ORIGINAL RESEARCH article
Front. Neurosci.
Sec. Translational Neuroscience
Volume 19 - 2025 | doi: 10.3389/fnins.2025.1622760
This article is part of the Research TopicRecent Advances in Translational Neurovascular and Cerebroprotection ResearchView all 5 articles
Exploring ischemic stroke based on the ferroptosis perspective: ECH1 may serve as a new biomarker and therapeutic target
Provisionally accepted
- Beijing University of Chinese Medicine, Beijing, China
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Background: Ischemic stroke (IS), the leading stroke subtype (~87%), arises from vascular occlusions, triggering brain necrosis through ischemia-reperfusion injury. Ferroptosis, an iron-driven cell death via Fe²⁺-mediated lipid peroxidation, is implicated in IS pathology. This study demonstrates that enoyl-coA hydrolase 1 (ECH1) may serve as a peripheral biomarker and therapeutic target for IS based on ferroptosis signaling. Methods: We integrated transcriptome data from the GEO database with preprocessing and normalization. Hub genes were screened using differential expression analysis and machine learning algorithms. Subsequently, genes were further filtered by mendelian randomization and also validated using transient middle cerebral artery occlusion (tMCAO) model. Results: Ferroptosis-related genes in the IS group showed higher expression compared with the healthy control group. Using differential expression analysis and machine learning algorithms, 12 potential hub genes were successfully screened. Mendelian randomization analysis further confirmed the causal association between ECH1 and stroke. In the tMCAO mouse model, ECH1 mRNA levels were down-regulated, consistent with the results of the clinical samples. Conclusion: In this study, taking ferroptosis as an entry point, ECH1 may serve as a potential peripheral blood biomarker and therapeutic target for IS through multidimensional validation, providing a basis for the development of relevant precision diagnostic strategies.
Keywords: ischemic stroke, ferroptosis, Peripheral Blood, ECH1, TMCAO
Received: 04 May 2025; Accepted: 28 Jul 2025.
Copyright: © 2025 Qu, Zhang, Zhang, Li, Zhang, Tong and Lu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Rendong Qu, Beijing University of Chinese Medicine, Beijing, China
Hongxuan Tong, Beijing University of Chinese Medicine, Beijing, China
Tao Lu, Beijing University of Chinese Medicine, Beijing, China
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