB protein function in mammalian cells, (2) recent advances and potential tools for CRB1 human and animal retinal degeneration models, and (3) described therapeutic tools which potentially could be used for retinal gene augmentation therapy for RP and LCA patients with mutations in the CRB1 gene.
Statements
Author contributions
NB, JW, and LP contributed to writing, reviewing, and editing the manuscript. NB wrote the original draft. All authors contributed to the article and approved the submitted version.
Funding
Funding was obtained from the Foundation Fighting Blindness (TA-GT-0715-0665-LUMC), The Netherlands Organisation for Health Research and Development (ZonMw grant 43200004), the Million Dollar Bike Ride Grant Program (MDBR-19-131-CRB1), and Stichting Blinden-Penning (Uitzicht 2014-1 to LP and JW). LP acknowledges the funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 851231).
Conflict of interest
The LUMC is the holder of patent number PCT/NL2014/050549, which describes the potential clinical use of CRB2; JW and LP are listed as co-inventors of this patent, and JW is an employee of the LUMC. The remaining author declares that the research was conducted without any commercial or financial relationship that could be construed as a potential conflict of interest.
Footnotes
1.^http://grch37.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000134376;r=1:197170592-197447585
References
1
AdachiM.HamazakiY.KobayashiY.ItohM.TsukitaS.FuruseM.et al (2009). Similar and distinct properties of MUPP1 and Patj, two homologous PDZ domain-containing tight-junction proteins.Mol. Cell. Biol.292372–2389. 10.1128/mcb.01505-08
2
Aguilar-AragonM.FletcherG.ThompsonB. J. (2020). The cytoskeletal motor proteins Dynein and MyoV direct apical transport of crumbs.Dev. Biol.459126–137. 10.1016/j.ydbio.2019.12.009
3
AhmedS. M.MacaraI. G. (2017). The Par3 polarity protein is an exocyst receptor essential for mammary cell survival.Nat. Commun.8:14867. 10.1038/ncomms14867
4
AkelleyR. A.ConleyS. M.MakkiaR.WatsonJ. N.HanZ.CooperM. J.et al (2018). DNA nanoparticles are safe and nontoxic in non-human primate eyes.Int. J. Nanomedicine131361–1379. 10.2147/IJN.S157000
5
AlvesC. H.BoonN.MulderA. A.KosterA. J.JostC. R.WijnholdsJ. (2019). CRB2 loss in rod photoreceptors is associated with progressive loss of retinal contrast sensitivity.Int. J. Mol. Sci.20:4069. 10.3390/ijms20174069
6
AlvesC. H.PellissierL. P.VosR. M.GarridoM. G.SothilingamV.SeideC.et al (2014). Targeted ablation of Crb2 in photoreceptor cells induces retinitis pigmentosa.Hum. Mol. Genet.233384–3401. 10.1093/hmg/ddu048
7
AlvesC. H.Sanz sanzA.ParkB.PellissierL. P.TanimotoN.BeckS. C.et al (2013). Loss of CRB2 in the mouse retina mimics human retinitis pigmentosa due to mutations in the CRB1 gene.Hum. Mol. Genet.2235–50. 10.1093/hmg/dds398
8
AssématE.CrostE.PonserreM.WijnholdsJ.Le BivicA.Massey-HarrocheD. (2013). The multi-PDZ domain protein-1 (MUPP-1) expression regulates cellular levels of the PALS-1/PATJ polarity complex.Exp. Cell Res.3192514–2525. 10.1016/j.yexcr.2013.07.011
9
BazellièresE.AksenovaV.Barthélémy-RequinM.Massey-HarrocheD.Le BivicA. (2018). Role of the Crumbs proteins in ciliogenesis, cell migration and actin organization.Semin. Cell Dev. Biol.8113–20. 10.1016/j.semcdb.2017.10.018
10
BuchholzD. E.PenningtonB. O.CrozeR. H.HinmanC. R.CoffeyP. J.CleggD. O. (2013). Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.Stem Cells Transl. Med.2384–393. 10.5966/sctm.2012-0163
11
BuckT. M.WijnholdsJ. (2020). Recombinant adeno-associated viral vectors (rAAV)-vector elements in ocular gene therapy clinical trials and transgene expression and bioactivity assays.Int. J. Mol. Sci.21:4197. 10.3390/ijms21124197
12
BujakowskaK.AudoI.Mohand-SäidS.LancelotM. E.AntonioA.GermainA.et al (2012). CRB1 mutations in inherited retinal dystrophies.Hum. Mutat.33306–315. 10.1002/humu.21653
13
BulgakovaN. A.KnustE. (2009). The crumbs complex: from epithelial-cell polarity to retinal degeneration.J. Cell Sci.1222587–2596. 10.1242/jcs.023648
14
CaiX.NashZ.ConleyS. M.FlieslerS. J.CooperM. J.NaashM. I. (2009). A partial structural and functional rescue of a retinitis pigmentosa model with compacted DNA nanoparticles.PLoS One4:e0005290. 10.1371/journal.pone.0005290
15
CapowskiE. E.SamimiK.MayerlS. J.PhillipsM. J.PinillaI.HowdenS. E.et al (2019). Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines.Development146:dev171686. 10.1242/dev.171686
16
CarvalhoL. S.TurunenH. T.WassmerS. J.Luna-VelezM. V.XiaoR.BennettJ.et al (2017). Evaluating efficiencies of dual AAV approaches for retinal targeting.Front. Neurosci.11:503. 10.3389/fnins.2017.00503
17
ChartierF. J. M.HardyÉJ. L.LapriseP. (2011). Crumbs controls epithelial integrity by inhibiting Rac1 and PI3K.J. Cell Sci.1243393–3398. 10.1242/jcs.092601
18
ChenX.JiangC.YangD.SunR.WangM.SunH.et al (2019). CRB2 mutation causes autosomal recessive retinitis pigmentosa.Exp. Eye Res.180164–173. 10.1016/j.exer.2018.12.018
19
ChichagovaV.HilgenG.GhareebA.GeorgiouM.CarterM.SernagorE.et al (2020). Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent.Stem Cells38195–201. 10.1002/stem.3116
20
CoraV.HaderspeckJ.AntkowiakL.MattheusU.NeckelP. H.MackA. F.et al (2019). A cleared view on retinal organoids.Cells8:391. 10.3390/cells8050391
21
CortonM.TatuS. D.Avila-FernandezA.VallespínE.TapiasI.CantalapiedraD.et al (2013). High frequency of CRB1 mutations as cause of early-onset retinal dystrophies in the Spanish population.Orphanet J. Rare Dis.8:20. 10.1186/1750-1172-8-20
22
Den HollanderA. I.DavisJ.Van Der Velde-VisserS. D.ZonneveldM. N.PierrottetC. O.KoenekoopR. K.et al (2004). CRB1 mutation spectrum in inherited retinal dystrophies.Hum. Mutat.24355–369. 10.1002/humu.20093
23
Den HollanderA. I.Ten BrinkJ. B.De KokY. J. M.Van SoestS.Van Den BornL. I.Van DrielM. A.et al (1999). Mutations in a human homologue of Drosophila crumbs cause retinitis pigmentosa (RP12).Nat. Genet.23217–221. 10.1038/13848
24
DingX.GradinaruV. (2020). “Structure-guided rational design of adeno-associated viral capsids with expanded sizes,” in Proceedings of the 23rd Annual Meeting of the American Society for Gene and Cell Therapy, Milwaukee, WI.
25
DingX. Q.QuiambaoA. B.FitzgeraldJ. B.CooperM. J.ConleyS. M.NaashM. I. (2009). Ocular delivery of compacted DNA-nanoparticles does not elicit toxicity in the mouse retina.PLoS One4:e0007410. 10.1371/journal.pone.0007410
26
DudokJ. J.MurtazaM.AlvesH. C.RashbassP.WijnholdsJ. (2016). Crumbs 2 prevents cortical abnormalities in mouse dorsal telencephalon.Neurosci. Res.10812–23. 10.1016/j.neures.2016.01.001
27
DudokJ. J.SanzA. S.LundvigD. M. S.SothilingamV.GarridoM. G.KloosterJ.et al (2013). MPP3 regulates levels of PALS1 and adhesion between photoreceptors and Müller cells.Glia611629–1644. 10.1002/glia.22545
28
EbarasiL.AshrafS.BierzynskaA.GeeH. Y.McCarthyH. J.LovricS.et al (2015). Defects of CRB2 cause steroid-resistant nephrotic syndrome.Am. J. Hum. Genet.96153–161. 10.1016/j.ajhg.2014.11.014
29
FanS.FoggV.WangQ.ChenX. W.LiuC. J.MargolisB. (2007). A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin β interactions.J. Cell Biol.178387–398. 10.1083/jcb.200609096
30
FinkT. L.KlepcykP. J.OetteS. M.GedeonC. R.HyattS. L.KowalczykT. H.et al (2006). Plasmid size up to 20 kbp does not limit effective in vivo lung gene transfer using compacted DNA nanoparticles.Gene Ther.131048–1051. 10.1038/sj.gt.3302761
31
GaoY.LuiW. Y.LeeW. M.ChengC. Y. (2016). Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells.Sci. Rep.61–20. 10.1038/srep28589
32
Garita-HernandezM.RoutetF.GuibbalL.KhabouH.ToualbiL.RianchoL.et al (2020). AAV-mediated gene delivery to 3D retinal organoids derived from human induced pluripotent stem cells.Int. J. Mol. Sci.211–16. 10.3390/ijms21030994
33
GosensI.SessaA.den HollanderA. I.LetteboerS. J. F.BelloniV.ArendsM. L.et al (2007). FERM protein EPB41L5 is a novel member of the mammalian CRB-MPP5 polarity complex.Exp. Cell Res.3133959–3970. 10.1016/j.yexcr.2007.08.025
34
HallamD.HilgenG.DorgauB.ZhuL.YuM.BojicS.et al (2018). Human-induced pluripotent stem cells generate light responsive retinal organoids with variable and nutrient-dependent efficiency.Stem Cells361535–1551. 10.1002/stem.2883
35
HamonA.García-GarcíaD.AilD.BitardJ.ChesneauA.DalkaraD.et al (2019). Linking YAP to müller glia quiescence exit in the degenerative retina.Cell Rep.271712.e6–1725.e6. 10.1016/j.celrep.2019.04.045
36
HamonA.MassonC.BitardJ.GieserL.RogerJ. E.PerronM. (2017). Retinal degeneration triggers the activation of YAP/TEAD in reactive Müller cells.Retin. Cell Biol.581941–1953. 10.1167/iovs.16-21366
37
HanZ.ConleyS. M.MakkiaR.GuoJ.CooperM. J.NaashM. I. (2012). Comparative analysis of DNA nanoparticles and AAVs for ocular gene delivery.PLoS One7:e0052189. 10.1371/journal.pone.0052189
38
HendersonR. H.MackayD. S.LiZ.MoradiP.SergouniotisP.Russell-EggittI.et al (2011). Phenotypic variability in patients with retinal dystrophies due to mutations in CRB1.Br. J. Ophthalmol.95811–817. 10.1136/bjo.2010.186882
39
HORAMA (2020). HORAMA Signs Exclusive License Agreement with Leiden University Medical Center Targeting CRB1 Gene Mutations to Treat Inherited Retinal Dystrophies. Available online at: https://www.horama.fr/horama-signs-exclusive-license-agreement-with-leiden-university-medical-center-targeting-crb1-gene-mutations-to-treat-inherited-retinal-dystrophies/(accessed July 5, 2020).
40
HuY.WangX.HuB.MaoY.ChenY.YanL.et al (2019). Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis.PLoS Biol.17:e3000365. 10.1371/journal.pbio.3000365
41
HurdT. W.GaoL.RohM. H.MacaraI. G.MargolisB. (2003). Direct interaction of two polarity complexes implicated in epthelial tight junction assembly.Nat. Cell Biol.5137–142. 10.1038/ncb923
42
JobertyG.PetersenC.GaoL.MacaraI. G. (2000). The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42.Nat. Cell Biol.2531–539. 10.1038/35019573
43
KantardzhievaA.AlexeevaS.VersteegI.WijnholdsJ. (2006). MPP3 is recruited to the MPP5 protein scaffold at the retinal outer limiting membrane.FEBS J.2731152–1165. 10.1111/j.1742-4658.2006.05140.x
44
KantardzhievaA.GosensI.AlexeevaS.PunteI. M.VersteegI.KriegerE.et al (2005). MPP5 recruits MPP4 to the CRB1 complex in photoreceptors.Investig. Ophthalmol. Vis. Sci.462192–2201. 10.1167/iovs.04-1417
45
KimS.LoweA.DharmatR.LeeS.OwenL. A.WangJ.et al (2019). Generation, transcriptome profiling, and functional validation of cone-rich human retinal organoids.Proc. Natl. Acad. Sci. U.S.A.16610824–10833. 10.1073/pnas.1901572116
46
KrautR. S.KnustE. (2019). Changes in endolysosomal organization define a pre-degenerative state in the crumbs mutant Drosophila retina.PLoS One14:e0220220. 10.1371/journal.pone.0220220
47
LaneA.JovanovicK.ShortallC.OttavianiD.PanesA. B.SchwarzN.et al (2020). Modeling and rescue of RP2 retinitis pigmentosa using iPSC-derived retinal organoids.Stem Cell Rep.151–13. 10.1016/j.stemcr.2020.05.007
48
LapriseP.BeronjaS.Silva-gagliardiN. F.PellikkaM.JensenM.McgladeC. J.et al (2006). The FERM protein yurt is a negative regulatory component of the crumbs complex that controls epithelial polarity and apical membrane size.Dev. Cell11363–374. 10.1016/j.devcel.2006.06.001
49
LapriseP.LauK. M.HarrisK. P.Silva-GagliardiN. F.PaulS. M.BeronjaS.et al (2009). Yurt, Coracle, Neurexin IV and the Na+, K+-ATPase form a novel group of epithelial polarity proteins.Nature4591141–1145. 10.1038/nature08067
50
LeeJ. D.Silva-GagliardiN. F.TepassU.McGladeC. J.AndersonK. V. (2007). The FERM protein Epb4.1I5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo.Development1342007–2016. 10.1242/dev.000885
51
LemmersC.MichelD.Lane-GuermonprezL.DelgrossiM.-H.MédinaE.ArsantoJ. P.et al (2004). CRB3 binds directly to Par6 and regulates the morphogenesis of the tight junctions in mammalian epithelial cells.Mol. Biol. Cell151324–1333. 10.1091/mbc.E03
52
LiB. X.SatohA. K.ReadyD. F. (2007). Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors.J. Cell Biol.177659–669. 10.1083/jcb.200610157
53
LinD.EdwardsA. S.FawcettJ. P.MbamaluG.ScottJ. D.PawsonT. (2000). A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity.Nat. Cell Biol.2540–547. 10.1038/35019582
54
LuoZ.ZhongX.LiK.XieB.LiuY.YeM.et al (2018). An optimized system for effective derivation of three-dimensional retinal tissue via wnt signaling regulation.Stem Cells361709–1722. 10.1002/stem.2890
55
MaddalenaA.TornabeneP.TiberiP.MinopoliR.ManfrediA.MutarelliM.et al (2018). Triple vectors expand AAV transfer capacity in the retina.Mol. Ther.26524–541. 10.1016/j.ymthe.2017.11.019
56
MaguireA. M.RussellS.WellmanJ. A.ChungD. C.YuZ. F.TillmanA.et al (2019). Efficacy, safety, and durability of voretigene Neparvovec-rzyl in RPE65 mutation–associated inherited retinal dystrophy: results of phase 1 and 3 trials.Ophthalmology1261273–1285. 10.1016/j.ophtha.2019.06.017
57
MaoX.LiP.WangY.LiangZ.LiuJ.LiJ.et al (2017). CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells.Cell Death Dis.8:e2546. 10.1038/cddis.2016.478
58
MargolisB. (2018). The Crumbs3 polarity protein.Cold Spring Harb. Perspect. Biol.101–9. 10.1101/cshperspect.a027961
59
MaruottiJ.SripathiS. R.BhartiK.FullerJ.WahlinK. J.RanganathanV.et al (2015). Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells.Proc. Natl. Acad. Sci. U.S.A.11210950–10955. 10.1073/pnas.1422818112
60
MathijssenI. B.FlorijnR. J.Van Den BornL. I.Zekveld-VroonR. C.Ten BrinkJ. B.PlompA. S.et al (2017). Long-term follow-up of patients with retinitis pigmentosa type 12 caused by CRB1 mutations: a severe phenotype with considerable interindividual variability.Retina37161–172. 10.1097/IAE.0000000000001127
61
MehalowA. K.KameyaS.SmithR. S.HawesN. L.DenegreJ. M.YoungJ. A.et al (2003). CRB1 is essential for external limiting membrane integrity and photoreceptor morphogenesis in the mammalian retina.Hum. Mol. Genet.122179–2189. 10.1093/hmg/ddg232
62
MelloughC. B.CollinJ.QueenR.HilgenG.DorgauB.ZertiD.et al (2019). Systematic comparison of retinal organoid differentiation from human pluripotent stem cells reveals stage specific, cell line, and methodological differences.Stem Cells Transl. Med.8694–706. 10.1002/sctm.18-0267
63
MeyerJ. S.HowdenS. E.WallaceK. A.VerhoevenA. D.WrightL. S.CapowskiE. E.et al (2011). Optic vesicle-like structures derived from human pluripotent stem cells facilitate a customized approach to retinal disease treatment.Stem Cells291206–1218. 10.1002/stem.674
64
MichelD.ArsantoJ. P.Massey-HarrocheD.BéclinC.WijnholdsJ.Le BivicA. (2005). PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells.J. Cell Sci.1184049–4057. 10.1242/jcs.02528
65
NakanoT.AndoS.TakataN.KawadaM.MugurumaK.SekiguchiK.et al (2012). Self-formation of optic cups and storable stratified neural retina from human ESCs.Cell Stem Cell10771–785. 10.1016/j.stem.2012.05.009
66
Ovando-RocheP.WestE. L.BranchM. J.SampsonR. D.FernandoM.MunroP.et al (2018). Use of bioreactors for culturing human retinal organoids improves photoreceptor yields.Stem Cell Res. Ther.91–14. 10.1186/s13287-018-0907-0
67
ParkB.AlvesC. H.LundvigD. M.TanimotoN.BeckS. C.HuberG.et al (2011). PALS1 is essential for retinal pigment epithelium structure and neural retina stratification.J. Neurosci.3117230–17241. 10.1523/JNEUROSCI.4430-11.2011
68
PellikkaM.TanentzapfG.PintoM.SmithC.McGladeC. J.ReadyD. F.et al (2002). Crumbs, the Drosophila homologue of human CRB1/RP12, is essential for photoreceptor morphogenesis.Nature416143–149. 10.1038/nature721
69
PellissierL. P.AlvesC. H.QuinnP. M.VosR. M.TanimotoN.LundvigD. M. S.et al (2013). Targeted ablation of Crb1 and Crb2 in retinal progenitor cells mimics leber congenital amaurosis.PLoS Genet.9:e1003976. 10.1371/journal.pgen.1003976
70
PellissierL. P.HoekR. M.VosR. M.AartsenW. M.KlimczakR. R.HoyngS. A.et al (2014a). Specific tools for targeting and expression in Müller glial cells.Mol. Ther. Methods Clin. Dev.1:14009. 10.1038/mtm.2014.9
71
PellissierL. P.LundvigD. M. S.TanimotoN.KloosterJ.VosR. M.RichardF.et al (2014b). CRB2 acts as a modifying factor of CRB1-related retinal dystrophies in mice.Hum. Mol. Genet.233759–3771. 10.1093/hmg/ddu089
72
PellissierL. P.QuinnP. M.Henrique AlvesC.VosR. M.KloosterJ.FlanneryJ. G.et al (2015). Gene therapy into photoreceptors and Muller glial cells restores retinal structure and function in CRB1 retinitis pigmentosa mouse models.Hum. Mol. Genet.243104–3118. 10.1093/hmg/ddv062
73
PichaudF. (2018). PAR-Complex and crumbs function during photoreceptor morphogenesis and retinal degeneration.Front. Cell. Neurosci.12:90. 10.3389/fncel.2018.00090
74
PochaS. M.ShevchenkoA.KnustE. (2011). Crumbs regulates rhodopsin transport by interacting with and stabilizing myosin V.J. Cell Biol.195827–838. 10.1083/jcb.201105144
75
PolgarN.FogelgrenB. (2018). Regulation of cell polarity by exocyst-mediated traffickin.Cold Spring Harb. Perspect. Biol.10:a031401. 10.1101/cshperspect.a031401
76
QuinnP. M.AlvesC. H.KloosterJ.WijnholdsJ. (2018). CRB2 in immature photoreceptors determines the superior-inferior symmetry of the developing retina to maintain retinal structure and function.Hum. Mol. Genet.273137–3153. 10.1093/hmg/ddy194
77
QuinnP. M.BuckT. M.MulderA. A.OhoninC.AlvesC. H.VosR. M.et al (2019a). Human iPSC-derived retinas recapitulate the fetal CRB1 CRB2 Complex Formation And Demonstrate That Photoreceptors And Müller Glia Are Targets of AAV5.Stem Cell Rep.12906–919. 10.1016/j.stemcr.2019.03.002
78
QuinnP. M.MulderA. A.Henrique AlvesC.DesrosiersM.de VriesS. I.KloosterJ.et al (2019b). Loss of CRB2 in Müller glial cells modifies a CRB1-associated retinitis pigmentosa phenotype into a Leber congenital amaurosis phenotype.Hum. Mol. Genet.28105–123. 10.1093/hmg/ddy337
79
QuinnP. M.PellissierL. P.WijnholdsJ. (2017). The CRB1 complex: following the trail of crumbs to a feasible gene therapy strategy.Front. Neurosci.11:175. 10.3389/fnins.2017.00175
80
RayT. A.CochranK.KozlowskiC.WangJ.AlexanderG.CadyM. A.et al (2020). Comprehensive identification of mRNA isoforms reveals the diversity of neural cell-surface molecules with roles in retinal development and disease.Nat. Commun.11:3328. 10.1038/s41467-020-17009-7
81
RohM. H.MakarovaO.LiuC. J.ShinK.LeeS.LaurinecS.et al (2002). The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of crumbs and discs lost.J. Cell Biol.157161–172. 10.1083/jcb.200109010
82
RuedaE. M.HallB. M.HillM. C.SwintonP. G.TongX.MartinJ. F.et al (2019). The hippo pathway blocks mammalian retinal müller glial cell reprogramming.Cell Rep.271637.e6–1649.e6. 10.1016/j.celrep.2019.04.047
83
ShutovaM. V.SurdinaA. V.IschenkoD. S.NaumovV. A.BogomazovaA. N.VassinaE. M.et al (2016). An integrative analysis of reprogramming in human isogenic system identified a clone selection criterion.Cell Cycle15986–997. 10.1080/15384101.2016.1152425
84
SlavotinekA.KaylorJ.PierceH.CahrM.DewardS. J.Schneidman-DuhovnyD.et al (2015). CRB2 mutations produce a phenotype resembling congenital nephrosis, Finnish type, with cerebral ventriculomegaly and raised alpha-fetoprotein.Am. J. Hum. Genet.96162–169. 10.1016/j.ajhg.2014.11.013
85
SmithE. N.D’Antonio-ChronowskaA.GreenwaldW. W.BorjaV.AguiarL. R.PogueR.et al (2019). Human iPSC-derived retinal pigment epithelium: a model system for prioritizing and functionally characterizing causal variants at AMD risk loci.Stem Cell Rep.121342–1353. 10.1016/j.stemcr.2019.04.012
86
SuzukiA.YamanakaT.HiroseT.ManabeN.MizunoK.ShimizuM.et al (2001). Atypical protein kinase C is involved in the evolutionarily conserved PAR protein complex and plays a critical role in establishing epithelia-specific junctional structures.J. Cell Biol.1521183–1196. 10.1083/jcb.152.6.1183
87
SzymaniakA. D.MahoneyJ. E.CardosoW. V.VarelasX. (2015). Crumbs3-mediated polarity directs airway epithelial cell fate through the hippo pathway effector yap.Dev. Cell34283–296. 10.1016/j.devcel.2015.06.020
88
TaitC.ChinnaiyaK.ManningE.MurtazaM.AshtonJ.-P.FurleyN.et al (2020). Crumbs2 mediates ventricular layer remodelling to form the spinal cord central canal.PLoS Biol.18:e3000470. 10.1371/journal.pbio.3000470
89
TalibM.van SchooneveldM. J.van GenderenM. M.WijnholdsJ.FlorijnR. J.ten BrinkJ. B.et al (2017). Genotypic and phenotypic characteristics of CRB1-associated retinal dystrophies: a long-term follow-up study.Ophthalmology124884–895. 10.1016/j.ophtha.2017.01.047
90
TepassU.TheresC.KnustE. (1990). crumbs encodes an EGF-like protein expressed on apical membranes of Drosophila epithelial cells and required for organization of epithelia.Cell61787–799. 10.1016/0092-8674(90)90189-L
91
Tilston-LünelA. M.HaleyK. E.SchlechtN. F.WangY.ChattertonA. L. D.MoleirinhoS.et al (2016). Crumbs 3b promotes tight junctions in an ezrin-dependent manner in mammalian cells.J. Mol. Cell Biol.8439–455. 10.1093/jmcb/mjw020
92
TrapaniI.ColellaP.SommellaA.IodiceC.CesiG.de SimoneS.et al (2014). Effective delivery of large genes to the retina by dual AAV vectors.EMBO Mol. Med.6194–211. 10.1002/emmm.201302948
93
VallespinE.CantalapiedraD.Riveiro-AlvarezR.WilkeR.Aguirre-LambanJ.Avila-FernandezA.et al (2007). Mutation screening of 299 Spanish families with retinal dystrophies by leber congenital amaurosis genotyping microarray.Investig. Ophthalmol. Vis. Sci.485653–5661. 10.1167/iovs.07-0007
94
van de PavertS. A.KantardzhievaA.MalyshevaA.MeulemanJ.VersteegI.LeveltC.et al (2004). Crumbs homologue 1 is required for maintenance of photoreceptor cell polarization and adhesion during light exposure.J. Cell Sci.1174169–4177. 10.1242/jcs.01301
95
van de PavertS. A.MeulemanJ.MalyshevaA.AartsenW. M.VersteegI.TonagelF.et al (2007a). A single amino acid substitution (Cys249Trp) in Crb1 causes retinal degeneration and deregulates expression of pituitary tumor transforming gene Pttg1.J. Neurosci.27564–573. 10.1523/JNEUROSCI.3496-06.2007
96
van de PavertS. A.Sanz sanzA.AartsenW. M.VosR. M.VersteegI.BeckS. C.et al (2007b). Crb1 is a determinant of retinal apical muller glia cell features.Glia551486–1497. 10.1002/glia
97
van RossumA. G. S. H.AartsenW. M.MeulemanJ.KloosterJ.MalyshevaA.VersteegI.et al (2006). Pals1/Mpp5 is required for correct localization of Crb1 at the subapical region in polarized Müller glia cells.Hum. Mol. Genet.152659–2672. 10.1093/hmg/ddl194
98
VerbakelS. K.van HuetR. A. C.BoonC. J. F.den HollanderA. I.CollinR. W. J.KlaverC. C. W.et al (2018). Non-syndromic retinitis pigmentosa.Prog. Retin. Eye Res.66157–186. 10.1016/j.preteyeres.2018.03.005
99
WangD.TaiP. W. L.GaoG. (2019). Adeno-associated virus vector as a platform for gene therapy delivery.Nat. Rev. Drug Discov.18358–378. 10.1038/s41573-019-0012-9
100
WangY.RajalaA.RajalaR. V. S. (2018). Nanoparticles as delivery vehicles for the treatment of retinal degenerative diseases.Adv. Exp. Med. Biol.1074117–123. 10.1007/978-3-319-75402-4_15
101
WhitemanE. L.FanS.HarderJ. L.WaltonK. D.LiuC.-J.SoofiA.et al (2014). Crumbs3 is essential for proper epithelial development and viability.Mol. Cell. Biol.3443–56. 10.1128/mcb.00999-13
102
WhitneyD. S.PetersonF. C.KittelA. W.EgnerJ. M.PrehodaK. E.VolkmanB. F. (2016). Crumbs binding to the Par-6 CRIB-PDZ module is regulated by Cdc42.Biochemistry551455–1461.
103
YamanakaT.HorikoshiY.SuzukiA.SugiyamaY.KitamuraK.ManiwaR.et al (2001). PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induces formation of the epithelial junctional complex.Genes Cells6721–731. 10.1046/j.1365-2443.2001.00453.x
104
YuF.GuanK. (2013). The Hippo pathway: regulators and regulations.Genes Dev.27335–371. 10.1101/gad.210773.112.a
105
YuF.ZhaoB.GuanK. (2015). Hippo pathway in organ size control, tissue homeostasis, and cancer.Cell163811–828. 10.1016/j.cell.2015.10.044
106
ZahabiA.ShahbaziE.AhmadiehH.HassaniS. N.TotonchiM.TaeiA.et al (2012). A new efficient protocol for directed differentiation of retinal pigmented epithelial cells from normal and retinal disease induced pluripotent stem cells.Stem Cells Dev.212262–2272. 10.1089/scd.2011.0599
107
ZhangX.ZhangD.ChenS. C.LameyT.ThompsonJ. A.McLarenT.et al (2018). Establishment of an induced pluripotent stem cell line from a retinitis pigmentosa patient with compound heterozygous CRB1 mutation.Stem Cell Res.31147–151. 10.1016/j.scr.2018.08.001
108
ZhaoM.Andrieu-SolerC.KowalczukL.Paz CortésM.BerdugoM.DernigoghossianM.et al (2015). A new CRB1 rat mutation links müller glial cells to retinal telangiectasia.J. Neurosci.356093–6106. 10.1523/jneurosci.3412-14.2015
109
ZhongX.GutierrezC.XueT.HamptonC.VergaraM. N.CaoL. H.et al (2014). Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs.Nat. Commun.5:4047. 10.1038/ncomms5047
Summary
Keywords
retinitis pigmentosa, leber congenital amaurosis, crumbs homolog 1, gene therapy, mouse model
Citation
Boon N, Wijnholds J and Pellissier LP (2020) Research Models and Gene Augmentation Therapy for CRB1 Retinal Dystrophies. Front. Neurosci. 14:860. doi: 10.3389/fnins.2020.00860
Received
04 June 2020
Accepted
24 July 2020
Published
14 August 2020
Volume
14 - 2020
Edited by
Ayse Sahaboglu, University of Tübingen, Germany
Reviewed by
Ilaria Piano, University of Pisa, Italy; William Anthony Beltran, University of Pennsylvania, United States; Stephen Tsang, Columbia University, United States
Updates
Copyright
© 2020 Boon, Wijnholds and Pellissier.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Lucie P. Pellissier, lucie.pellissier@inrae.fr
This article was submitted to Neurodegeneration, a section of the journal Frontiers in Neuroscience
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