ORIGINAL RESEARCH article
Front. Neurosci.
Sec. Neurodegeneration
Volume 19 - 2025 | doi: 10.3389/fnins.2025.1595847
This article is part of the Research TopicMultiomics Approaches in the Central Nervous System and NeuropathiesView all 3 articles
Expression and potential regulatory mechanism of cellular senescencerelated genes in Alzheimer's disease based on single-cell and bulk RNA datasets
Provisionally accepted
- 1Department of General Practice, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, Nanjing, China
- 2Department of General Practice, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Liaoning Province, China
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Alzheimer's disease (AD) is the most common cause of dementia in the elderly. However, the particular cause of AD development has not been fully elucidated. Currently, cellular senescence is recognized as a contributing factor to the aging process and age-related diseases. The present study aimed to identify the hinge of regulatory factors in dysfunctional cellular senescence genes in AD via integrating multiple omics analysis, including single-cell RNA sequencing and bulk sequencing data. In addition, UMAP scatter diagrams were constructed, while active cell subtypes and pathways involved in cellular senescence were identified via performing Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, respectively. The results indicated that a total of seven clusters were detected by known marker genes, including excitatory neurons, inhibitory neurons, astrocytes, microglial cells, oligodendrocytes, oligodendrocyte progenitor cells and pericytes/endothelial cells. CDK18 was specifically expressed in oligodendrocytes, RUNX1 in microglia, SORBS2 and KSR2 in neurons, PDZD2 in oligodendrocyte progenitors, YAP1 in astrocytes and NOTCH3 in pericytes/endothelial cells. Astrocytes, microglia, and pericytes/endothelial cells were found to be the most active cell subtypes. AD-associated cellular senescence genes in the Astrocytes subgroup(SOX5, AR, HMGB1, NR2E1, ID4, TP53, MXD4, FOS, BHLHE40, PIVEP1), microglia subgroup (BCL6, ETS2, CEBPB, MXD4, FOS, NFE2L2, FOXO3, IRF3, PBRM1, RUNX1, IRF5, ZNF148) and pericyte/endothelial cell subgroup (SOX5, BCL6, ETS2, CEBPB, FOS, TP63, TBX2, ETS1, BHLHE40, ID1) were identified. Furthermore, potential therapeutic targets and drugs for AD were identified via analyzing the molecular mechanisms and pathways involved in cellular senescence. The above findings demonstrated that cellular senescence could play a crucial role in the pathogenesis of AD and highlighted the significance of understanding the role of cellular senescence in the pathogenesis of AD. The results of the current study could provide novel insights into the development of potential therapeutic targets and pave the way for the development of novel therapeutic strategies for AD.
Keywords: Alzheimer's disease, cellular senescence, gene, Single-cell RNA, bulk RNA datasets AR, HMGB1, Nr2e1, Id4
Received: 18 Mar 2025; Accepted: 06 May 2025.
Copyright: © 2025 Sha, Zhang, Fang, Wang, He and Xin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dujuan Sha, Department of General Practice, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, Nanjing, China
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