ORIGINAL RESEARCH article
Front. Neurosci.
Sec. Neuropharmacology
Volume 19 - 2025 | doi: 10.3389/fnins.2025.1601101
This article is part of the Research TopicNew Strategies for Spinal Cord Injury and Immunotherapy Targeting Novel Programmed Death PathwaysView all 7 articles
Inhibition of miR-20a Promotes Neural Stem Cell Survival Under Oxidative Stress Conditions
Provisionally accepted
- 1Institute of Experimental Medicine (ASCR), Prague, Czechia
- 2Second Faculty of Medicine, Charles University, Prague, Prague, Czechia
- 3Institute of Biotechnology (ASCR), Vestec, Czechia
- 4Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Prague, Prague, Czechia
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Oxidative stress (OS) is a critical factor in neural degeneration and impaired neuronal stem cell (NSC) survival. MicroRNA-20a (miR-20a) plays a significant role in cellular stress responses, including apoptosis and survival pathways. This study investigated the effects of inhibiting miR-20a on NSC survival and metabolic activity following OS induced by hydrogen peroxide (H2O2). NSCs exposed to 100 µM H2O2 showed a marked reduction in metabolic activity. However, treatment with a miR-20a inhibitor (100 nM) over 72 hours significantly improved cell survival and metabolic activity in a time-dependent manner compared to untreated stressed cells. These findings suggest that miR-20a inhibition mitigates OS-induced cytotoxicity and promotes NSC viability, presenting a potential therapeutic approach for enhancing neural tissue regeneration.
Keywords: MicroRNA-20a, Neural Stem Cells, Oxidative Stress, Neuronal apoptosis, Neuroprotection
Received: 27 Mar 2025; Accepted: 11 Jun 2025.
Copyright: © 2025 Arzhanov, Klassen, Valihrach and Romanyuk. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Nataliya Romanyuk, Institute of Experimental Medicine (ASCR), Prague, Czechia
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