Non-invasive brain stimulation for stroke-related motor impairment and disability: an umbrella review of systematic review and meta-analysis

Introduction: Non-invasive brain stimulation (NIBS) techniques, particularly repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS), have shown potential in stroke rehabilitation. However, systematic reviews often reach conflicting conclusions, highlighting the need for an umbrella review.

Objective: To synthesize, based on the principal domains of the International Classification of Functioning, Disability and Health (ICF) framework, the best available evidence on the effectiveness and safety of NIBS for improving motor impairment and disability after stroke.

Methods: We conducted an umbrella review (PROSPERO: CRD42021239577) that included meta-analyses of controlled trials investigating NIBS effects in stroke survivors, retrieved from PubMed/MEDLINE from February 2020 to July 2025. Methodological quality was appraised using AMSTAR-2 and certainty of evidence using GRADE. Outcomes were mapped to ICF body structure/function and activity domains.

Results: Fifty-six studies were included (2–48 primary trials each; 54–1,654 participants per meta-analysis). All included studies evaluated only rTMS and tDCS; no meta-analyses of other NIBS modalities met inclusion criteria. Methodological quality was high or moderate in 85.7% of the meta-analyses. Certainty of evidence was low or very low for 14/50 studies; only one rTMS review provided moderate-certainty evidence for activities of daily living. rTMS showed improvement in activities of daily living (ADL; SMD = −0.82, 95% CI −1.05 to −0.59), upper-limb motor impairment (SMD = −0.32, 95% CI −0.55 to −0.09) and variable effects on mobility from small (SMD = −0.35, 95% CI −0.45 to −0.24) to large (SMD = −0.97, 95% CI −1.28 to −0.66). tDCS was supported by very-low-certainty evidence: small effects were found for motor impairment (SMD = −0.22, 95 % CI −0.32 to −0.12) and upper-limb activity (SMD = −0.31, 95% CI −0.55 to −0.01), while a much smaller subset of trials suggested a large effect (SMD = −1.54, 95% CI −2.78 to −0.29). Effects on ADL and mobility with tDCS were inconsistent and generally non-significant.

Conclusion: rTMS was more frequently associated with moderate to large effect sizes for body structure/function outcomes, particularly general neurological function. In contrast, tDCS demonstrated small effects on motor recovery, though evidence certainty was very low due to heterogeneity, imprecision, and protocol variability. Within the activity domain, NIBS showed modest effects, with rTMS showing more consistent benefits for ADL. tDCS effects were generally limited and supported by low to very low certainty of evidence.

Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/view/CRD42021239577.

1 Introduction

Stroke is a leading cause of motor impairment and disability worldwide, consistently exerting a significant impact on public health across many countries (Virani et al., 2021). Non-invasive brain stimulation (NIBS) is a set of techniques that apply non-invasively electromagnetically-induced currents to modulate the excitability of the targeted brain areas and their networks (Brunoni et al., 2019). NIBS approaches might enhance or drive adaptive plastic changes in the central nervous system (CNS) for the management of various stroke-related sensorimotor impairments and disabilities, including spasticity (Graef et al., 2016; McIntyre et al., 2018), upper or lower motor function (Zhang et al., 2017a; Kang et al., 2018; Vaz et al., 2019), balance impairments (Li et al., 2018a; Ghayour-Najafabadi et al., 2019; Kang et al., 2020; Tien et al., 2020), mobility (Ghayour-Najafabadi et al., 2019; Tien et al., 2020) and difficulties with activities of daily living (Subramanian and Prasanna, 2018; Xiang et al., 2019; Ahmed et al., 2022).

In recent decades, NIBS has been proposed as a possible adjuvant strategy to augment the efficacy of conventional rehabilitation treatments for sensorimotor impairments in neurological populations (Liew et al., 2014). In the context of stroke rehabilitation, several NIBS modalities have been investigated (Kim and Park, 2024; Shen et al., 2022). However, repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS) have been the most extensively studied (Mahmoud et al., 2024). Although the quality of available evidence remains limited, numerous clinical studies suggest that NIBS holds promise for enhancing motor recovery after stroke. Recently, several systematic reviews have synthesized the growing body of evidence on NIBS (Qi et al., 2024; Barreto et al., 2025). However, the large number of available reviews may lead to conflicting conclusions and hinder consensus on the effectiveness of NIBS. To address this challenge, umbrella reviews have become increasingly important, providing a qualitative meta-synthesis of systematic reviews or meta-analyses. By synthesizing evidence across multiple reviews, umbrella reviews can help resolve inconsistencies and provide a comprehensive overview of findings. Thus, they are considered one of the highest levels of evidence synthesis currently available and have been used to inform the adoption of specific clinical techniques in practice (Aromataris et al., 2015; Liu et al., 2020).

Another important limitation of most existing reviews on NIBS for post-stroke motor recovery is their predominant focus on isolated clinical outcomes (e.g., motor scores or spasticity), without contextualizing the findings within a comprehensive functional framework that reflects real-world functioning. The International Classification of Functioning, Disability and Health (ICF) provides a comprehensive framework to address this gap by categorizing the consequences of stroke-related neurological damage across three core domains: impairments in body structures and functions, limitations in activity, and restrictions in participation (Leonardi and Fheodoroff, 2021). The ICF has become a standard for understanding and categorizing the multidimensional impact of health conditions such as stroke (Virani et al., 2021; The Lancet, 2019; Stucki et al., 2007).

In this context, we conducted an umbrella review to summarize the evidence on the use of NIBS techniques for motor recovery and disability reduction in stroke survivors, framing the synthesis within the core domains of the ICF. We conceptualize motor recovery as a multidimensional process that encompasses improvements in body structures and functions, as well as gains in activity performance. This umbrella review aims to enhance the clinical relevance of the synthesized findings and support more holistic interpretations of NIBS effects.

2 Materials and methods

2.1 Study design

This review is part of a series of umbrella reviews produced by the Working-Group on scientific evidence for the use of non-invasive brain stimulation within the NIBS Brazilian Guidelines Development Group of the NAPeN Network (http://www.neuromodulation-net.com). The protocol was registered in PROSPERO (CRD42021239577; February/2020) and subsequently published by Shirahige et al. (2022), following the recommendations of the preferred reporting items for overviews of reviews (PRIOR) statement (Gates et al., 2022).

2.2 Search and eligibility criteria

Two independent reviewers (BR and PL) conducted a comprehensive literature search from April 2020 to July 2025 in PubMed/MEDLINE. Disagreements during the screening process were resolved through discussion to reach a consensus; if consensus could not be achieved, a third reviewer (LS) was consulted. The search strategy was developed and validated in consultation with specialists in scientific methodology and experts in NIBS. These experts reviewed the selection of keywords, controlled vocabulary terms, and Boolean operators to ensure the adequacy, sensitivity, and specificity of the search process in line with the aims of this umbrella review.

To enhance the comprehensiveness of the meta-analysis, the snowball method was also employed, identifying additional relevant studies through reference lists and forward citation tracking, thereby ensuring comprehensive inclusion of pertinent literature. We included meta-analyses of controlled trials (CTs) involving any NIBS technique used as a treatment for motor impairments and disability in stroke survivors. Searches were conducted using Medical Subject Headings (MeSH) terms. The NIBS techniques included were: transcranial direct current stimulation (tDCS), transcranial alternating current stimulation (tACS), transcranial random noise stimulation (tRNS), transcutaneous spinal direct current stimulation (tsDCS), transcutaneous auricular vagus nerve stimulation (taVNS), high-definition transcranial direct current stimulation (HD-tDCS), and repetitive transcranial magnetic stimulation (rTMS). The scope of the search was guided by the list of electrical and magnetic NIBS modalities most frequently reported in the scientific literature, as outlined by experts from the NAPeN Network Group. Eligibility criteria are summarized in Box 1. All strategies including respective MeSH terms and number of retrieved articles are described in Supplementary Table 1.

Box 1

Box 1. Eligibility criteria for considering articles for the umbrella review.

2.3 Study selection and data extraction

Titles, abstracts and full texts were screened by two independent reviewers (BR and PL) to assess study eligibility. Disagreements during screening were resolved through discussion to reach consensus; if consensus could not be reached, a third reviewer (LS) was consulted. The following data were extracted from each included study: (1) author/year of publication; (2) characteristics of patients from selected articles; (3) intervention protocols used in the articles; (4) number of patients, number of patients included in the meta-analysis, heterogeneity index, and p-value; (5) adverse effects: tissue damage, behavioral changes and vasovagal syncope; (6) outcome measures used in each meta-analysis.

Severe adverse events included incapacitating headaches, seizures, syncope, psychiatric and cognitive/neuropsychological changes, and tissue injury. Results of each meta-analysis were extracted separately for each outcome. All data were checked to ensure accuracy and consistency in two steps. Any discrepancies were resolved by consensus. Outcome measures were classified according to the principal domains of the ICF conceptual framework, body structure/function and activity, based on the approach proposed by Salter et al. (2019). In addition, we specified all outcome measures used in the included studies within the corresponding ICF subdomains, as detailed in Supplementary Table 2.

2.4 Assessment of meta-analyses methodological quality

The quality of the included systematic reviews was assessed using the AMSTAR-2 (A Measurement Tool to Assess Systematic Reviews) checklist (Shea et al., 2017). This instrument evaluates 16 domains that evaluate methodological quality. Specifically, the following aspects were considered: inclusion of PICO components in the research question and eligibility criteria (Item 1); prospective registration of the review protocol and justification for deviations (Item 2); justification for the selection of study designs (Item 3); use of a comprehensive literature search strategy (Item 4); study selection performed in duplicate (Item 5); data extraction performed in duplicate (Item 6); listing and justification of excluded studies (Item 7); adequate description of included studies (Item 8); appropriate assessment of risk of bias in individual studies (Item 9); reporting of funding sources for the included studies (Item 10); use of appropriate methods for statistical combination of results (Item 11); consideration of risk of bias when interpreting results of the meta-analysis (Item 12); consideration of risk of bias in the discussion or interpretation of the review findings (Item 13); explanation of heterogeneity in the review results (Item 14); assessment of publication bias and its potential impact on findings (Item 15); and disclosure of conflicts of interest and funding for the review itself (Item 16).

Each item was rated as “Yes,” “Partially yes,” or “No,” with emphasis placed on seven critical items that significantly impact the overall score (Shea et al., 2017). The quality of each included meta-analysis was assessed by considering non-critical items (1, 3, 5, 6, 8, 10, 12, 14, and 16) and critical items (2, 4, 7, 9, 11, 13, and 15).

Based on ratings for critical and non-critical items, the systematic reviews were categorized into one of four quality levels: “high quality” (no or one non-critical weakness), “moderate quality” (more than one non-critical weakness), “low quality” (one critical flaw with or without non-critical weaknesses), and “critically low” (more than one critical flaw with or without non-critical weaknesses; Shea et al., 2017). Methodological quality assessments were performed independently by two researchers. Any disagreements were resolved through discussion, and if consensus was not reached, a third reviewer was consulted.

2.5 Assessment of evidence quality

Data were extracted into Summary of Finding tables using GRADEpro GDT (Grading of Recommendations Assessment, Development and Evaluation Guideline Development Tool; http://www.gradepro.org). Data was organized according to the main domains of the ICF. Separate tables were created for each NIBS technique addressing outcomes of ICF body structure/function and activity. The GRADE approach provides a quality rating for each outcome as high, moderate, low, or very low. High-quality evidence indicates that future studies are unlikely to change the effect size estimate; moderate-quality evidence suggests that future RCTs may have an impact on the effect size estimate; low-quality evidence indicates that there is a high probability that future studies will change the effect size estimate; and very low-quality evidence indicates that there is very little certainty about the effect size estimate.

2.6 Statistical analysis

Given the considerable variability in the NIBS protocols across studies and the use of different instruments to assess body structure/function, we used the standardized mean difference (SMD) as the treatment effect for continuous outcome measures. This approach allowed for the standardization of results across studies. Pooled SMDs were calculated as the overall treatment effect size in the meta-analyses (Gallardo-Gómez et al., 2024). We interpreted pooled SMDs using rules of thumb (< 0.40 = small, 0.40–0.70 = moderate, >0.70 = large effect) according to the Cochrane Handbook for Systematic Reviews of Interventions (Higgins et al., 2024).

When original meta-analyses reported outcomes only as mean differences, we re-analyzed the post-intervention data by extracting the mean and standard deviation (SD) from each included study and generated new forest plots using SMDs. If means and SDs were not provided, median values were considered to be equal to mean values if data were normally distributed, and interquartile ranges were divided by 1.35 to obtain the SD (Higgins and Welch, n.d.). When necessary, we also derived the SD from confidence intervals, following the Cochrane Handbook (Higgins and Welch, n.d.). When the study only presented the results in graphs, we extracted the data using WebPlotDigitizer (available at https://apps.automeris.io/wpd/). All adjusted meta-analyses were performed using RevMan 5 software (Cochrane Information Management System).

To enhance the clinical interpretability of the effect sizes reported in our systematic review, we converted standardized mean differences (SMDs) into approximate estimates of the Number Needed to Treat (NNT), following the approach proposed by Furukawa and Leucht (2011). The conversion was performed using the following formula:

$\begin{array}{l}\text{NNT}=\frac{1}{\text{Φ}\left(\frac{\text{SMD}}{\sqrt{2}}\right)}-0.5\end{array}$

Where Φ is the cumulative distribution function (CDF) of the standard normal distribution, and SMD is the standardized mean difference for the outcome of interest. This approach allows for a rough but informative approximation of NNT from continuous outcomes. The resulting NNT values, along with their corresponding 95% confidence interval (NNT lower and higher), were added to a Supplementary Table 2 alongside the original SMD, to support clinical interpretation. Negative SMDs, where applicable, were interpreted in the context of the direction of benefit, and the sign was adjusted accordingly when calculating NNT.

All statistical tests were two-sided, with significance set at p ≤ 0.05. Homogeneity was evaluated by a heterogeneity test. A meta-analysis was considered homogeneous when the p-value was >0.05 and the heterogeneity index (I²) was up to 30%. When heterogeneity was >30%, a random-effects model was used, whereas a fixed-effect model was used when I² was ≤ 30%. The Supplementary Table 2 provides the specific measure considered for each main domain of the ICF included in the meta-analyses.

3 Results

3.1 Study selection and characteristics of included meta-analyses

A total of 56 systematic reviews with meta-analysis met the eligibility criteria and were included in the study. All retrieved studies focused exclusively on the efficacy of rTMS and tDCS, with no eligible meta-analyses found for other NIBS modalities. The screening strategy is shown in the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) flowchart presented in Figure 1.

Figure 1

Figure 1. PRISMA flowchart.

The included studies were published between 2016 (Elsner et al., 2016) and 2025 (Barreto et al., 2025). Of the 56 studies included, 35 evaluated the efficacy of rTMS (Graef et al., 2016; Shen et al., 2017; Wang et al., 2025; Zhang et al., 2017a,b; Li et al., 2018a; McIntyre et al., 2018; Ghayour-Najafabadi et al., 2019; Liu et al., 2019; Tung et al., 2019; van Lieshout et al., 2019; Xiang et al., 2019; Allida et al., 2020; Shao et al., 2021; Krogh et al., 2022; Gao et al., 2023; Hofmeijer et al., 2023; Chen X. et al., 2023; Chen Y. et al., 2023; Xie et al., 2023, 2025; Xi et al., 2023; Zhou et al., 2023; Chen et al., 2024; Daoud et al., 2024; Jiang et al., 2024; Zhang J. J. et al., 2024; Wang J. et al., 2024; Wang Y. et al., 2024; Zeng et al., 2024; Zhu et al., 2024; Barreto et al., 2025; Jia et al., 2025; Ma et al., 2025; Zhang et al., 2025) whereas 16 evaluated that of tDCS (Elsner et al., 2016, 2020; Tedesco Triccas et al., 2016; Li et al., 2018b; Tien et al., 2020; Van Hoornweder et al., 2021; Comino-Suárez et al., 2021; Reis et al., 2021; Sun et al., 2021; Huang et al., 2022; Lima et al., 2023, 2024; Zhang N. et al., 2024; Tang et al., 2024; Usman et al., 2024; Yu et al., 2025). Notably, 5 meta-analyses evaluated both rTMS and tDCS within the same review (O'Brien et al., 2018; Vaz et al., 2019; Kang et al., 2020; Ahmed et al., 2023; Ren et al., 2024). The number of primary studies included in each meta-analysis ranged from 2 (McIntyre et al., 2018; Allida et al., 2020; Ahmed et al., 2023) to 48 (Zhou et al., 2023), and the number of participants ranged from 54 (Xi et al., 2023) to 1654 (Xie et al., 2025).

Control interventions included sham stimulation, or no intervention associated with physiotherapy, occupational therapy, task-oriented training, mirror therapy, treadmill training, usual care, constraint-induced movement therapy, or pharmacological interventions. The characteristics of the included meta-analyses are summarized in Table 1.

Table 1

Table 1. Main characteristics of the included meta-analyses.

3.2 Results of the methodological quality (AMSTAR)

AMSTAR scores ranged from 8 (McIntyre et al., 2018; Kang et al., 2020; Sun et al., 2021) to 16 points (Allida et al., 2020; Elsner et al., 2020; Barreto et al., 2025). Twenty three studies (41.1%) were considered to be of high quality (Elsner et al., 2016, 2020; O'Brien et al., 2018; Liu et al., 2019; Vaz et al., 2019; Allida et al., 2020; Comino-Suárez et al., 2021; Reis et al., 2021; Shao et al., 2021; Huang et al., 2022; Krogh et al., 2022; Gao et al., 2023; Lima et al., 2023; Xie et al., 2023; Jiang et al., 2024; Lima et al., 2024; Zhang N. et al., 2024; Tang et al., 2024; Usman et al., 2024; Zeng et al., 2024; Barreto et al., 2025; Jia et al., 2025), 25 studies (44.6%) were considered to be of moderate quality (Graef et al., 2016; Tedesco Triccas et al., 2016; Zhang et al., 2017a; Li et al., 2018a,b; Ghayour-Najafabadi et al., 2019; Tung et al., 2019; van Lieshout et al., 2019; Xiang et al., 2019; Tien et al., 2020; Hofmeijer et al., 2023; Xi et al., 2023; Chen X. et al., 2023; Zhou et al., 2023; Chen et al., 2024; Daoud et al., 2024; Zhang J. J. et al., 2024; Wang J. et al., 2024; Ren et al., 2024; Wang Y. et al., 2024; Wang et al., 2025; Ma et al., 2025; Xie et al., 2025; Zhang et al., 2025), two study (3.6%) were considered as “low quality”(Zhang et al., 2017b; Zhu et al., 2024), and 6 studies (10.7%) were classified as critically low quality (McIntyre et al., 2018; Kang et al., 2020; Sun et al., 2021; Ahmed et al., 2023; Chen Y. et al., 2023; Yu et al., 2025).

The items with the highest scores across reviews were: “PICO Components” (item 1); “study designs for inclusion in the review” (item 3); “perform data extraction in duplicate” (item 6); risk of bias (RoB) in individual studies that were included in the review” (item 9); “appropriate methods for statistical combination of results” (item 11); “quantitative synthesis did the review authors carry out an adequate investigation of publication bias” (item 15) and “potential sources of conflict of interest, including any funding they received for conducting the review” (item 16). Conversely, the items with the highest proportion of studies presenting risk of bias were “whether the review and report justified any significant deviation from the protocol” (item 2); “authors use a comprehensive literature search strategy” (item 4) and “funding for the studies included in the review” (item 10). The AMSTAR ratings are presented in Table 2.

Table 2

Table 2. AMSTAR ratings.

3.3 Grading of evidence results (GRADE)

Based on the GRADE assessment, we categorized the evidence according to each NIBS technique. Tables 3a, 3b present a Summary of Findings (SoF) and evidence quality for each meta-analysis on rTMS for body structure/function and activity domains, respectively, while Tables 4a, 4b provide the same for tDCS meta-analyses.

Table 3a

Table 3a. Summary of findings (SoF) and certainty of evidence regarding included studies that investigated the effects of repetitive transcranial magnetic stimulation (rTMS) in ICF body structure and function domains.

Table 3b

Table 3b. Summary of findings (SoF) and certainty of evidence regarding included studies that investigated the effects of repetitive transcranial magnetic stimulation (rTMS) in ICF activity and participation domains.

Table 4a

Table 4a. Summary of findings (SoF) and certainty of evidence regarding included studies that investigated the effects of transcranial direct current stimulation (tDCS) in ICF body structure and function domains.

Table 4b

Table 4b. Summary of findings (SoF) and certainty of evidence regarding included studies that investigated the effects of transcranial direct current stimulation (tDCS) in ICF activity and participation domains.

For rTMS, the majority of meta-analyses were rated as low or very low certainty of evidence, with the exception of Xiang et al. (2019), which evaluated rTMS effects on activities of daily living (ADL) post-stroke and were rated as having moderate certainty of evidence. For tDCS, all studies demonstrated low or very low certainty of evidence. Many of the meta-analyses showed inconsistencies due to high variability in NIBS protocols and/or imprecision in results, attributed to small effect sizes or broad confidence intervals.

3.4 Efficacy of rTMS for body structure/function

Figures 2, 3 summarize the clinical efficacy of rTMS across meta-analyses, mapped according to SMD, methodological quality (AMSTAR score), outcome domain (classified according to the ICF framework), sample size, stimulation protocol, and adverse event reporting. Figure 2 presents outcomes related to body structure/function, while Figure 3, discussed in Section 3.5, refers to activity.

Figure 2

Figure 2. Evidence map for the use of repetitive transcranial magnetic stimulation (rTMS) on body structure and function. “Mixed protocols” indicates that various non-invasive brain stimulation (NIBS) protocols were included within the same meta-analysis.

Figure 3

Figure 3. Evidence map for the use of repetitive transcranial magnetic stimulation (rTMS) on activity. “Mixed protocols” indicates that various non-invasive brain stimulation (NIBS) protocols were included within the same meta-analysis.

Among the 56 included meta-analyses, 32 meta-analyses (57.1%), including between 2 RCT (56 patients; McIntyre et al., 2018) and 36 RCT (1654 patients; Xie et al., 2025), investigated the effect of rTMS on body structure and function domain of the ICF framework, primarily targeting motor function. These studies were categorized into two main outcome domains: motor function (mainly assessed through the Fugl-Meyer Assessment for upper limb- FMA-UL, Fugl-Meyer Assessment for Lower Limb—FMA-LL), and general neurological function (assessed through the National Institutes of Health Stroke Scale—NIHSS).

Of 26 meta-analyses that investigated the effects of rTMS in motor function, 16 (61.5%) observed that rTMS was effective in improving motor function after stroke (Zhang et al., 2017b; Tung et al., 2019; van Lieshout et al., 2019; Xiang et al., 2019; Hofmeijer et al., 2023; Xi et al., 2023; Chen Y. et al., 2023; Jiang et al., 2024; Zhang J. J. et al., 2024; Wang Y. et al., 2024; Zeng et al., 2024; Barreto et al., 2025; Jia et al., 2025; Ma et al., 2025; Xie et al., 2025; Zhang et al., 2025). These studies varied from a moderate (SMD: −0.45; CI: −0.65 to −0.25; Table 3a; Jia et al., 2025) to high (SMD: −1.22; CI: −0.73 to −1.70; Table 3a) effect size (Chen X. et al., 2023; Figure 2). The NNT varied from 8 to 3 (Supplementary Table 2). In general, meta-analyses with larger sample sizes appeared to report results that are more favorable toward rTMS. Notably, the studies by Chen X. et al. (2023), (Xi et al. 2023), (Hofmeijer et al. 2023), and (Ma et al. 2025) reported the largest effect sizes. Five studies were classified as “low quality” of evidence for motor function (Xiang et al., 2019; Xi et al., 2023; Jiang et al., 2024; Barreto et al., 2025; Jia et al., 2025).

Six meta-analyses (100%) reported that general neurological function was slightly (Liu et al., 2019; Allida et al., 2020; Shao et al., 2021; Chen Y. et al., 2023) or potentially meaningful improvements after rTMS treatment (Figure 2). These studies varied from a moderate (SMD: −0.67; CI: −1.02 to −0.32, Table 3a; Shao et al., 2021) to high (SMD: −2.21; CI: −3.32 to −1.09, Table 3a; Liu et al., 2019; Allida et al., 2020) effect size. The NNT varied from 5 to 3 (Supplementary Table 2). However, four of these studies reported high inconsistency and used control groups that included only medication intake, which contributed to their classification as “very low quality of evidence” (Tables 1, 3a). Only two studies were classified as “low quality of evidence” for general neurologic function (Liu et al., 2019; Shao et al., 2021).

3.5 Efficacy of rTMS for stroke activity

Thirty-nine meta-analyses, including between 2 studies (128 patients; Ahmed et al., 2023) and 20 studies (825 patients; Xie et al., 2025), investigated the effect of rTMS on outcomes related to ICF domain of activity (Figure 3). The outcomes considered from the studies comprised: (1) upper limb activities; (2) mobility, and (3) ADL. Full details of the outcome measures are available in Table 1.

Of the 39 studies, seven (17.9%) investigated the upper limb activity (Graef et al., 2016; Zhang et al., 2017b; O'Brien et al., 2018; van Lieshout et al., 2019; Chen X. et al., 2023; Xi et al., 2023; Jiang et al., 2024; Zhang J. J. et al., 2024), 18 studies (46.2%) investigated performance in ADL (Liu et al., 2019; Xiang et al., 2019; Allida et al., 2020; Ahmed et al., 2023; Gao et al., 2023; Hofmeijer et al., 2023; Chen Y. et al., 2023; Xie et al., 2023, 2025; Xi et al., 2023; Chen et al., 2024; Daoud et al., 2024; Jiang et al., 2024; Ren et al., 2024; Wang J. et al., 2024; Wang Y. et al., 2024; Zhu et al., 2024; Wang et al., 2025) and 14 (35.9%) focused on mobility (Figure 3; Li et al., 2018a; Ghayour-Najafabadi et al., 2019; Tung et al., 2019; Vaz et al., 2019; Kang et al., 2020; Krogh et al., 2022; Hofmeijer et al., 2023; Zhou et al., 2023; Chen et al., 2024; Jiang et al., 2024; Wang J. et al., 2024; Zeng et al., 2024; Wang et al., 2025; Jia et al., 2025). Of the seven studies on upper limb activity, only two (28.6%) reported that rTMS was effective in improving this outcome after stroke. These studies showed a low (SMD: −0.32; CI: −0.55 to −0.09; Zhang et al., 2017a) to moderate (SMD: −0.50; CI: −0.73 to −0.27) effect size, with low heterogeneity index (34.2%; p-value 0.07; Table 1; Zhang N. et al., 2024). The NNT varied from 6 to 13 (Supplementary Table 2). However, all studies that investigated the effects of rTMS in upper limb activity showed considerable variability in intervention protocols and lower sample sizes and a large CI (Table 3b), leading to an overall very low quality of evidence.

Of the 14 studies that investigated the rTMS effects on mobility, seven (50 %) studies (Li et al., 2018a; Ghayour-Najafabadi et al., 2019; Tung et al., 2019; Kang et al., 2020; Zhou et al., 2023; Wang J. et al., 2024; Wang et al., 2025) found that rTMS slightly and three (21.4%) potentially improved mobility outcomes (Figure 3; Vaz et al., 2019; Daoud et al., 2024; Zeng et al., 2024). These studies showed a low SMD: −0.29 (−0.52 to −0.05; Wang et al., 2025) to high (SMD: −0.97; CI: −1.28 to −0.66; Vaz et al., 2019) effect size, with low inconsistency indices (Table 1). The NNT varied from 4 to 13 (Supplementary Table 2). The study with higher effect size was classified as “low quality of evidence” due to substantial variation in rTMS protocols and heterogeneity in participant characteristics, particularly regarding time since stroke (Table 3b).

Finally, 15 (83.3%) of 18 studies reported that performance in ADL was potentially or slightly improved after rTMS in stroke survivors (Figure 3; Shen et al., 2017; Liu et al., 2019; Xiang et al., 2019; Ahmed et al., 2023; Chen X. et al., 2023; Chen Y. et al., 2023; Gao et al., 2023; Hofmeijer et al., 2023; Xi et al., 2023; Daoud et al., 2024; Ren et al., 2024; Zhu et al., 2024; Wang Y. et al., 2024; Xie et al., 2025; Wang et al., 2025). One study was classified as moderate quality of evidence (Xiang et al., 2019). Besides the high effect size (SMD: −0.82; IC: −1.05 to −0.59; NNT: 5) with low heterogeneity index (0%, p-value: 0.78), we downgrade one point in risk of bias, because this study was classified as “moderate” in AMSTAR classification (Table 3b). Three studies (30%) were classified as “low quality of evidence” (Chen Y. et al., 2023; Shen et al., 2017; Liu et al., 2019), besides they presented high effect sizes (Supplementary Table 2, Table 3b) and 14 (77.8%) studies were classified as “very low quality of evidence.”

3.6 Efficacy of tDCS for body structure/function

Figures 4, 5 summarize the clinical efficacy of tDCS across meta-analyses, mapped according to SMD, methodological quality (AMSTAR score), outcome domain (classified according to the ICF framework), sample size, stimulation protocol, and adverse event reporting. Figure 4 presents outcomes related to body structure/function, while Figure 5, discussed in Section 3.6, refers to activity.

Figure 4

Figure 4. Evidence map for the use of transcranial direct current stimulation (tDCS) on body structure and function. “Mixed protocols” indicates that various non-invasive brain stimulation (NIBS) protocols were included within the same meta-analysis.

Figure 5

Figure 5. Evidence map for the use of transcranial direct current stimulation (tDCS) on activity. “Mixed protocols” indicates that various non-invasive brain stimulation (NIBS) protocols were included within the same meta-analysis.

Fourteen meta-analyses, ranging from 4 studies (84 patients; Li et al., 2018b) to 42 studies (1596 patients; Tang et al., 2024), investigated the effect of tDCS on body structure and function. The outcome measures considered were: (1) FMA-UE, (2) FMA-LE, (3) MAS. Details on each measure are provided in Table 1.

Six of fourteen (42.9%) reported that tDCS was slightly or potentially effective in improving motor function after stroke (Figure 4; Li et al., 2018b; Sun et al., 2021; Van Hoornweder et al., 2021; Huang et al., 2022; Tang et al., 2024; Yu et al., 2025). These studies reported effect sizes ranging from low (SMD: −0.22; CI: −0.32 to −0.12; Tang et al., 2024) to high (SMD: −1.54; 95% CI: −2.78 to −0.29; Li et al., 2018b). The NNT varied from 3 to 17, with very serious inconsistency and imprecision issues, with higher heterogeneity indexes (I² > 40%; p-value < 0.01; Table 1). Thus, the overall quality of evidence for this outcome was deemed “very low” (Table 4a).

3.7 Efficacy of tDCS for stroke activity

Nineteen meta-analyses investigated the effect of tDCS on outcomes related to ICF domain of activity. Of the 19 studies, five (26.3%) examined upper limb activity (O'Brien et al., 2018; Elsner et al., 2020; Comino-Suárez et al., 2021; Reis et al., 2021; Yu et al., 2025), seven (36.8%) investigated mobility (Li et al., 2018b; Vaz et al., 2019; Elsner et al., 2020; Kang et al., 2020; Tien et al., 2020; Lima et al., 2023; Usman et al., 2024), and seven (33.3%) focused on ADL post-stroke (Tedesco Triccas et al., 2016; Elsner et al., 2020; Comino-Suárez et al., 2021; Ahmed et al., 2023; Zhang N. et al., 2024; Tang et al., 2024; Yu et al., 2025; Figure 5).

Three studies of five (60%) observed that tDCS was effective in improving upper limb activity. Reported effect sizes were low (SMD: −0.31; CI: −0.55 to −0.01 and SMD: −0.31; CI: −0.45 to −0.16; NNT: 12) or moderate (SMD: -0.59; CI: -0.89 to -0.30; NNT: 7), with low heterogeneity indices (O'Brien et al., 2018; Elsner et al., 2020; Yu et al., 2025) (Table 1). However, the studies exhibited considerable variability in the included protocols and the time since stroke onset, then we downgraded one point in inconsistency. One study did not present a meta-analysis graph (O'Brien et al., 2018), because of this, we downgraded two points for imprecision (for details, see the Table 4b).

Regarding mobility, three (Elsner et al., 2020; Tien et al., 2020; Lima et al., 2023) of seven studies (42.9%) reported slight improvements following tDCS (Figure 5). These studies showed a low effect size with low inconsistency indices. The NNT varied from 9 to 18. Overall, the studies were classified as “very low quality of evidence” because it presented high variability between tDCS protocols, included individuals with different times since stroke, presented a small sample size and larger CI intervals (Table 4b, Supplementary Table 2).

Finally, four of seven studies (57.1%) showed that the performance in ADL was slightly (Elsner et al., 2020; Tang et al., 2024) or potentially (Ahmed et al., 2023; Yu et al., 2025) increased following tDCS. The NNT varied from 5 to 13. The study with higher effect size was classified as “very low quality of evidence” because it was classified as “critically low” in AMSTAR, a high variability between tDCS protocols and included patients with different time since stroke (Table 4b).

We summarized the current evidence supporting motor function improvements with NIBS (rTMS/tDCS) within the principal domains of the ICF framework in Figure 6. This figure presents a visual synthesis of the motor functions most frequently reported as positively influenced by NIBS, accompanied by a rank-ordered list indicating the number and percentage of studies supporting each functional outcome.

Figure 6

Figure 6. A summary of the current evidence supporting the effects of repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS) within the ICF framework in post-stroke individuals.

3.8 Safety of NIBS for stroke

Table 1 also reports the adverse events reported in the rTMS and tDCS studies. 10 (25.6%) meta-analyses of rTMS (Shen et al., 2017; Zhang et al., 2017b; Liu et al., 2019; Tung et al., 2019; Xiang et al., 2019; Gao et al., 2023; Chen X. et al., 2023; Chen Y. et al., 2023; Wang J. et al., 2024; Xie et al., 2025) and three (14.3%) of tDCS report the occurrence of severe adverse effects after the stimulation (Comino-Suárez et al., 2021; Tedesco Triccas et al., 2016; Yu et al., 2025). For rTMS, commonly reported adverse effects include: headache, gastrointestinal reaction, tinnitus, feel weak, anxiety, nausea, tingling, dizziness, fatigue, drowsiness, neck pain, cast irritation, palpitation, and neurocardiogenic syncope. For tDCS, commonly reported adverse effects included: headache, dizziness, fatigue and tingling (Table 1). It is also important to highlight that some meta-analysis failed to report adverse effects in the results: thirteen for rTMS (37.1% of rTMS studies; Zhang et al., 2017a; Li et al., 2018a; van Lieshout et al., 2019; Krogh et al., 2022; Hofmeijer et al., 2023; Xi et al., 2023; Wang Y. et al., 2024; Zeng et al., 2024; Zhang N. et al., 2024; Barreto et al., 2025; Jia et al., 2025; Ma et al., 2025; Zhang et al., 2025), six for tDCS (37.5% of tDCS studies; Elsner et al., 2016; Reis et al., 2021; Sun et al., 2021; Van Hoornweder et al., 2021; Lima et al., 2023; Zhang J. J. et al., 2024), and four for rTMS and tDCS studies (80% of included meta-analyses; O'Brien et al., 2018; Vaz et al., 2019; Kang et al., 2020; Ahmed et al., 2023).

4 Discussion

This umbrella review is the first to synthesize and assess the quality of evidence from meta-analyses on NIBS in stroke rehabilitation, using the principal domains of ICF as a framework. Regarding the body structure/function domain, rTMS was more often associated with moderate to high effect sizes, particularly for general neurological function. In contrast, although some meta-analyses suggested that tDCS may have slight to potentially meaningful effects on motor function recovery, the certainty of this evidence was rated as very low due to serious concerns related to heterogeneity, imprecision, and variability in stimulation protocols. In the activity domain, both techniques showed modest effects, with rTMS demonstrating more favorable results for ADL than for mobility or upper limb activity. tDCS effects on activity-related outcomes were generally limited and supported by low to very low certainty of evidence across most outcomes. Furthermore, although no serious adverse events were reported across the meta-analyses, moderate adverse effects, including headache, fatigue, and occasional episodes of neurocardiogenic syncope, were documented. These findings indicate that while NIBS appears to have an acceptable safety profile, its tolerability may vary among individuals and should be carefully monitored in clinical applications.

When interpreting the magnitude of treatment effects observed in this umbrella review, it is important to consider thresholds for clinical relevance (Citrome, 2014). Although there is no universally accepted cutoff, we adopted a standardized mean difference (SMD) of 0.7 as a conservative benchmark for clinically meaningful effects, which is slightly above the conventional threshold for a moderate effect size (Rahlfs and Zimmermann, 2019; Zieliński, 2025). Effects at or above this level may reflect changes likely to translate into noticeable improvements in patient outcomes. However, it is important to recognize that the clinical significance of these effects can vary depending on the specific outcome assessed, the patient population, and the context of rehabilitation (Cuijpers et al., 2014). Therefore, while effect sizes below this threshold should be interpreted with caution, clinical decision-making should also integrate factors such as feasibility, patient preferences, and safety (Page, 2014). Indeed, we found more consistent clinically meaningful effects of rTMS in improving motor function, general neurologic function and performance in ADL. For tDCS, the body of evidence remain uncertain, with few studies presenting clinically meaningful effects just for motor function and performance in ADL.

4.1 Methodological quality of meta-analyses

The methodological quality of the included meta-analyses, as assessed by the AMSTAR tool, was predominantly moderate to high, with over 80% of the studies meeting key methodological criteria. These findings suggest increasing adherence to rigorous review practices and a growing methodological maturity in this research area. Only a small proportion were rated as low or critically low quality (14.6%), suggesting that some methodological inconsistencies still remain.

There were some limitations regarding the quality of evidence of the studies included in this Umbrella Review, such as the variability of protocols, which limited comparisons between studies, and the relatively small sample sizes that may compromise statistical robustness and reduce the generalizability of the results. Furthermore, the predominance of “low” or “very low” certainty ratings according to GRADE reflects imprecision and inconsistency in effect estimates, largely due to heterogeneity in intervention protocols, risk of bias, and variability in outcome measures and evaluation methods. These factors combined reduce confidence in the reliability of the findings and underscore the need for more rigorous, standardized randomized controlled trials and meta-analyses to strengthen the evidence base and improve the clinical applicability of NIBS in stroke rehabilitation.

The most frequent sources of methodological concern were the lack of justification for deviations from the original review protocols, incomplete or insufficiently reported search strategies, and the omission of funding information for the primary studies. These issues have important implications for the interpretation of findings. Unreported protocol deviations reduce transparency and increase the risk of selective reporting, which may introduce bias in the synthesis process. Inadequate search strategies can result in the exclusion of relevant studies, particularly negative trials, potentially inflating the estimated effects due to publication bias. Moreover, the failure to report funding sources of included studies limits the ability to assess conflicts of interest, which could compromise the neutrality of the evidence base. To address these issues, future reviews should aim to ensure compliance with all items outlined in the PRISMA 2020 guidelines, which are essential to enhance the credibility, transparency, and reproducibility of evidence syntheses in the field of NIBS.

4.2 Effects of rTMS on ICF domains in post-stroke rehabilitation

In our review, rTMS demonstrated the most consistent and clinically relevant effects within the ICF domain of body structure and function, particularly general neurological function. Nearly all studies evaluating this outcome reported positive effects of rTMS, with relatively low variability in effect magnitude, ranging from moderate (Shao et al., 2021) to high (Allida et al., 2020). Similarly, among the 26 meta-analyses that investigated motor function, more than half reported significant improvements in stroke recovery, with effect sizes also ranging from moderate to high.

Although the overall body of evidence supports a beneficial effect of rTMS in improving outcomes within the ICF domain of body structure and function, the findings related to motor function were more heterogeneous in terms of effect magnitude. This variability likely reflects multiple contributing factors, including differences in stimulation protocols (e.g., frequency, intensity, target site), patient characteristics (e.g., time since stroke, severity), methodological quality, and the number of studies synthesized within each meta-analysis. Meta-analyses with larger sample sizes, such as those by Chen Y. et al. (2023), Xi et al. (2023), and Hofmeijer et al. (2023), tended to report stronger and relevant effect sizes. This observation is consistent with methodological recommendations emphasizing the importance of adequately powered studies to reduce the risk of bias and enhance the precision of effect estimates (Ioannidis, 2005).

It is important to note that, compared with motor function outcomes, the effects of rTMS on general neurological function (e.g., as measured by NIHSS) were more consistent across studies, despite variations in stimulation protocols. One possible explanation for this lower variability is that such outcomes are broader in scope, capturing diffuse neurological changes that may occur across multiple functional systems. In contrast, motor function outcomes, particularly those assessing specific limb performance with tools such as the FMA, are more narrowly focused and may be more susceptible to individual variability, such as lesion location, stroke severity, or rehabilitation context. This discrepancy highlights the importance of carefully selecting and clearly defining outcome measures in neuromodulation trials. Notably, the methodological quality of the studies evaluating general neurological function was also higher (e.g., Allida et al., 2020; Liu et al., 2019; Shen et al., 2017), which may have contributed to more consistent results. Methodological rigor is known to influence the reliability of meta-analytic findings; systematic reviews with high AMSTAR-2 scores are more likely to produce valid and unbiased estimates (Shea et al., 2017).

Among the meta-analyses assessing activity outcomes, the effects of rTMS were generally less consistent and less robust than those observed for body structure and function, except for ADL. Sixteen out of eighteen studies evaluating ADL reported slight to potentially meaningful improvements following rTMS. Among them, only the study by Xiang et al. (2019) achieved moderate certainty of evidence and reported a large effect size with low heterogeneity, likely due to its use of subgroup analyses based on stroke population characteristics and the application of optimized stimulation parameters. In contrast, the evidence for mobility was less consistent. Although the majority of studies reported positive effects, effect sizes varied widely from small to large and all were rated as low or very low certainty of evidence, primarily due to heterogeneity in stimulation protocols and participant characteristics. The weakest evidence was observed for upper limb activity: only two out of seven studies demonstrated a statistically significant effect, and all were rated as very low certainty, largely also reflecting protocol inconsistencies.

While most meta-analyses evaluating rTMS for post-stroke rehabilitation report slight to potentially meaningful effects, especially for outcomes such as general neurological function and ADL, differences in stimulation parameters, patient characteristics, and outcome definitions likely obscure the consistency of the evidence and contribute to the predominance of low or very low certainty ratings in GRADE assessments. In many meta-analyses, the inclusion of trials with markedly divergent methodologies has reduced the consistency and precision of the pooled estimates, ultimately lowering the overall quality of the evidence.

At the same time, the NIBS field is moving toward increasingly personalized rTMS interventions, with growing efforts to tailor stimulation protocols based on lesion location, functional reserve, neurophysiological markers, and time since stroke (Hildesheim et al., 2022). While this individualized approach holds promise for improving patient-level outcomes, it also introduces new layers of heterogeneity that may further complicate evidence synthesis. As protocols become more specific to individual profiles, future meta-analyses may face greater challenges in aggregating results, potentially reinforcing the trend of low certainty of evidence unless new strategies are developed to standardize personalization frameworks without compromising clinical relevance. Establishing clinical guidelines that balance inter-individual variability with methodological rigor will be crucial.

When comparing the domains of body structure/function and activity, rTMS appears to have a stronger and more consistent effect on body structure and function outcomes than on activity outcomes. As discussed earlier, most meta-analyses evaluating motor and general neurological function reported moderate to high effect sizes with relatively low variability. In contrast, outcomes related to activity, particularly those assessing mobility and upper limb use, demonstrated greater heterogeneity and lower certainty of evidence. One possible explanation is that improvements in impairment-level outcomes (e.g., motor function) may not directly translate into higher-level functional activities, especially in the absence of structured, context-specific rehabilitation. Functional outcomes such as mobility and upper limb use often require meaningful behavior change, including the integration of newly recovered abilities into daily routines. Moreover, the relatively short duration of most NIBS protocols—typically limited to 10 to 20 sessions—may be insufficient to promote the sustained engagement and task-specific motor learning needed to drive long-term functional gains in real-world settings.

4.3 Effects of tDCS on ICF domains in post-stroke rehabilitation

A substantial number of meta-analyses have examined the effects of tDCS on motor recovery and functional performance after stroke. However, findings across studies remain heterogeneous, particularly for outcomes related to body structure and function. While some reviews reported moderate to large effect sizes for motor improvements (Li et al., 2018b; Sun et al., 2021; Van Hoornweder et al., 2021; Huang et al., 2022), the lack of consistency across meta-analyses and the predominance of low-certainty evidence hinder the formulation of clear clinical recommendations. In contrast, outcomes related to activity, especially ADL, more frequently showed evidence of benefit (O'Brien et al., 2018; Elsner et al., 2020; Tien et al., 2020; Ahmed et al., 2023; Lima et al., 2023; Tang et al., 2024), though with smaller effect sizes. These findings were also characterized by substantial methodological limitations, such as such as the low quality of several meta-analyses, inconsistency in methods of the included studies in each meta-analyses and imprecise results.

Meta-analyses with larger sample sizes (Huang et al., 2022; Van Hoornweder et al., 2021; Tang et al., 2024; Elsner et al., 2020) tended to report more robust and stable effect estimates, underscoring the pivotal role of sample size in the reliability of pooled outcomes. For instance, while Huang et al. (2022) and Van Hoornweder et al. (2021) reported large standardized mean differences, these were accompanied by very high inconsistency indices (I² > 60%). This reinforces a well-recognized concern in complex interventions such as NIBS: small, underpowered studies are more prone to random error and effect size inflation (Button et al., 2013; Mitra et al., 2019; Andrade, 2020). The precision and reliability of effect estimates can be significantly improved by increasing sample size and maintaining methodological rigor.

Methodologically, the body of evidence on tDCS appears less robust than that on rTMS. Most reviews were rated as low or critically low quality, according to the AMSTAR-2 tool, and all but one were classified as providing low or very low certainty of evidence by GRADE. These methodological limitations, such as lack of protocol registration, absence of publication bias assessment, and inconsistencies in risk of bias evaluation, undermine the reliability of the conclusions and underscore the need for higher-quality evidence syntheses in this area (Shea et al., 2017).

Taken together, the findings suggest that although tDCS holds promise for improving motor recovery and functional performance in individuals with stroke, current evidence remains limited by small sample sizes, heterogeneous protocols, and methodological weaknesses. Future research should address these gaps through well-designed, adequately powered trials and rigorous evidence syntheses.

Importantly, the clinical heterogeneity observed across meta-analyses likely reflects, at least in part, the individualized nature of tDCS application. As a neuromodulation technique, tDCS is often tailored to a patient's specific clinical characteristics, such as stroke chronicity, lesion site, or level of impairment (Simonetta-Moreau, 2014; Baltar et al., 2020), resulting in a degree of protocol variability that is not only expected but also necessary to accommodate diverse rehabilitation needs. While this variability complicates direct comparisons and evidence synthesis, it also underscores the importance of developing analytic strategies capable of capturing clinically relevant heterogeneity, rather than penalizing it as a methodological weakness.

Although this overview selected the most representative meta-analyses for each outcome, many incorporated subgroup analyses within their synthesis. While this strategy enhances generalizability, it may also have obscure clinically meaningful effects linked to more individualized stimulation parameters. By aggregating heterogeneous data without stratification, the resulting estimates tend to show greater variability, which may lead to downgraded certainty of evidence and attenuate effect sizes. The absence of subgroup analyses, despite their potential to identify more effective, tailored interventions, may therefore contribute to underestimating the therapeutic potential of tDCS in specific patient profiles. Consequently, the true clinical impact of tDCS may have been partially diminished by fragmented or overly narrow analytical approaches, reinforcing the need for meta-analyses that balance granularity with statistical power.

4.4 Safety of NIBS for the stroke treatment

The reporting of adverse effects across the included meta-analyses was limited and inconsistent, restricting the ability to comprehensively assess the safety of NIBS after stroke. Although some studies described mild to moderate side effects—such as headache, dizziness, fatigue, and tingling—severe adverse events were reported in only a minority of meta-analyses (25.6% for rTMS and 14.3% for tDCS). The heterogeneity in types and frequencies of adverse effects likely reflects both real differences across protocols and populations, as well as variability in how primary studies monitor and report safety outcomes. Notably, more than one-third of the meta-analyses failed to mention adverse events at all. This underreporting represents a significant methodological limitation in the NIBS literature and underscores the urgent need for standardized reporting of safety data in future trials and evidence syntheses. Without such transparency, the clinical interpretation of risk–benefit ratios remains incomplete.

4.5 Limitations and future perspectives

As an umbrella review, this study plays an important role in promoting broader recognition of NIBS and informing professionals about its potential clinical benefits. However, few limitations must be acknowledged. First, the literature search was conducted exclusively in the MEDLINE (PubMed) database. Although PubMed is a widely recognized and comprehensive source for health-related research, restricting the search to a single database may have limited the retrieval of additional relevant meta-analyses. Additionally, although the search strategy was validated by experts in scientific methodology and NIBS, we did not include a medical librarian in the development of the search terms, which might have further optimized the process. Future updates should consider incorporating databases such as EMBASE, Scopus, and the Cochrane Library to enhance comprehensiveness and reduce publication bias.

Second, due to substantial heterogeneity in outcome measures and reporting, we were unable to provide a clear and exhaustive analysis of outcomes stratified by specific ICF domains and subdomains. This inconsistency—together with frequent overlap across domains—limited our ability to determine whether outcomes referred to walking, transfers, or other specific aspects of mobility. These limitations reflect variability in outcome reporting in the primary studies and meta-analyses. For example, it was not consistently possible to distinguish whether mobility-related outcomes referred specifically to walking, transfers, bed mobility, or community ambulation. Although we acknowledge that this level of detail would enhance the clinical applicability of the findings, the limitation stems from inconsistencies in outcome reporting within the primary studies and meta-analyses synthesized.

This methodological variability also limited the ability to determine which specific configurations might be associated with greater therapeutic efficacy. Similarly, although clinical factors such as lesion location, time since stroke, and lesion extent are known to influence individual responsiveness to NIBS, the available evidence did not allow for a more granular analysis of these variables. Additionally, identifying predictors of treatment response—distinguishing responders from non-responders—would require access to individual participant data or consistent subgroup analyses, which were rarely available across the reviews.

NIBS has evolved significantly in recent years, becoming an increasingly central intervention in post-stroke rehabilitation. The principle of personalization is fundamental to this approach, as it allows protocols to be adapted based on individual clinical characteristics, such as stroke severity, lesion location and patient functional profile (Coêlho et al., 2021). Personalized stimulation involves tailoring parameters, protocols, and patient selection criteria, optimizing treatment effectiveness and improve outcomes in an individualized manner (Kesselheim et al., 2023; Wessel et al., 2024). For this reason, techniques such as neuromodulation may yield suboptimal results when applied with “one size fits all” treatment, the lack of personalization can limit treatment efficacy (Ovadia-Caro et al., 2019). Our umbrella review was not designed to investigate distinctions between specific stimulation protocols or patient characteristics. Future reviews incorporating subgroup analyses should aim to identify stimulation protocols associated with greater therapeutic efficacy, as well as the investigation of whether clinical factors such as lesion location, time since stroke, stimulation dose, and lesion extent can predict responsiveness to NIBS. In addition, more studies with larger samples and long-term follow-up is needed to assess the durability of NIBS effects in post-stroke recovery.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author.

Author contributions

BR: Investigation, Data curation, Writing – original draft, Writing – review & editing, Methodology. LS: Investigation, Methodology, Writing – original draft, Data curation, Writing – review & editing. PL: Investigation, Data curation, Writing – review & editing. MS: Data curation, Writing – original draft. DM: Investigation, Data curation, Writing – original draft. RB: Formal analysis, Writing – review & editing, Writing – original draft, Investigation. AB: Writing – review & editing, Investigation, Methodology, Writing – original draft. RD: Writing – review & editing, Writing – original draft, Validation, Methodology. GB: Data curation, Writing – review & editing, Writing – original draft. RA: Writing – review & editing, Writing – original draft, Data curation, Methodology, Investigation. KN-S: Writing – review & editing, Investigation, Writing – original draft, Data curation. AFB: Investigation, Writing – original draft, Data curation, Writing – review & editing. DP: Methodology, Validation, Investigation, Writing – review & editing, Conceptualization, Supervision. KM-S: Writing – original draft, Writing – review & editing, Investigation, Funding acquisition, Visualization, Methodology, Validation, Conceptualization, Supervision.

Funding

The author(s) declare that financial support was received for the research and/or publication of this article. AFB was supported by CNPq/Brazil (Grant 314149/2018-0), KM-S was supported by CNPq/Brazil (Grant 31 1224/2019-9), KS was supported by FUNADESP/Brazil (Grant 60-123/2022), and LS was supported by CNPq/Brazil (Grant 371559/2024-3).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Generative AI statement

The author(s) declare that no Gen AI was used in the creation of this manuscript.

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Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnins.2025.1633986/full#supplementary-material

References

Ahmed, I., Mustafaoglu, R., Benkhalifa, N., and Yakhoub, Y. H. (2023). Does noninvasive brain stimulation combined with other therapies improve upper extremity motor impairment, functional performance, and participation in activities of daily living after stroke? A systematic review and meta-analysis of randomized controlled trial. Top. Stroke Rehabil. 30, 213–234. doi: 10.1080/10749357.2022.2026278

PubMed Abstract | Crossref Full Text | Google Scholar

Ahmed, I., Yeldan, I., and Mustafaoglu, R. (2022). The adjunct of electric neurostimulation to rehabilitation approaches in upper limb stroke rehabilitation: a systematic review with network meta-analysis of randomized controlled trials. Neuromodulation 25, 1197–1214. doi: 10.1016/j.neurom.2022.01.005

PubMed Abstract | Crossref Full Text | Google Scholar

Allida, S., Cox, K. L., Hsieh, C. F., Lang, H., House, A., and Hackett, M. L. (2020). Pharmacological, psychological, and non-invasive brain stimulation interventions for treating depression after stroke. Cochrane Database Syst. Rev. 1:CD003437. doi: 10.1002/14651858.CD003437.pub4

PubMed Abstract | Crossref Full Text | Google Scholar

Andrade, C. (2020). Sample size and its importance in research. Indian J. Psychol. Med. 42, 102–103. doi: 10.4103/IJPSYM.IJPSYM_504_19

PubMed Abstract | Crossref Full Text | Google Scholar

Aromataris, E., Fernandez, R., Godfrey, C. M., Holly, C., Khalil, H., and Tungpunkom, P. (2015). Summarizing systematic reviews: methodological development, conduct and reporting of an umbrella review approach. Int. J. Evid. Based Healthc. 13, 132–140. doi: 10.1097/XEB.0000000000000055

PubMed Abstract | Crossref Full Text | Google Scholar

Baltar, A., Piscitelli, D., Marques, D., Shirahige, L., and Monte-Silva, K. (2020). Baseline motor impairment predicts transcranial direct current stimulation combined with physical therapy-induced improvement in individuals with chronic stroke. Neural Plast. 2020:8859394. doi: 10.1155/2020/8859394

PubMed Abstract | Crossref Full Text | Google Scholar

Barreto, G., Sánchez, P., Dias, R., Baltar, A., Shirahige, L., Fragoso de Andrade, R., et al. (2025). The impact of the number of sessions and stimulation parameters on repetitive transcranial magnetic stimulation efficacy for post-stroke upper extremity recovery: a systematic review and meta-analysis. Clin. Rehabil. 39, 707–727. doi: 10.1177/02692155251328945

PubMed Abstract | Crossref Full Text | Google Scholar

Brunoni, A. R., Sampaio-Junior, B., Moffa, A. H., Aparício, L. V., Gordon, P., Klein, I., et al. (2019). Noninvasive brain stimulation in psychiatric disorders: a primer. Rev. Brasil. Psiquiatria 41, 70–81. doi: 10.1590/1516-4446-2017-0018

PubMed Abstract | Crossref Full Text | Google Scholar

Button, K. S., Ioannidis, J. P., Mokrysz, C., Nosek, B. A., Flint, J., Robinson, E. S., et al. (2013). Power failure: why small sample size undermines the reliability of neuroscience. Nature reviews. Neuroscience 14, 365–376. doi: 10.1038/nrn3475

PubMed Abstract | Crossref Full Text | Google Scholar

Chen, K., Sun, M., and Zhuang, H. (2024). Effect of theta burst stimulation on lower extremity motor function improvement and balance recovery in patients with stroke: a systematic review and meta-analysis of randomized controlled trials. Medicine 103:e40098. doi: 10.1097/MD.0000000000040098

PubMed Abstract | Crossref Full Text | Google Scholar

Chen, X., Liu, F., Lyu, Z., Xiu, H., Hou, Y., and Tu, S. (2023). High-frequency repetitive transcranial magnetic stimulation (HF-rTMS) impacts activities of daily living of patients with post-stroke cognitive impairment: a systematic review and meta-analysis. Neurol. Sci. 44, 2699–2713. doi: 10.1007/s10072-023-06779-9

PubMed Abstract | Crossref Full Text | Google Scholar

Chen, Y., Yang, L., Li, X., Tang, L., Pi, Y., and Bai, D. (2023). Non-invasive brain stimulation for limb motor function and daily living activity improvement in acute stroke: a meta-analysis. J. Stroke Cerebrovasc. Dis. 32:106982. doi: 10.1016/j.jstrokecerebrovasdis.2023.106982

PubMed Abstract | Crossref Full Text | Google Scholar

Citrome, L. (2014). Quantifying clinical relevance. Innov. Clin. Neurosci. 11, 26–30.

Google Scholar

Coêlho, M. L. S., Cabral, P. M., Morello, L. Y. N., Rêgo, G. G. d., and Boggio, P. S. (2021). “Estimulações cerebrais não invasivas: as aplicações de Estimulação Transcraniana por Corrente Contínua (ETCC) e Estimulação Magnética Transcraniana (EMT) na aprendizagem e na clínica,” in Tecnologias aplicadas em Educação e Saúde, ed A. G. D. Corrêa (São Paulo: Memnon Edições Científicas), 341–362. doi: 10.29327/558730.1-21

Crossref Full Text | Google Scholar

Comino-Suárez, N., Moreno, J. C., Gómez-Soriano, J., Megía-García, Á., Serrano-Muñoz, D., Taylor, J., et al. (2021). Transcranial direct current stimulation combined with robotic therapy for upper and lower limb function after stroke: a systematic review and meta-analysis of randomized control trials. J. Neuroeng. Rehabil. 18:148. doi: 10.1186/s12984-021-00941-0

PubMed Abstract | Crossref Full Text | Google Scholar

Cuijpers, P., Turner, E. H., Koole, S. L., van Dijke, A., and Smit, F. (2014). What is the threshold for a clinically relevant effect? The case of major depressive disorders. Depress. Anxiety 31, 374–378. doi: 10.1002/da.22249

PubMed Abstract | Crossref Full Text | Google Scholar

Daoud, A., Elsayed, M., Alnajjar, A. Z., Krayim, A., AbdelMeseh, M., Alsalloum, T., et al. (2024). Efficacy of intermittent theta burst stimulation (iTBS) on post-stroke cognitive impairment (PSCI): a systematic review and meta-analysis. Neurol. Sci. 45, 2107–2118. doi: 10.1007/s10072-023-07267-w

PubMed Abstract | Crossref Full Text | Google Scholar

Elsner, B., Kugler, J., Pohl, M., and Mehrholz, J. (2016). Transcranial direct current stimulation for improving spasticity after stroke: a systematic review with meta-analysis. J. Rehabil. Med. 48, 565–570. doi: 10.2340/16501977-2097

PubMed Abstract | Crossref Full Text | Google Scholar

Elsner, B., Kugler, J., Pohl, M., and Mehrholz, J. (2020). Transcranial direct current stimulation (tDCS) for improving activities of daily living, and physical and cognitive functioning, in people after stroke. Cochrane Database Syst. Rev. 11:CD009645. doi: 10.1002/14651858.CD009645.pub4

PubMed Abstract | Crossref Full Text | Google Scholar

Furukawa, T. A., and Leucht, S. (2011). How to obtain NNT from Cohen's d: comparison of two methods. PloS one 6:e19070. doi: 10.1371/journal.pone.0019070

PubMed Abstract | Crossref Full Text | Google Scholar

Gallardo-Gómez, D., Richardson, R., and Dwan, K. (2024). Standardized mean differences in meta-analysis: a tutorial. Cochrane Evid. Synth. Methods 2:e12047. doi: 10.1002/cesm.12047

PubMed Abstract | Crossref Full Text | Google Scholar

Gao, Y., Qiu, Y., Yang, Q., Tang, S., Gong, J., Fan, H., et al. (2023). Repetitive transcranial magnetic stimulation combined with cognitive training for cognitive function and activities of daily living in patients with post-stroke cognitive impairment: a systematic review and meta-analysis. Ageing Res. Rev. 87:101919. doi: 10.1016/j.arr.2023.101919

PubMed Abstract | Crossref Full Text | Google Scholar

Gates, M., Gates, A., Pieper, D., Fernandes, R. M., Tricco, A. C., Moher, D., et al. (2022). Reporting guideline for overviews of reviews of healthcare interventions: development of the PRIOR statement. BMJ 378:e070849. doi: 10.1136/bmj-2022-070849

PubMed Abstract | Crossref Full Text | Google Scholar

Ghayour-Najafabadi, M., Memari, A. H., Hosseini, L., Shariat, A., and Cleland, J. A. (2019). Repetitive transcranial magnetic stimulation for the treatment of lower limb dysfunction in patients poststroke: a systematic review with meta-analysis. J. Stroke Cerebrovasc. Dis. 28:104412. doi: 10.1016/j.jstrokecerebrovasdis.2019.104412

PubMed Abstract | Crossref Full Text | Google Scholar

Graef, P., Dadalt, M. L. R., Rodrigués, D. A. M. D. S., Stein, C., and Pagnussat, A. S. (2016). Transcranial magnetic stimulation combined with upper-limb training for improving function after stroke: a systematic review and meta-analysis. J. Neurol. Sci. 369, 149–158. doi: 10.1016/j.jns.2016.08.016

PubMed Abstract | Crossref Full Text | Google Scholar

Higgins, J., and Welch, V. (n.d.). Cochrane Handbook for Systematic Reviews of Interventions. Available online at: https://training.cochrane.org/handbook (Accessed April 17, 2025).

Google Scholar

Higgins, J. P. T., Thomas, J., Chandler, J., Cumpston, M., Li, T., and Page, M. J., (eds.). (2024). Cochrane Handbook for Systematic Reviews of Interventions Version 6.5 (updated August 2024). Cochrane. Available online at: www.cochrane.org/handbook

Google Scholar

Hildesheim, F. E., Silver, A. N., Dominguez-Vargas, A. U., Andrushko, J. W., Edwards, J. D., Dancause, N., et al. (2022). Predicting individual treatment response to rTMS for motor recovery after stroke: a review and the CanStim perspective. Front. Rehabil. Sci. 3:795335. doi: 10.3389/fresc.2022.795335

PubMed Abstract | Crossref Full Text | Google Scholar

Hofmeijer, J., Ham, F., and Kwakkel, G. (2023). Evidence of rTMS for motor or cognitive stroke recovery: hype or hope. Stroke 54, 2500–2511. doi: 10.1161/STROKEAHA.123.043159

PubMed Abstract | Crossref Full Text | Google Scholar

Huang, J., Qu, Y., Liu, L., Zhao, K., and Zhao, Z. (2022). Efficacy and safety of transcranial direct current stimulation for post-stroke spasticity: a meta-analysis of randomised controlled trials. Clin. Rehabil. 36, 158–171. doi: 10.1177/02692155211038097

PubMed Abstract | Crossref Full Text | Google Scholar

Ioannidis, J. P. A. (2005). Why most published research findings are false. PLoS Med. 2:e124. doi: 10.1371/journal.pmed.0020124

PubMed Abstract | Crossref Full Text | Google Scholar

Jia, D. M., Li, X., Zhang, B. C., Zhang, B. R., Zhang, Q. J., Liu, M. W., et al. (2025). Therapeutic efficacy of repetitive transcranial magnetic stimulation on gait and limb balance function in patients with lower limb dysfunction post-cerebral infarction: a systematic review and meta-analysis. BMC Neurol. 25, 1–18. doi: 10.1186/s12883-025-04112-9

PubMed Abstract | Crossref Full Text | Google Scholar

Jiang, T., Wei, X., Wang, M., Xu, J., Xia, N., and Lu, M. (2024). Theta burst stimulation: what role does it play in stroke rehabilitation? A systematic review of the existing evidence. BMC Neurol. 24, 1–23. doi: 10.1186/s12883-023-03492-0

PubMed Abstract | Crossref Full Text | Google Scholar

Kang, N., Lee, R. D., Lee, J. H., and Hwang, M. H. (2020). Functional balance and postural control improvements in patients with stroke after noninvasive brain stimulation: a meta-analysis. Arch. Phys. Med. Rehabil. 101, 141–153. doi: 10.1016/j.apmr.2019.09.003

PubMed Abstract | Crossref Full Text | Google Scholar

Kang, N., Weingart, A., and Cauraugh, J. H. (2018). Transcranial direct current stimulation and suppression of contralesional primary motor cortex post-stroke: a systematic review and meta-analysis. Brain Inj. 32, 1063–1070. doi: 10.1080/02699052.2018.1481526

PubMed Abstract | Crossref Full Text | Google Scholar

Kesselheim, J., Takemi, M., Christiansen, L., Karabanov, A. N., and Siebner, H. R. (2023). Multipulse transcranial magnetic stimulation of human motor cortex produces short-latency corticomotor facilitation via two distinct mechanisms. J. Neurophysiol. 129, 410–420. doi: 10.1152/jn.00263.2022

PubMed Abstract | Crossref Full Text | Google Scholar

Kim, S., and Park, H. (2024). Update on non-invasive brain stimulation on stroke motor impairment: a narrative review. Brain Neurorehabil. 17, 1–7. doi: 10.12786/bn.2024.17.e5

PubMed Abstract | Crossref Full Text | Google Scholar

Krogh, S., Jønsson, A. B., Aagaard, P., and Kasch, H. (2022). Efficacy of repetitive transcranial magnetic stimulation for improving lower limb function in individuals with neurological disorders: a systematic review and meta-analysis of randomized Sham-controlled trials. J. Rehabil. Med. 54:jrm00256. doi: 10.2340/jrm.v53.1097

PubMed Abstract | Crossref Full Text | Google Scholar

Leonardi, M., and Fheodoroff, K. (2021). “Goal setting with ICF (international classification of functioning, disability and health) and multidisciplinary team approach in stroke rehabilitation,” in Clinical Pathways in Stroke Rehabilitation: Evidence-based Clinical Practice Recommendations, ed. T. Platz (Cham: Springer), 35–56. doi: 10.1007/978-3-030-58505-1_3

PubMed Abstract | Crossref Full Text | Google Scholar

Li, Y., Fan, J., Yang, J., He, C., and Li, S. (2018a). Effects of repetitive transcranial magnetic stimulation on walking and balance function after stroke: a systematic review and meta-analysis. Am. J. Phys. Med. Rehabil. 97, 773–781. doi: 10.1097/PHM.0000000000000948

PubMed Abstract | Crossref Full Text | Google Scholar

Li, Y., Fan, J., Yang, J., He, C., and Li, S. (2018b). Effects of transcranial direct current stimulation on walking ability after stroke: a systematic review and meta-analysis. Restor. Neurol. Neurosci. 36, 59–71. doi: 10.3233/RNN-170770

PubMed Abstract | Crossref Full Text | Google Scholar

Liew, S. L., Santarnecchi, E., Buch, E. R., and Cohen, L. G. (2014). Non-invasive brain stimulation in neurorehabilitation: local and distant effects for motor recovery. Front. Hum. Neurosci. 8:378. doi: 10.3389/fnhum.2014.00378

PubMed Abstract | Crossref Full Text | Google Scholar

Lima, E., de Souza Neto, J. M. R., and Andrade, S. M. (2023). Effects of transcranial direct current stimulation on lower limb function, balance and quality of life after stroke: a systematic review and meta-analysis. Neurol. Res. 45, 843–853. doi: 10.1080/01616412.2023.2211457

PubMed Abstract | Crossref Full Text | Google Scholar

Lima, E. O., Silva, L. M., Melo, A. L. V., D'arruda, J. V. T., Alexandre de Albuquerque, M., Ramos de Souza Neto, J. M., et al. (2024). Transcranial direct current stimulation and brain-computer interfaces for improving post-stroke recovery: a systematic review and meta-analysis. Clin. Rehabil. 38, 3–14. doi: 10.1177/02692155231200086

PubMed Abstract | Crossref Full Text | Google Scholar

Liu, C., Wang, M., Liang, X., Xue, J., and Zhang, G. (2019). Efficacy and safety of high-frequency repetitive transcranial magnetic stimulation for poststroke depression: a systematic review and meta-analysis. Arch. Phys. Med. Rehabil. 100, 1964–1975. doi: 10.1016/j.apmr.2019.03.012

PubMed Abstract | Crossref Full Text | Google Scholar

Liu, H. X., Hu, D. H., and Yin, H. Q. (2020). Umbrella review - a new method related to evidence-based medical analysis. Zhonghua Liu Xing Bing Xue Za Zhi 41, 261–266. doi: 10.3760/cma.j.issn.0254-6450.2020.02.021

PubMed Abstract | Crossref Full Text | Google Scholar

Ma, Z., Pan, H., Bi, R., Li, Z., Lu, W., and Wan, P. (2025). Systematic review of repetitive transcranial magnetic stimulation for post-stroke hemiplegic shoulder pain. Neurol. Sci. 46, 2007–2017. doi: 10.1007/s10072-024-07961-3

PubMed Abstract | Crossref Full Text | Google Scholar

Mahmoud, W., Baur, D., Zrenner, B., Brancaccio, A., Belardinelli, P., Ramos-Murguialday, A., et al. (2024). Brain state-dependent repetitive transcranial magnetic stimulation for motor stroke rehabilitation: a proof of concept randomized controlled trial. Front. Neurol. 15:1427198. doi: 10.3389/fneur.2024.1427198

PubMed Abstract | Crossref Full Text | Google Scholar

McIntyre, A., Mirkowski, M., Thompson, S., Burhan, A. M., Miller, T., and Teasell, R. (2018). A systematic review and meta-analysis on the use of repetitive transcranial magnetic stimulation for spasticity poststroke. PM&R 10, 293–302. doi: 10.1016/j.pmrj.2017.10.001

PubMed Abstract | Crossref Full Text | Google Scholar

Mitra, S., Mehta, U. M., Binukumar, B., Venkatasubramanian, G., and Thirthalli, J. (2019). Statistical power estimation in non-invasive brain stimulation studies and its clinical implications: an exploratory study of the meta-analyses. Asian J. Psychiatr. 44, 29–34. doi: 10.1016/j.ajp.2019.07.006

PubMed Abstract | Crossref Full Text | Google Scholar

O'Brien, A. T., Bertolucci, F., Torrealba-Acosta, G., Huerta, R., Fregni, F., and Thibaut, A. (2018). Non-invasive brain stimulation for fine motor improvement after stroke: a meta-analysis. Euro. J. Neurol. 25, 1017–1026. doi: 10.1111/ene.13643

PubMed Abstract | Crossref Full Text | Google Scholar

Ovadia-Caro, S., Khalil, A. A., Sehm, B., Villringer, A., Nikulin, V. V., and Nazarova, M. (2019). Predicting the response to non-invasive brain stimulation in stroke. Front. Neurol. 10:302. doi: 10.3389/fneur.2019.00302

PubMed Abstract | Crossref Full Text | Google Scholar

Page, P. (2014). Beyond statistical significance: clinical interpretation of rehabilitation research literature. Int. J. Sports Phys. Ther. 9, 726–736.

Google Scholar

Qi, L., Wang, S., Li, X., Yu, Y., Wang, W., Li, Q., et al. (2024). Non-invasive brain stimulation in the treatment of generalized anxiety disorder: a systematic review and meta-analysis. J. Psychiatr. Res. 178, 378–387. doi: 10.1016/j.jpsychires.2024.07.046

PubMed Abstract | Crossref Full Text | Google Scholar

Rahlfs, V., and Zimmermann, H. (2019). Effect size measures and their benchmark values for quantifying benefit or risk of medicinal products. Biom. J. 61, 973–982. doi: 10.1002/bimj.201800107

PubMed Abstract | Crossref Full Text | Google Scholar

Reis, S. B., Bernardo, W. M., Oshiro, C. A., Krebs, H. I., and Conforto, A. B. (2021). Effects of robotic therapy associated with noninvasive brain stimulation on upper-limb rehabilitation after stroke: systematic review and meta-analysis of randomized clinical trials. Neurorehabil. Neural Repair 35, 256–266. doi: 10.1177/1545968321989353

PubMed Abstract | Crossref Full Text | Google Scholar

Ren, M., Xu, J., Wang, W., Shen, L., Wang, C., Liu, H., et al. (2024). Effect of dual-site non-invasive brain stimulation on upper-limb function after stroke: a systematic review and meta-analysis. Brain Behav. 14:e70145. doi: 10.1002/brb3.70145

PubMed Abstract | Crossref Full Text | Google Scholar

Salter, K., Campbell, N., Richardson, M., Mehta, S., Jutai, J., Zettler, L., et al. (2019). “Outcome measures in stroke rehabilitation,” in Evidence-Based Review of Stroke Rehabilitation, 16th Edn., ed. R. Teasell (London, ON: Sockit Solutions). Available online at: http://www.ebrsr.com/

Google Scholar

Shao, D., Zhao, Z. N., Zhang, Y. Q., Zhou, X. Y., Zhao, L. B., Dong, M., et al. (2021). Efficacy of repetitive transcranial magnetic stimulation for post-stroke depression: a systematic review and meta-analysis of randomized clinical trials. Brazil. J. Med. Biol. Res. 54:e10010. doi: 10.1590/1414-431x202010010

PubMed Abstract | Crossref Full Text | Google Scholar

Shea, B. J., Reeves, B. C., Wells, G., Thuku, M., Hamel, C., Moran, J., et al. (2017). AMSTAR 2: a critical appraisal tool for systematic reviews that include randomised or non-randomised studies of healthcare interventions, or both. BMJ 358:J4008. doi: 10.1136/bmj.j4008

PubMed Abstract | Crossref Full Text | Google Scholar

Shen, Q., Hu, M., Feng, W., Li, K., and Wang, W. (2022). Narrative review of noninvasive brain stimulation in stroke rehabilitation. Med. Sci. Monit. 28:e938298. doi: 10.12659/MSM.938298

PubMed Abstract | Crossref Full Text | Google Scholar

Shen, X., Liu, M., Cheng, Y., Jia, C., Pan, X., Gou, Q., et al. (2017). Repetitive transcranial magnetic stimulation for the treatment of post-stroke depression: a systematic review and meta-analysis of randomized controlled clinical trials. J. Affect. Disord. 211, 65–74. doi: 10.1016/j.jad.2016.12.058

PubMed Abstract | Crossref Full Text | Google Scholar

Shirahige, L., Baptista, A., Sá, K. N., Baltar, A., Marques, D., Carneiro, M. S., et al. (2022). Non-invasive brain stimulation for the treatment of neurological and psychiatric disorders and for improving physical performance: protocol of umbrella reviews: protocolo de revisões guarda-chuva. Brain Imaging Stimul. 1:e4400. doi: 10.17267/2965-3738bis.2022.e4400

Crossref Full Text | Google Scholar

Simonetta-Moreau, M. (2014). Non-invasive brain stimulation (NIBS) and motor recovery after stroke. Ann. Phys. Rehabil. Med. 57, 530–542. doi: 10.1016/j.rehab.2014.08.003

PubMed Abstract | Crossref Full Text | Google Scholar

Stucki, G., Cieza, A., and Melvin, J. (2007). The International Classification of Functioning, Disability and Health (ICF): a unifying model for the conceptual description of the rehabilitation strategy. J. Rehabil. Med. 39, 279–285. doi: 10.2340/16501977-0041

PubMed Abstract | Crossref Full Text | Google Scholar

Subramanian, S. K., and Prasanna, S. S. (2018). Virtual reality and noninvasive brain stimulation in stroke: how effective is their combination for upper limb motor improvement?—A meta-analysis. PM&R 10, 1261–1270. doi: 10.1016/j.pmrj.2018.10.001

PubMed Abstract | Crossref Full Text | Google Scholar

Sun, J., Yan, F., Liu, A., Liu, T., and Wang, H. (2021). Electrical stimulation of the motor cortex or paretic muscles improves strength production in stroke patients: a systematic review and meta-analysis. PM&R 13, 171–179. doi: 10.1002/pmrj.12399

PubMed Abstract | Crossref Full Text | Google Scholar

Tang, X., Zhang, N., Shen, Z., Guo, X., Xing, J., Tian, S., et al. (2024). Transcranial direct current stimulation for upper extremity motor dysfunction in poststroke patients: a systematic review and meta-analysis. Clin. Rehabil. 38, 749–769. doi: 10.1177/02692155241235336

PubMed Abstract | Crossref Full Text | Google Scholar

Tedesco Triccas, L., Burridge, J. H., Hughes, A. M., Pickering, R. M., Desikan, M., Rothwell, J. C., et al. (2016). Multiple sessions of transcranial direct current stimulation and upper extremity rehabilitation in stroke: a review and meta-analysis. Clin. Neurophysiol. 127, 946–955. doi: 10.1016/j.clinph.2015.04.067

PubMed Abstract | Crossref Full Text | Google Scholar

The Lancet (2019). ICD-11. Lancet 393:2275. doi: 10.1016/S0140-6736(19)31205-X

PubMed Abstract | Crossref Full Text | Google Scholar

Tien, H. H., Liu, W. Y., Chen, Y. L., Wu, Y. C., and Lien, H. Y. (2020). Transcranial direct current stimulation for improving ambulation after stroke: a systematic review and meta-analysis. Int. J. Rehabil. Res. 43, 299–309. doi: 10.1097/MRR.0000000000000427

PubMed Abstract | Crossref Full Text | Google Scholar

Tung, Y. C., Lai, C. H., Liao, C. D., Huang, S. W., Liou, T. H., and Chen, H. C. (2019). Repetitive transcranial magnetic stimulation of lower limb motor function in patients with stroke: a systematic review and meta-analysis of randomized controlled trials. Clin. Rehabil. 33, 1102–1112. doi: 10.1177/0269215519835889

PubMed Abstract | Crossref Full Text | Google Scholar

Usman, J. S., Wong, T. W.-L., and Ng, S. S. M. (2024). Effects of treadmill training combined with transcranial direct current stimulation on mobility, motor performance, balance function, and other brain-related outcomes in stroke survivors: a systematic review and meta-analysis. Neurol. Sci. 46, 99–111. doi: 10.1007/s10072-024-07768-2

PubMed Abstract | Crossref Full Text | Google Scholar

Van Hoornweder, S., Vanderzande, L., Bloemers, E., Verstraelen, S., Depestele, S., Cuypers, K., et al. (2021). The effects of transcranial direct current stimulation on upper-limb function post-stroke: a meta-analysis of multiple-session studies. Clin. Neurophysiol. 132, 1897–1918. doi: 10.1016/j.clinph.2021.05.015

PubMed Abstract | Crossref Full Text | Google Scholar

van Lieshout, E. C. C., van der Worp, H. B., Visser-Meily, J. M. A., and Dijkhuizen, R. M. (2019). Timing of repetitive transcranial magnetic stimulation onset for upper limb function after stroke: a systematic review and meta-analysis. Front. Neurol. 10:1269. doi: 10.3389/fneur.2019.01269

PubMed Abstract | Crossref Full Text | Google Scholar

Vaz, P. G., Salazar, A. P. D. S., Stein, C., Marchese, R. R., Lukrafka, J. L., Plentz, R. D. M., et al. (2019). Noninvasive brain stimulation combined with other therapies improves gait speed after stroke: a systematic review and meta-analysis. Top. Stroke Rehabil. 26, 201–213. doi: 10.1080/10749357.2019.1565696

PubMed Abstract | Crossref Full Text | Google Scholar

Virani, S. S., Alonso, A., Aparicio, H. J., Benjamin, E. J., Bittencourt, M. S., Callaway, C. W., et al. (2021). Heart disease and stroke statistics-2021 update: a report from the american Heart Association. Circulation 143, e254–e743. doi: 10.1161/CIR.0000000000000950

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, J., Wu, Z., Hong, S., Ye, H., Zhang, Y., Lin, Q., et al. (2024). Cerebellar transcranial magnetic stimulation for improving balance capacity and activity of daily living in stroke patients: a systematic review and meta-analysis. BMC Neurol. 24, 1–16. doi: 10.1186/s12883-024-03720-1

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, X., Huang, G., Wang, D., Sun, L., Leng, H., Zheng, K., et al. (2025). Effects of cerebellar repetitive transcranial magnetic stimulation on stroke rehabilitation: a systematic review and meta-analysis. Brain Res. Bull. 225:111341. doi: 10.1016/j.brainresbull.2025.111341

PubMed Abstract | Crossref Full Text | Google Scholar

Wang, Y., Fong, K. N., Sui, Y., Bai, Z., and Zhang, J. J. (2024). Repetitive peripheral magnetic stimulation alone or in combination with repetitive transcranial magnetic stimulation in poststroke rehabilitation: a systematic review and meta-analysis. J. Neuroeng. Rehabil. 21, 1–16. doi: 10.1186/s12984-024-01486-8

PubMed Abstract | Crossref Full Text | Google Scholar

Wessel, M. J., Draaisma, L. R., Durand-Ruel, M., Maceira-Elvira, P., Moyne, M., Turlan, J. L., et al. (2024). Multi-focal stimulation of the Cortico-cerebellar loop during the acquisition of a novel hand motor skill in chronic stroke survivors. Cerebellum 23, 341–354. doi: 10.1007/s12311-023-01526-4

PubMed Abstract | Crossref Full Text | Google Scholar

Xi, X., Wang, H., Han, L., Ding, M., Li, J., Qiao, C., et al. (2023). Meta-analysis of repetitive transcranial magnetic stimulation combined with task-oriented training on upper limb function in stroke patients with hemiplegia. Medicine 102:e33771. doi: 10.1097/MD.0000000000033771

PubMed Abstract | Crossref Full Text | Google Scholar

Xiang, H., Sun, J., Tang, X., Zeng, K., and Wu, X. (2019). The effect and optimal parameters of repetitive transcranial magnetic stimulation on motor recovery in stroke patients: a systematic review and meta-analysis of randomized controlled trials. Clin. Rehabil. 33, 847–864. doi: 10.1177/0269215519829897

PubMed Abstract | Crossref Full Text | Google Scholar

Xie, G., Wang, T., Deng, L., Zhou, L., Zheng, X., Zhao, C., et al. (2025). Repetitive transcranial magnetic stimulation for motor function in stroke: a systematic review and meta-analysis of randomized controlled studies. Syst. Rev. 14, 1–48. doi: 10.1186/s13643-025-02794-3

PubMed Abstract | Crossref Full Text | Google Scholar

Xie, H., Luo, S., Xiong, D., Zhu, P., Chen, J., Tang, X., et al. (2023). Efficacy and safety of repetitive transcranial magnetic stimulation for poststroke memory disorder: a meta-analysis and systematic review. J. Integr. Neurosci. 22:131. doi: 10.31083/j.jin2205131

PubMed Abstract | Crossref Full Text | Google Scholar

Yu, L., Chen, H., Chen, C., Lin, Y., Huang, Z., Wang, J., et al. (2025). Efficacy of anodal transcranial direct current stimulation for upper extremity function after ischemic stroke: a systematic review of parallel randomized clinical trials. J. Stroke Cerebrovasc. Dis. 34:108112. doi: 10.1016/j.jstrokecerebrovasdis.2024.108112

PubMed Abstract | Crossref Full Text | Google Scholar

Zeng, Y., Ye, Z., Zheng, W., and Wang, J. (2024). Efficacy of cerebellar transcranial magnetic stimulation for post-stroke balance and limb motor function impairments: meta-analyses of random controlled trials and resting-state fMRI studies. Cerebellum 23, 1678–1696. doi: 10.1007/s12311-024-01660-7

PubMed Abstract | Crossref Full Text | Google Scholar

Zhang, J. J., Sui, Y., Sack, A. T., Bai, Z., Kwong, P. W. H., Sanchez Vidana, D. I., et al. (2024). Theta burst stimulation for enhancing upper extremity motor functions after stroke: a systematic review of clinical and mechanistic evidence. Rev. Neurosci. 35, 679–695. doi: 10.1515/revneuro-2024-0030

PubMed Abstract | Crossref Full Text | Google Scholar

Zhang, J. J. Y., Ang, J., Saffari, S. E., Tor, P. C., Lo, Y. L., and Wan, K. R. (2025). Repetitive transcranial magnetic stimulation for motor recovery after stroke: a systematic review and meta-analysis of randomized controlled trials with low risk of bias. Neuromodulation 28, 16–42. doi: 10.1016/j.neurom.2024.07.010

PubMed Abstract | Crossref Full Text | Google Scholar

Zhang, L., Xing, G., Fan, Y., Guo, Z., Chen, H., and Mu, Q. (2017b). Short- and long-term effects of repetitive transcranial magnetic stimulation on upper limb motor function after stroke: a systematic review and meta-analysis. Clin. Rehabil. 31, 1137–1153. doi: 10.1177/0269215517692386

PubMed Abstract | Crossref Full Text | Google Scholar

Zhang, L., Xing, G., Shuai, S., Guo, Z., Chen, H., McClure, M. A., et al. (2017a). Low-frequency repetitive transcranial magnetic stimulation for stroke-induced upper limb motor deficit: a meta-analysis. Neural Plast. 2017:2758097. doi: 10.1155/2017/2758097

PubMed Abstract | Crossref Full Text | Google Scholar

Zhang, N., Wang, H., Wang, H., and Qie, S. (2024). Impact of the combination of virtual reality and noninvasive brain stimulation on the upper limb motor function of stroke patients: a systematic review and meta-analysis. J. Neuroeng. Rehabil. 21, 1–16. doi: 10.1186/s12984-024-01474-y

PubMed Abstract | Crossref Full Text | Google Scholar

Zhou, J., Chen, Y., Gin, T., Bao, D., and Zhou, J. (2023). The effects of repetitive transcranial magnetic stimulation on standing balance and walking in older adults with age-related neurological disorders: a systematic review and meta-analysis. J. Gerontol. A Biol. Sci. Med. Sci. 78, 842–852. doi: 10.1093/gerona/glac158

PubMed Abstract | Crossref Full Text | Google Scholar

Zhu, M., Huang, S., Chen, W., Pan, G., and Zhou, Y. (2024). The effect of transcranial magnetic stimulation on cognitive function in post-stroke patients: a systematic review and meta-analysis. BMC Neurol. 24:234. doi: 10.1186/s12883-024-03726-9

PubMed Abstract | Crossref Full Text | Google Scholar

Zieliński, G. (2025). Effect size guidelines for individual and group differences in physiotherapy. Arch. Phys. Med. Rehabil. 19, 717–718. doi: 10.1016/j.apmr.2025.05.013

PubMed Abstract | Crossref Full Text | Google Scholar