REVIEW article
Front. Neurosci.
Sec. Neuroscience Methods and Techniques
Volume 19 - 2025 | doi: 10.3389/fnins.2025.1597151
This article is part of the Research TopicIntegrating Multimodal Approaches to Unravel Neural Mechanisms of Learning and CognitionView all 8 articles
Advances in two-photon imaging for monitoring neural activity in behaving mice
Provisionally accepted
- 1Institute of Neuroscience, College of Medicine, Panzhihua University, Panzhihua, China
- 2The Functional Science laboratory, College of Medicine, Panzhihua University,, Panzhihua, China
- 3Panzhihua University, Panzhihua, China
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An essential goal in neuroscience is to establish a link between animal behavior and neural activity.Monitoring neural activity during behavior is enhanced by establishing simple and consistent behaviors associated with perceptual cues. In recent years, significant strides have been made in studying the neural activity of behaving mice, thanks to the concurrent advancements in imaging systems and the development of multiple indicators. This review summarizes the current applications and methodological advancements of two-photon imaging technology in the context of behavioral mouse studies and explores the broader potential for applying this technology in future research with behaving mice.
Keywords: Neuroscience, Behaving mice, two-photon imaging technology, indicator, advances
Received: 20 Mar 2025; Accepted: 30 Jul 2025.
Copyright: © 2025 Li, Li, Zhu, Shi, Shen and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Yi Zhang, Panzhihua University, Panzhihua, China
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