Your new experience awaits. Try the new design now and help us make it even better

METHODS article

Front. Neurosci.

Sec. Neuroscience Methods and Techniques

Volume 19 - 2025 | doi: 10.3389/fnins.2025.1601127

Optimized workflow for behavior-coupled fiber photometry experiment: improved data navigation and accessibility

Provisionally accepted
  • 1Centre de Recherche en Neurosciences de Lyon, Bron, France
  • 2UMR5242 Institut de Génomique Fonctionnelle de Lyon (IGFL), Lyon, Rhône-Alpes, France

The final, formatted version of the article will be published soon.

Fiber photometry provides crucial insights into cell population activity underlying behavior. While numerous open-source data analysis tools exist, few offer an automated workflow that streamlines the analysis of fiber photometry data alongside behavioral measurements, by enabling more intuitive and facilitated navigation within data files. We developed here a workflow starting from the intracerebral implantation of optical fibers in mice, to the analysis of fiber photometry signals, aligned with recorded behavioral data. This tool is particularly valuable for studying unpredictable exploratory behaviors, as it allows efficient and rapid revisiting of fiber photometry signals aligned to spontaneous behavioral changes. Our approach allows ease of data analysis and exploration using custom algorithms and scripts that extract and process both fiber photometry and behavioral data, without relying on predefined event markers.We validated our method by assessing calcium activity and dopaminergic dynamics in the olfactory tubercle in response to spontaneous investigation of attractive and non-attractive odorants in freely moving adult C57BL/6J mice. Using jRGECO1a and dLight1.2 biosensors, we revealed distinct dopamine responses to attractive versus unattractive odorants while calcium transmission remained similar. Overall, our method significantly enhances the accessibility and efficiency of data analysis, allowing for rapid retrieval and exploration of key behavioral time points. Its adaptability makes it suitable for a wide range of spontaneous behaviors, paradigms, and sensory modalities, facilitating deeper insights into complex neural dynamics.

Keywords: Fiber Photometry, Video Recording, Behavior, Olfactory Perception, Mice

Received: 27 Mar 2025; Accepted: 30 Jun 2025.

Copyright: © 2025 Athanassi, François, Bourinet, THEVENET and Mandairon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Anna Athanassi, Centre de Recherche en Neurosciences de Lyon, Bron, France
Nathalie Mandairon, Centre de Recherche en Neurosciences de Lyon, Bron, France

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.