BRIEF RESEARCH REPORT article
Front. Neurosci.
Sec. Neurodevelopment
Volume 19 - 2025 | doi: 10.3389/fnins.2025.1589043
Foxr1 deletion causes microcephaly and leads to cortical and hippocampal hypoplasia
Provisionally accepted
- Boston University, Boston, United States
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Foxr1 is a member of the evolutionarily conserved forkhead box (Fox) family of transcription factors, characterized by a winged-helix DNA-binding domain. We previously demonstrated that Foxr1 deletion in mice results in severe perinatal lethality, cortical thinning, and ventricular enlargement, indicating its essential role in survival and brain development. Here, we extend these findings by showing that Foxr1 knockout mice develop microcephaly accompanied by cortical and hippocampal hypoplasia at postnatal day 0 (P0). Cortical thinning is primarily driven by a significant reduction in layer 2/3 neurons, linked to impaired generation of later-born neurons. This reduction correlates with decreased proliferation of progenitors (Ki67- and Tbr2-positive cells) at embryonic day 16.5 (E16.5), a critical period for upper-layer neurogenesis. In the hippocampus, Foxr1 knockouts exhibit reduced area, and cell counts at P0, accompanied by increased proliferation (Ki67-positive cells), and elevated apoptosis (CC3-positive) at E16.5, suggesting broader disruptions in progenitor maintenance. Together, these findings suggest Foxr1 is an important regulator of progenitor dynamics and neuron production in cortical and hippocampal development.
Keywords: foxr1, Transcription Factors, Microcephaly, Cortex, Mouse
Received: 06 Mar 2025; Accepted: 12 May 2025.
Copyright: © 2025 Waxman, Kankkunen, Gupta, Dowgiewicz, Beffert and Ho. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Angela Ho, Boston University, Boston, United States
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